Vasoprotective Endothelial Effects of Chronic Cannabidiol Treatment and Its Influence on the Endocannabinoid System in Rats with Primary and Secondary Hypertension

Our study aimed to examine the endothelium (vascular)-protecting effects of chronic cannabidiol (CBD) administration (10 mg/kg once daily for 2 weeks) in aortas and small mesenteric (G3) arteries isolated from deoxycorticosterone-induced hypertensive (DOCA-salt) rats and spontaneously hypertensive r...

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Autores principales:	Marta Baranowska-Kuczko, Hanna Kozłowska, Monika Kloza, Magdalena Kusaczuk, Ewa Harasim-Symbor, Michał Biernacki, Irena Kasacka, Barbara Malinowska
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cannabidiol vascular (endothelial) dysfunction SHR DOCA-salt endocannabinoids eNOS Medicine R Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/774e252271d24492b51f6bf46136cf34
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spelling	oai:doaj.org-article:774e252271d24492b51f6bf46136cf342021-11-25T18:39:30ZVasoprotective Endothelial Effects of Chronic Cannabidiol Treatment and Its Influence on the Endocannabinoid System in Rats with Primary and Secondary Hypertension10.3390/ph141111201424-8247https://doaj.org/article/774e252271d24492b51f6bf46136cf342021-10-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1120https://doaj.org/toc/1424-8247Our study aimed to examine the endothelium (vascular)-protecting effects of chronic cannabidiol (CBD) administration (10 mg/kg once daily for 2 weeks) in aortas and small mesenteric (G3) arteries isolated from deoxycorticosterone-induced hypertensive (DOCA-salt) rats and spontaneously hypertensive rats (SHR). CBD reduced hypertrophy and improved the endothelium-dependent vasodilation in response to acetylcholine in the aortas and G3 of DOCA-salt rats and SHR. The enhancement of vasorelaxation was prevented by the inhibition of nitric oxide (NO) with L-NAME and/or the inhibition of cyclooxygenase (COX) with indomethacin in the aortas and G3 of DOCA-salt and SHR, respectively. The mechanism of the CBD-mediated improvement of endothelial function in hypertensive vessels depends on the vessel diameter and may be associated with its NO-, the intermediate-conductance calcium-activated potassium channel- or NO-, COX-, the intermediate and the small-conductance calcium-activated potassium channels-dependent effect in aortas and G3, respectively. CBD increased the vascular expression of the cannabinoid CB<sub>1</sub> and CB<sub>2</sub> receptors and aortic levels of endocannabinoids with vasorelaxant properties e.g., anandamide, 2-arachidonoylglycerol and palmitoyl ethanolamide in aortas of DOCA-salt and/or SHR. In conclusion, CBD treatment has vasoprotective effects in hypertensive rats, in a vessel-size- and hypertension-model-independent manner, at least partly via inducing local vascular changes in the endocannabinoid system.Marta Baranowska-KuczkoHanna KozłowskaMonika KlozaMagdalena KusaczukEwa Harasim-SymborMichał BiernackiIrena KasackaBarbara MalinowskaMDPI AGarticlecannabidiolvascular (endothelial) dysfunctionSHRDOCA-saltendocannabinoidseNOSMedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1120, p 1120 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cannabidiol vascular (endothelial) dysfunction SHR DOCA-salt endocannabinoids eNOS Medicine R Pharmacy and materia medica RS1-441
spellingShingle	cannabidiol vascular (endothelial) dysfunction SHR DOCA-salt endocannabinoids eNOS Medicine R Pharmacy and materia medica RS1-441 Marta Baranowska-Kuczko Hanna Kozłowska Monika Kloza Magdalena Kusaczuk Ewa Harasim-Symbor Michał Biernacki Irena Kasacka Barbara Malinowska Vasoprotective Endothelial Effects of Chronic Cannabidiol Treatment and Its Influence on the Endocannabinoid System in Rats with Primary and Secondary Hypertension
description	Our study aimed to examine the endothelium (vascular)-protecting effects of chronic cannabidiol (CBD) administration (10 mg/kg once daily for 2 weeks) in aortas and small mesenteric (G3) arteries isolated from deoxycorticosterone-induced hypertensive (DOCA-salt) rats and spontaneously hypertensive rats (SHR). CBD reduced hypertrophy and improved the endothelium-dependent vasodilation in response to acetylcholine in the aortas and G3 of DOCA-salt rats and SHR. The enhancement of vasorelaxation was prevented by the inhibition of nitric oxide (NO) with L-NAME and/or the inhibition of cyclooxygenase (COX) with indomethacin in the aortas and G3 of DOCA-salt and SHR, respectively. The mechanism of the CBD-mediated improvement of endothelial function in hypertensive vessels depends on the vessel diameter and may be associated with its NO-, the intermediate-conductance calcium-activated potassium channel- or NO-, COX-, the intermediate and the small-conductance calcium-activated potassium channels-dependent effect in aortas and G3, respectively. CBD increased the vascular expression of the cannabinoid CB<sub>1</sub> and CB<sub>2</sub> receptors and aortic levels of endocannabinoids with vasorelaxant properties e.g., anandamide, 2-arachidonoylglycerol and palmitoyl ethanolamide in aortas of DOCA-salt and/or SHR. In conclusion, CBD treatment has vasoprotective effects in hypertensive rats, in a vessel-size- and hypertension-model-independent manner, at least partly via inducing local vascular changes in the endocannabinoid system.
format	article
author	Marta Baranowska-Kuczko Hanna Kozłowska Monika Kloza Magdalena Kusaczuk Ewa Harasim-Symbor Michał Biernacki Irena Kasacka Barbara Malinowska
author_facet	Marta Baranowska-Kuczko Hanna Kozłowska Monika Kloza Magdalena Kusaczuk Ewa Harasim-Symbor Michał Biernacki Irena Kasacka Barbara Malinowska
author_sort	Marta Baranowska-Kuczko
title	Vasoprotective Endothelial Effects of Chronic Cannabidiol Treatment and Its Influence on the Endocannabinoid System in Rats with Primary and Secondary Hypertension
title_short	Vasoprotective Endothelial Effects of Chronic Cannabidiol Treatment and Its Influence on the Endocannabinoid System in Rats with Primary and Secondary Hypertension
title_full	Vasoprotective Endothelial Effects of Chronic Cannabidiol Treatment and Its Influence on the Endocannabinoid System in Rats with Primary and Secondary Hypertension
title_fullStr	Vasoprotective Endothelial Effects of Chronic Cannabidiol Treatment and Its Influence on the Endocannabinoid System in Rats with Primary and Secondary Hypertension
title_full_unstemmed	Vasoprotective Endothelial Effects of Chronic Cannabidiol Treatment and Its Influence on the Endocannabinoid System in Rats with Primary and Secondary Hypertension
title_sort	vasoprotective endothelial effects of chronic cannabidiol treatment and its influence on the endocannabinoid system in rats with primary and secondary hypertension
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/774e252271d24492b51f6bf46136cf34
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Vasoprotective Endothelial Effects of Chronic Cannabidiol Treatment and Its Influence on the Endocannabinoid System in Rats with Primary and Secondary Hypertension

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