Immunoinformatics-Based Designing of a Multi-Epitope Chimeric Vaccine From Multi-Domain Outer Surface Antigens of Leptospira

Accurate information on antigenic epitopes within a multi-domain antigen would provide insights into vaccine design and immunotherapy. The multi-domain outer surface Leptospira immunoglobulin-like (Lig) proteins LigA and LigB, consisting of 12–13 homologous bacterial Ig (Big)-like domains, are poten...

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Autores principales:	Pankaj Kumar, Surabhi Lata, Umate Nachiket Shankar, Mohd. Akif
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Leptospira interrogans antigenic epitope outer surface antigen vaccine Leptospira immunoglobulin-like protein subunit vaccine Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/775527df88bd4ecda4b4b9490b902535
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spelling	oai:doaj.org-article:775527df88bd4ecda4b4b9490b9025352021-12-01T19:39:14ZImmunoinformatics-Based Designing of a Multi-Epitope Chimeric Vaccine From Multi-Domain Outer Surface Antigens of Leptospira1664-322410.3389/fimmu.2021.735373https://doaj.org/article/775527df88bd4ecda4b4b9490b9025352021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.735373/fullhttps://doaj.org/toc/1664-3224Accurate information on antigenic epitopes within a multi-domain antigen would provide insights into vaccine design and immunotherapy. The multi-domain outer surface Leptospira immunoglobulin-like (Lig) proteins LigA and LigB, consisting of 12–13 homologous bacterial Ig (Big)-like domains, are potential antigens of Leptospira interrogans. Currently, no effective vaccine is available against pathogenic Leptospira. Both the humoral immunity and cell-mediated immunity of the host play critical roles in defending against Leptospira infection. Here, we used immunoinformatics approaches to evaluate antigenic B-cell lymphocyte (BCL) and cytotoxic T-lymphocyte (CTL) epitopes from Lig proteins. Based on certain crucial parameters, potential epitopes that can stimulate both types of adaptive immune responses were selected to design a chimeric vaccine construct. Additionally, an adjuvant, the mycobacterial heparin-binding hemagglutinin adhesin (HBHA), was incorporated into the final multi-epitope vaccine construct with a suitable linker. The final construct was further scored for its antigenicity, allergenicity, and physicochemical parameters. A three-dimensional (3D) modeled construct of the vaccine was implied to interact with Toll-like receptor 4 (TLR4) using molecular docking. The stability of the vaccine construct with TLR4 was predicted with molecular dynamics simulation. Our results demonstrate the application of immunoinformatics and structure biology strategies to develop an epitope-specific chimeric vaccine from multi-domain proteins. The current findings will be useful for future experimental validation to ratify the immunogenicity of the chimera.Pankaj KumarSurabhi LataUmate Nachiket ShankarMohd. AkifFrontiers Media S.A.articleLeptospira interrogansantigenic epitopeouter surface antigenvaccineLeptospira immunoglobulin-like proteinsubunit vaccineImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Leptospira interrogans antigenic epitope outer surface antigen vaccine Leptospira immunoglobulin-like protein subunit vaccine Immunologic diseases. Allergy RC581-607
spellingShingle	Leptospira interrogans antigenic epitope outer surface antigen vaccine Leptospira immunoglobulin-like protein subunit vaccine Immunologic diseases. Allergy RC581-607 Pankaj Kumar Surabhi Lata Umate Nachiket Shankar Mohd. Akif Immunoinformatics-Based Designing of a Multi-Epitope Chimeric Vaccine From Multi-Domain Outer Surface Antigens of Leptospira
description	Accurate information on antigenic epitopes within a multi-domain antigen would provide insights into vaccine design and immunotherapy. The multi-domain outer surface Leptospira immunoglobulin-like (Lig) proteins LigA and LigB, consisting of 12–13 homologous bacterial Ig (Big)-like domains, are potential antigens of Leptospira interrogans. Currently, no effective vaccine is available against pathogenic Leptospira. Both the humoral immunity and cell-mediated immunity of the host play critical roles in defending against Leptospira infection. Here, we used immunoinformatics approaches to evaluate antigenic B-cell lymphocyte (BCL) and cytotoxic T-lymphocyte (CTL) epitopes from Lig proteins. Based on certain crucial parameters, potential epitopes that can stimulate both types of adaptive immune responses were selected to design a chimeric vaccine construct. Additionally, an adjuvant, the mycobacterial heparin-binding hemagglutinin adhesin (HBHA), was incorporated into the final multi-epitope vaccine construct with a suitable linker. The final construct was further scored for its antigenicity, allergenicity, and physicochemical parameters. A three-dimensional (3D) modeled construct of the vaccine was implied to interact with Toll-like receptor 4 (TLR4) using molecular docking. The stability of the vaccine construct with TLR4 was predicted with molecular dynamics simulation. Our results demonstrate the application of immunoinformatics and structure biology strategies to develop an epitope-specific chimeric vaccine from multi-domain proteins. The current findings will be useful for future experimental validation to ratify the immunogenicity of the chimera.
format	article
author	Pankaj Kumar Surabhi Lata Umate Nachiket Shankar Mohd. Akif
author_facet	Pankaj Kumar Surabhi Lata Umate Nachiket Shankar Mohd. Akif
author_sort	Pankaj Kumar
title	Immunoinformatics-Based Designing of a Multi-Epitope Chimeric Vaccine From Multi-Domain Outer Surface Antigens of Leptospira
title_short	Immunoinformatics-Based Designing of a Multi-Epitope Chimeric Vaccine From Multi-Domain Outer Surface Antigens of Leptospira
title_full	Immunoinformatics-Based Designing of a Multi-Epitope Chimeric Vaccine From Multi-Domain Outer Surface Antigens of Leptospira
title_fullStr	Immunoinformatics-Based Designing of a Multi-Epitope Chimeric Vaccine From Multi-Domain Outer Surface Antigens of Leptospira
title_full_unstemmed	Immunoinformatics-Based Designing of a Multi-Epitope Chimeric Vaccine From Multi-Domain Outer Surface Antigens of Leptospira
title_sort	immunoinformatics-based designing of a multi-epitope chimeric vaccine from multi-domain outer surface antigens of leptospira
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/775527df88bd4ecda4b4b9490b902535
work_keys_str_mv	AT pankajkumar immunoinformaticsbaseddesigningofamultiepitopechimericvaccinefrommultidomainoutersurfaceantigensofleptospira AT surabhilata immunoinformaticsbaseddesigningofamultiepitopechimericvaccinefrommultidomainoutersurfaceantigensofleptospira AT umatenachiketshankar immunoinformaticsbaseddesigningofamultiepitopechimericvaccinefrommultidomainoutersurfaceantigensofleptospira AT mohdakif immunoinformaticsbaseddesigningofamultiepitopechimericvaccinefrommultidomainoutersurfaceantigensofleptospira
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Immunoinformatics-Based Designing of a Multi-Epitope Chimeric Vaccine From Multi-Domain Outer Surface Antigens of Leptospira

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