Nanostructured lipid carrier-loaded hyaluronic acid microneedles for controlled dermal delivery of a lipophilic molecule

Sang Gon Lee,1,* Jae Han Jeong,1,* Kyung Min Lee,1 Kyu Ho Jeong,1 Huisuk Yang,2 Miroo Kim,2 Hyungil Jung,2 Sangkil Lee,3 Young Wook Choi11College of Pharmacy, Chung-Ang University, Seoul, South Korea; 2Department of Biotechnology, Yonsei University, Seoul, South Korea; 3College of Pharmacy, Keimyun...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lee SG, Jeong JH, Lee KM, Jeong KH, Yang H, Kim M, Jung H, Lee S, Choi YW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/775985cf68094920b554a583c9109e05
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:775985cf68094920b554a583c9109e05
record_format	dspace
spelling	oai:doaj.org-article:775985cf68094920b554a583c9109e052021-12-02T00:36:57ZNanostructured lipid carrier-loaded hyaluronic acid microneedles for controlled dermal delivery of a lipophilic molecule1178-2013https://doaj.org/article/775985cf68094920b554a583c9109e052013-12-01T00:00:00Zhttp://www.dovepress.com/nanostructured-lipid-carrier-loaded-hyaluronic-acid-microneedles-for-c-a15408https://doaj.org/toc/1178-2013 Sang Gon Lee,1,* Jae Han Jeong,1,* Kyung Min Lee,1 Kyu Ho Jeong,1 Huisuk Yang,2 Miroo Kim,2 Hyungil Jung,2 Sangkil Lee,3 Young Wook Choi11College of Pharmacy, Chung-Ang University, Seoul, South Korea; 2Department of Biotechnology, Yonsei University, Seoul, South Korea; 3College of Pharmacy, Keimyung University, Daegu, South Korea *These authors contributed equally to this workAbstract: Nanostructured lipid carriers (NLCs) were employed to formulate a lipophilic drug into hydrophilic polymeric microneedles (MNs). Hyaluronic acid (HA) was selected as a hydrophilic and bioerodible polymer to fabricate MNs, and nile red (NR) was used as a model lipophilic molecule. NR-loaded NLCs were consolidated into the HA-based MNs to prepare NLC-loaded MNs (NLC-MNs). A dispersion of NLCs was prepared by high-pressure homogenization after dissolving NR in Labrafil and mixing with melted Compritol, resulting in 268 nm NLCs with a polydispersity index of 0.273. The NLC dispersion showed a controlled release of NR over 24 hours, following Hixson–Crowell's cube root law. After mixing the NLC dispersion with the HA solution, the drawing lithography method was used to fabricate NLC-MNs. The length, base diameter, and tip diameter of the NLC-MNs were approximately 350, 380, and 30 µm, respectively. Fluorescence microscopic imaging of the NLC-MNs helped confirm that the NR-loaded NLCs were distributed evenly throughout the MNs. In a skin permeation study performed using a Franz diffusion cell with minipig dorsal skin, approximately 70% of NR was localized in the skin after 24-hour application of NLC-MNs. Confocal laser scanning microscopy (z-series) of the skin at different depths showed strong fluorescence intensity in the epidermal layer, which appeared to spread out radially with the passage of time. This study indicated that incorporation of drug-loaded NLCs into MNs could represent a promising strategy for controlled dermal delivery of lipophilic drugs.Keywords: polymeric microneedles, nanostructured lipid carriers, dermal delivery, controlled release, hyaluronic acidLee SGJeong JHLee KMJeong KHYang HKim MJung HLee SChoi YWDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 289-299 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Lee SG Jeong JH Lee KM Jeong KH Yang H Kim M Jung H Lee S Choi YW Nanostructured lipid carrier-loaded hyaluronic acid microneedles for controlled dermal delivery of a lipophilic molecule
description	Sang Gon Lee,1,* Jae Han Jeong,1,* Kyung Min Lee,1 Kyu Ho Jeong,1 Huisuk Yang,2 Miroo Kim,2 Hyungil Jung,2 Sangkil Lee,3 Young Wook Choi11College of Pharmacy, Chung-Ang University, Seoul, South Korea; 2Department of Biotechnology, Yonsei University, Seoul, South Korea; 3College of Pharmacy, Keimyung University, Daegu, South Korea *These authors contributed equally to this workAbstract: Nanostructured lipid carriers (NLCs) were employed to formulate a lipophilic drug into hydrophilic polymeric microneedles (MNs). Hyaluronic acid (HA) was selected as a hydrophilic and bioerodible polymer to fabricate MNs, and nile red (NR) was used as a model lipophilic molecule. NR-loaded NLCs were consolidated into the HA-based MNs to prepare NLC-loaded MNs (NLC-MNs). A dispersion of NLCs was prepared by high-pressure homogenization after dissolving NR in Labrafil and mixing with melted Compritol, resulting in 268 nm NLCs with a polydispersity index of 0.273. The NLC dispersion showed a controlled release of NR over 24 hours, following Hixson–Crowell's cube root law. After mixing the NLC dispersion with the HA solution, the drawing lithography method was used to fabricate NLC-MNs. The length, base diameter, and tip diameter of the NLC-MNs were approximately 350, 380, and 30 µm, respectively. Fluorescence microscopic imaging of the NLC-MNs helped confirm that the NR-loaded NLCs were distributed evenly throughout the MNs. In a skin permeation study performed using a Franz diffusion cell with minipig dorsal skin, approximately 70% of NR was localized in the skin after 24-hour application of NLC-MNs. Confocal laser scanning microscopy (z-series) of the skin at different depths showed strong fluorescence intensity in the epidermal layer, which appeared to spread out radially with the passage of time. This study indicated that incorporation of drug-loaded NLCs into MNs could represent a promising strategy for controlled dermal delivery of lipophilic drugs.Keywords: polymeric microneedles, nanostructured lipid carriers, dermal delivery, controlled release, hyaluronic acid
format	article
author	Lee SG Jeong JH Lee KM Jeong KH Yang H Kim M Jung H Lee S Choi YW
author_facet	Lee SG Jeong JH Lee KM Jeong KH Yang H Kim M Jung H Lee S Choi YW
author_sort	Lee SG
title	Nanostructured lipid carrier-loaded hyaluronic acid microneedles for controlled dermal delivery of a lipophilic molecule
title_short	Nanostructured lipid carrier-loaded hyaluronic acid microneedles for controlled dermal delivery of a lipophilic molecule
title_full	Nanostructured lipid carrier-loaded hyaluronic acid microneedles for controlled dermal delivery of a lipophilic molecule
title_fullStr	Nanostructured lipid carrier-loaded hyaluronic acid microneedles for controlled dermal delivery of a lipophilic molecule
title_full_unstemmed	Nanostructured lipid carrier-loaded hyaluronic acid microneedles for controlled dermal delivery of a lipophilic molecule
title_sort	nanostructured lipid carrier-loaded hyaluronic acid microneedles for controlled dermal delivery of a lipophilic molecule
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/775985cf68094920b554a583c9109e05
work_keys_str_mv	AT leesg nanostructuredlipidcarrierloadedhyaluronicacidmicroneedlesforcontrolleddermaldeliveryofalipophilicmolecule AT jeongjh nanostructuredlipidcarrierloadedhyaluronicacidmicroneedlesforcontrolleddermaldeliveryofalipophilicmolecule AT leekm nanostructuredlipidcarrierloadedhyaluronicacidmicroneedlesforcontrolleddermaldeliveryofalipophilicmolecule AT jeongkh nanostructuredlipidcarrierloadedhyaluronicacidmicroneedlesforcontrolleddermaldeliveryofalipophilicmolecule AT yangh nanostructuredlipidcarrierloadedhyaluronicacidmicroneedlesforcontrolleddermaldeliveryofalipophilicmolecule AT kimm nanostructuredlipidcarrierloadedhyaluronicacidmicroneedlesforcontrolleddermaldeliveryofalipophilicmolecule AT jungh nanostructuredlipidcarrierloadedhyaluronicacidmicroneedlesforcontrolleddermaldeliveryofalipophilicmolecule AT lees nanostructuredlipidcarrierloadedhyaluronicacidmicroneedlesforcontrolleddermaldeliveryofalipophilicmolecule AT choiyw nanostructuredlipidcarrierloadedhyaluronicacidmicroneedlesforcontrolleddermaldeliveryofalipophilicmolecule
_version_	1718403626699849728

Nanostructured lipid carrier-loaded hyaluronic acid microneedles for controlled dermal delivery of a lipophilic molecule

Ejemplares similares