MONOCYTES SUBPOPULATIONS AND CHEMILUMINESCENT ACTIVITY IN PATIENTS WITH RENAL CELL CARCINOMA

The aim of present study was to investigate the relationship between phenotypic features of monocytes and intensity of “respiratory burst” in the patients with renal cell carcinoma (RCC). A total of 73 patients with RCC (Т3N0М0, clear cell type) participated in the study. Phenotyping of blood monocy...

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Autores principales:	A. A. Savchenko, A. G. Borisov, A. A. Modestov, A. V. Moshev, I. V. Kudryavtsev, O. G. Tonacheva, V. N. Koshcheev
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2015
Materias:	renal cancer cells monocytes subpopulations functional activity activation markers respiratory burst Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/775a3c5b964d4bbeb1b02a1a4ac42526
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spelling	oai:doaj.org-article:775a3c5b964d4bbeb1b02a1a4ac425262021-11-18T08:03:44ZMONOCYTES SUBPOPULATIONS AND CHEMILUMINESCENT ACTIVITY IN PATIENTS WITH RENAL CELL CARCINOMA1563-06252313-741X10.15789/1563-0625-2015-2-141-150https://doaj.org/article/775a3c5b964d4bbeb1b02a1a4ac425262015-04-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/832https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe aim of present study was to investigate the relationship between phenotypic features of monocytes and intensity of “respiratory burst” in the patients with renal cell carcinoma (RCC). A total of 73 patients with RCC (Т3N0М0, clear cell type) participated in the study. Phenotyping of blood monocytes was performed by flow cytometry. The level of “respiratory burst” in monocytes was determined using spontaneous and zymosan-induced luminol- and lucigenin-dependent chemiluminescence. Suffficient changes in phenotypic structure and intensity of “respiratory burst” were identified in peripheral blood monocytes of the patients. Alterations of monocytic subpopulations in RCC were characterized by increased numbers of cells with the CD14lowCD16+ (“non-classical”) phenotype. The imbalance in expression of activation markers was found among monocyte populations from cancer patients; we have revealed a reduced number of monocytes expressing HLA-DR-antigen, and increased number of CD64-positive cells. Meanwhile, intensity of “respiratory burst” in the total monocyte population proved to be reduced in RCC patients. In this case, the characteristic features of the “respiratory burst” intensity distribution among monocytes were as follows: in RCC, a reduced “respiratory burst” activity was found in monocytes with CD14+CD16- phenotype, being, however, increased in the monocytes with CD14+CD16+ and CD14lowCD16+ phenotypes. Such redistribution may be due to increasing role of the given monocyte subsets in immunopathogenesis of renal cell carcinoma.A. A. SavchenkoA. G. BorisovA. A. ModestovA. V. MoshevI. V. KudryavtsevO. G. TonachevaV. N. KoshcheevSPb RAACIarticlerenal cancer cellsmonocytessubpopulationsfunctional activityactivation markersrespiratory burstImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 17, Iss 2, Pp 141-150 (2015)
institution	DOAJ
collection	DOAJ
language	RU
topic	renal cancer cells monocytes subpopulations functional activity activation markers respiratory burst Immunologic diseases. Allergy RC581-607
spellingShingle	renal cancer cells monocytes subpopulations functional activity activation markers respiratory burst Immunologic diseases. Allergy RC581-607 A. A. Savchenko A. G. Borisov A. A. Modestov A. V. Moshev I. V. Kudryavtsev O. G. Tonacheva V. N. Koshcheev MONOCYTES SUBPOPULATIONS AND CHEMILUMINESCENT ACTIVITY IN PATIENTS WITH RENAL CELL CARCINOMA
description	The aim of present study was to investigate the relationship between phenotypic features of monocytes and intensity of “respiratory burst” in the patients with renal cell carcinoma (RCC). A total of 73 patients with RCC (Т3N0М0, clear cell type) participated in the study. Phenotyping of blood monocytes was performed by flow cytometry. The level of “respiratory burst” in monocytes was determined using spontaneous and zymosan-induced luminol- and lucigenin-dependent chemiluminescence. Suffficient changes in phenotypic structure and intensity of “respiratory burst” were identified in peripheral blood monocytes of the patients. Alterations of monocytic subpopulations in RCC were characterized by increased numbers of cells with the CD14lowCD16+ (“non-classical”) phenotype. The imbalance in expression of activation markers was found among monocyte populations from cancer patients; we have revealed a reduced number of monocytes expressing HLA-DR-antigen, and increased number of CD64-positive cells. Meanwhile, intensity of “respiratory burst” in the total monocyte population proved to be reduced in RCC patients. In this case, the characteristic features of the “respiratory burst” intensity distribution among monocytes were as follows: in RCC, a reduced “respiratory burst” activity was found in monocytes with CD14+CD16- phenotype, being, however, increased in the monocytes with CD14+CD16+ and CD14lowCD16+ phenotypes. Such redistribution may be due to increasing role of the given monocyte subsets in immunopathogenesis of renal cell carcinoma.
format	article
author	A. A. Savchenko A. G. Borisov A. A. Modestov A. V. Moshev I. V. Kudryavtsev O. G. Tonacheva V. N. Koshcheev
author_facet	A. A. Savchenko A. G. Borisov A. A. Modestov A. V. Moshev I. V. Kudryavtsev O. G. Tonacheva V. N. Koshcheev
author_sort	A. A. Savchenko
title	MONOCYTES SUBPOPULATIONS AND CHEMILUMINESCENT ACTIVITY IN PATIENTS WITH RENAL CELL CARCINOMA
title_short	MONOCYTES SUBPOPULATIONS AND CHEMILUMINESCENT ACTIVITY IN PATIENTS WITH RENAL CELL CARCINOMA
title_full	MONOCYTES SUBPOPULATIONS AND CHEMILUMINESCENT ACTIVITY IN PATIENTS WITH RENAL CELL CARCINOMA
title_fullStr	MONOCYTES SUBPOPULATIONS AND CHEMILUMINESCENT ACTIVITY IN PATIENTS WITH RENAL CELL CARCINOMA
title_full_unstemmed	MONOCYTES SUBPOPULATIONS AND CHEMILUMINESCENT ACTIVITY IN PATIENTS WITH RENAL CELL CARCINOMA
title_sort	monocytes subpopulations and chemiluminescent activity in patients with renal cell carcinoma
publisher	SPb RAACI
publishDate	2015
url	https://doaj.org/article/775a3c5b964d4bbeb1b02a1a4ac42526
work_keys_str_mv	AT aasavchenko monocytessubpopulationsandchemiluminescentactivityinpatientswithrenalcellcarcinoma AT agborisov monocytessubpopulationsandchemiluminescentactivityinpatientswithrenalcellcarcinoma AT aamodestov monocytessubpopulationsandchemiluminescentactivityinpatientswithrenalcellcarcinoma AT avmoshev monocytessubpopulationsandchemiluminescentactivityinpatientswithrenalcellcarcinoma AT ivkudryavtsev monocytessubpopulationsandchemiluminescentactivityinpatientswithrenalcellcarcinoma AT ogtonacheva monocytessubpopulationsandchemiluminescentactivityinpatientswithrenalcellcarcinoma AT vnkoshcheev monocytessubpopulationsandchemiluminescentactivityinpatientswithrenalcellcarcinoma
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MONOCYTES SUBPOPULATIONS AND CHEMILUMINESCENT ACTIVITY IN PATIENTS WITH RENAL CELL CARCINOMA

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