An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria

Abstract Flavin-binding fluorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efficacy of eleven LOV-based FPs. In particular, we deter...

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Autores principales:	Stephan Endres, Marcus Wingen, Joaquim Torra, Rubén Ruiz-González, Tino Polen, Gabriela Bosio, Nora Lisa Bitzenhofer, Fabienne Hilgers, Thomas Gensch, Santi Nonell, Karl-Erich Jaeger, Thomas Drepper
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Optogenetic miniSOG BioLector Blue Light Illumination High Phototoxicity Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7763452b005e45089d306d2fa553c16f
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spelling	oai:doaj.org-article:7763452b005e45089d306d2fa553c16f2021-12-02T15:07:49ZAn optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria10.1038/s41598-018-33291-42045-2322https://doaj.org/article/7763452b005e45089d306d2fa553c16f2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33291-4https://doaj.org/toc/2045-2322Abstract Flavin-binding fluorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efficacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate 1O2, all of the other tested flavoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable differences in ROS selectivity and yield. Accordingly, most LOV-FPs are potent photosensitizers, which can be used for light-controlled killing of bacteria. Furthermore, the two variants Pp2FbFP and DsFbFP M49I, exhibiting preferential photosensitization of singlet oxygen or singlet oxygen and superoxide, respectively, were shown to be new tools for studying specific ROS-induced cell signaling processes. The tested LOV-FPs thus further expand the toolbox of optogenetic sensitizers usable for a broad spectrum of microbiological and biomedical applications.Stephan EndresMarcus WingenJoaquim TorraRubén Ruiz-GonzálezTino PolenGabriela BosioNora Lisa BitzenhoferFabienne HilgersThomas GenschSanti NonellKarl-Erich JaegerThomas DrepperNature PortfolioarticleOptogeneticminiSOGBioLectorBlue Light IlluminationHigh PhototoxicityMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Optogenetic miniSOG BioLector Blue Light Illumination High Phototoxicity Medicine R Science Q
spellingShingle	Optogenetic miniSOG BioLector Blue Light Illumination High Phototoxicity Medicine R Science Q Stephan Endres Marcus Wingen Joaquim Torra Rubén Ruiz-González Tino Polen Gabriela Bosio Nora Lisa Bitzenhofer Fabienne Hilgers Thomas Gensch Santi Nonell Karl-Erich Jaeger Thomas Drepper An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
description	Abstract Flavin-binding fluorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efficacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate 1O2, all of the other tested flavoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable differences in ROS selectivity and yield. Accordingly, most LOV-FPs are potent photosensitizers, which can be used for light-controlled killing of bacteria. Furthermore, the two variants Pp2FbFP and DsFbFP M49I, exhibiting preferential photosensitization of singlet oxygen or singlet oxygen and superoxide, respectively, were shown to be new tools for studying specific ROS-induced cell signaling processes. The tested LOV-FPs thus further expand the toolbox of optogenetic sensitizers usable for a broad spectrum of microbiological and biomedical applications.
format	article
author	Stephan Endres Marcus Wingen Joaquim Torra Rubén Ruiz-González Tino Polen Gabriela Bosio Nora Lisa Bitzenhofer Fabienne Hilgers Thomas Gensch Santi Nonell Karl-Erich Jaeger Thomas Drepper
author_facet	Stephan Endres Marcus Wingen Joaquim Torra Rubén Ruiz-González Tino Polen Gabriela Bosio Nora Lisa Bitzenhofer Fabienne Hilgers Thomas Gensch Santi Nonell Karl-Erich Jaeger Thomas Drepper
author_sort	Stephan Endres
title	An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_short	An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_full	An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_fullStr	An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_full_unstemmed	An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
title_sort	optogenetic toolbox of lov-based photosensitizers for light-driven killing of bacteria
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/7763452b005e45089d306d2fa553c16f
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An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria

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