Mesenchymal stem cell-derived exosomes protect trabecular meshwork from oxidative stress

Abstract This study aims to investigate the beneficial effects of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) on trabecular meshwork cells under oxidative stress and predict candidate genes associated with this process. Trabecular meshwork cells were pretreated with BMSC-derived...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ying-chao Li, Juan Zheng, Xi-zi Wang, Xin Wang, Wen-jing Liu, Jian-lu Gao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7766c152f12c4634bd6a365d5e939c08
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7766c152f12c4634bd6a365d5e939c08
record_format	dspace
spelling	oai:doaj.org-article:7766c152f12c4634bd6a365d5e939c082021-12-02T16:26:29ZMesenchymal stem cell-derived exosomes protect trabecular meshwork from oxidative stress10.1038/s41598-021-94365-42045-2322https://doaj.org/article/7766c152f12c4634bd6a365d5e939c082021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94365-4https://doaj.org/toc/2045-2322Abstract This study aims to investigate the beneficial effects of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) on trabecular meshwork cells under oxidative stress and predict candidate genes associated with this process. Trabecular meshwork cells were pretreated with BMSC-derived exosomes for 24 h, and exposed to 0.1 mM H2O2 for 6 h. Survival rate of trabecular meshwork cells was measured with CCK-8 assay. Production of intracellular reactive oxygen species (iROS) was measured using a flow cytometer. RT-PCR and ELISA were used to detect mRNA and protein levels of inflammatory cytokines and matrix metalloproteinases (MMPs). Sequencing of RNA and miRNA for trabecular meshwork cells from Exo and control groups was performed on BGISEQ500 platform. Phenotypically, pretreatment of BMSC-derived exosomes improves survival rate of trabecular meshwork cells exposed to H2O2, reduces production of iROS, and inhibits expression of inflammatory cytokines, whereas increases expression of MMPs. There were 23 miRNAs, 307 lncRNAs, and 367 mRNAs differentially expressed between Exo and control groups. Exosomes derived from BMSCs may protect trabecular meshwork cells from oxidative stress. Candidate genes responsible for beneficial effects, such as DIO2 and HMOX1, were predicted.Ying-chao LiJuan ZhengXi-zi WangXin WangWen-jing LiuJian-lu GaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ying-chao Li Juan Zheng Xi-zi Wang Xin Wang Wen-jing Liu Jian-lu Gao Mesenchymal stem cell-derived exosomes protect trabecular meshwork from oxidative stress
description	Abstract This study aims to investigate the beneficial effects of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) on trabecular meshwork cells under oxidative stress and predict candidate genes associated with this process. Trabecular meshwork cells were pretreated with BMSC-derived exosomes for 24 h, and exposed to 0.1 mM H2O2 for 6 h. Survival rate of trabecular meshwork cells was measured with CCK-8 assay. Production of intracellular reactive oxygen species (iROS) was measured using a flow cytometer. RT-PCR and ELISA were used to detect mRNA and protein levels of inflammatory cytokines and matrix metalloproteinases (MMPs). Sequencing of RNA and miRNA for trabecular meshwork cells from Exo and control groups was performed on BGISEQ500 platform. Phenotypically, pretreatment of BMSC-derived exosomes improves survival rate of trabecular meshwork cells exposed to H2O2, reduces production of iROS, and inhibits expression of inflammatory cytokines, whereas increases expression of MMPs. There were 23 miRNAs, 307 lncRNAs, and 367 mRNAs differentially expressed between Exo and control groups. Exosomes derived from BMSCs may protect trabecular meshwork cells from oxidative stress. Candidate genes responsible for beneficial effects, such as DIO2 and HMOX1, were predicted.
format	article
author	Ying-chao Li Juan Zheng Xi-zi Wang Xin Wang Wen-jing Liu Jian-lu Gao
author_facet	Ying-chao Li Juan Zheng Xi-zi Wang Xin Wang Wen-jing Liu Jian-lu Gao
author_sort	Ying-chao Li
title	Mesenchymal stem cell-derived exosomes protect trabecular meshwork from oxidative stress
title_short	Mesenchymal stem cell-derived exosomes protect trabecular meshwork from oxidative stress
title_full	Mesenchymal stem cell-derived exosomes protect trabecular meshwork from oxidative stress
title_fullStr	Mesenchymal stem cell-derived exosomes protect trabecular meshwork from oxidative stress
title_full_unstemmed	Mesenchymal stem cell-derived exosomes protect trabecular meshwork from oxidative stress
title_sort	mesenchymal stem cell-derived exosomes protect trabecular meshwork from oxidative stress
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7766c152f12c4634bd6a365d5e939c08
work_keys_str_mv	AT yingchaoli mesenchymalstemcellderivedexosomesprotecttrabecularmeshworkfromoxidativestress AT juanzheng mesenchymalstemcellderivedexosomesprotecttrabecularmeshworkfromoxidativestress AT xiziwang mesenchymalstemcellderivedexosomesprotecttrabecularmeshworkfromoxidativestress AT xinwang mesenchymalstemcellderivedexosomesprotecttrabecularmeshworkfromoxidativestress AT wenjingliu mesenchymalstemcellderivedexosomesprotecttrabecularmeshworkfromoxidativestress AT jianlugao mesenchymalstemcellderivedexosomesprotecttrabecularmeshworkfromoxidativestress
_version_	1718384059093090304

Mesenchymal stem cell-derived exosomes protect trabecular meshwork from oxidative stress

Ejemplares similares