Method-Related Impacts on Campylobacter coli Recovery From Sampling Materials And Meat

A defined Campylobacter coli (C. coli) suspension was inoculated on sterile sampling materials (cotton bud, polyester bud, cellulose sponge) and pieces of lamb meat. Various combinations of diluents (phosphate buffer saline ± Tween®80) and sampling methods (direct homogenization, simulating the exci...

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Autores principales:	Lazou Thomai, Iossifidou Eleni, Dovas Chrysostomos
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2021
Materias:	campylobacter microbiological sampling bacterial recovery lamb meat Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/776c4890c1674764be0151953288d7ee
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spelling	oai:doaj.org-article:776c4890c1674764be0151953288d7ee2021-11-17T21:27:54ZMethod-Related Impacts on Campylobacter coli Recovery From Sampling Materials And Meat1820-744810.2478/acve-2021-0017https://doaj.org/article/776c4890c1674764be0151953288d7ee2021-06-01T00:00:00Zhttps://doi.org/10.2478/acve-2021-0017https://doaj.org/toc/1820-7448A defined Campylobacter coli (C. coli) suspension was inoculated on sterile sampling materials (cotton bud, polyester bud, cellulose sponge) and pieces of lamb meat. Various combinations of diluents (phosphate buffer saline ± Tween®80) and sampling methods (direct homogenization, simulating the excision method for meat, and swabbing) were investigated for the recovery (detachment) of C. coli cells from the inoculated samples. The obtained C. coli bacteria, as quantified by real-time PCR with respect to the dilution factors and the initial inoculum, were used for the calculation of the recovery (%) per sampling material and method. Regarding artificially inoculated sampling materials, the lowest recovery was observed for cotton buds (2.8%) and the highest for cellulose sponge (28.9%), and the differences between the obtained results were statistically significant (P < 0.05). As regards lamb meat, the lowest recovery was observed for swabbing with cotton buds (3.2%) and the highest for direct homogenization (10.7%). The results indicate an overall low rate of bacterial recovery from contaminated samples, with cellulose sponges and polyester buds being significantly superior to cotton buds, and direct homogenization of meat with diluent better than swabbing. The type of sampling materials and methods applied for the quantification of C. coli entails a key impact on determining the actual contamination of the examined samples.Lazou ThomaiIossifidou EleniDovas ChrysostomosSciendoarticlecampylobactermicrobiological samplingbacterial recoverylamb meatVeterinary medicineSF600-1100ENActa Veterinaria, Vol 71, Iss 2, Pp 198-210 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	campylobacter microbiological sampling bacterial recovery lamb meat Veterinary medicine SF600-1100
spellingShingle	campylobacter microbiological sampling bacterial recovery lamb meat Veterinary medicine SF600-1100 Lazou Thomai Iossifidou Eleni Dovas Chrysostomos Method-Related Impacts on Campylobacter coli Recovery From Sampling Materials And Meat
description	A defined Campylobacter coli (C. coli) suspension was inoculated on sterile sampling materials (cotton bud, polyester bud, cellulose sponge) and pieces of lamb meat. Various combinations of diluents (phosphate buffer saline ± Tween®80) and sampling methods (direct homogenization, simulating the excision method for meat, and swabbing) were investigated for the recovery (detachment) of C. coli cells from the inoculated samples. The obtained C. coli bacteria, as quantified by real-time PCR with respect to the dilution factors and the initial inoculum, were used for the calculation of the recovery (%) per sampling material and method. Regarding artificially inoculated sampling materials, the lowest recovery was observed for cotton buds (2.8%) and the highest for cellulose sponge (28.9%), and the differences between the obtained results were statistically significant (P < 0.05). As regards lamb meat, the lowest recovery was observed for swabbing with cotton buds (3.2%) and the highest for direct homogenization (10.7%). The results indicate an overall low rate of bacterial recovery from contaminated samples, with cellulose sponges and polyester buds being significantly superior to cotton buds, and direct homogenization of meat with diluent better than swabbing. The type of sampling materials and methods applied for the quantification of C. coli entails a key impact on determining the actual contamination of the examined samples.
format	article
author	Lazou Thomai Iossifidou Eleni Dovas Chrysostomos
author_facet	Lazou Thomai Iossifidou Eleni Dovas Chrysostomos
author_sort	Lazou Thomai
title	Method-Related Impacts on Campylobacter coli Recovery From Sampling Materials And Meat
title_short	Method-Related Impacts on Campylobacter coli Recovery From Sampling Materials And Meat
title_full	Method-Related Impacts on Campylobacter coli Recovery From Sampling Materials And Meat
title_fullStr	Method-Related Impacts on Campylobacter coli Recovery From Sampling Materials And Meat
title_full_unstemmed	Method-Related Impacts on Campylobacter coli Recovery From Sampling Materials And Meat
title_sort	method-related impacts on campylobacter coli recovery from sampling materials and meat
publisher	Sciendo
publishDate	2021
url	https://doaj.org/article/776c4890c1674764be0151953288d7ee
work_keys_str_mv	AT lazouthomai methodrelatedimpactsoncampylobactercolirecoveryfromsamplingmaterialsandmeat AT iossifidoueleni methodrelatedimpactsoncampylobactercolirecoveryfromsamplingmaterialsandmeat AT dovaschrysostomos methodrelatedimpactsoncampylobactercolirecoveryfromsamplingmaterialsandmeat
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Method-Related Impacts on Campylobacter coli Recovery From Sampling Materials And Meat

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