Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA

Cortisol is the main glucocorticoid hormone promoting compensatory metabolic responses of stress in teleosts. This hormone acts through genomic and membrane-initiated actions to exert its functions inside the cell. Experimental approaches, using exogenous cortisol administration, confirm the role of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jorge Aedo, Daniela Aravena-Canales, Ignacio Ruiz-Jarabo, Ricardo Oyarzún, Alfredo Molina, Gonzalo Martínez-Rodríguez, Juan Antonio Valdés, Juan Miguel Mancera
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cortisol energetic metabolism fish glucocorticoids <i>Sparus aurata</i> stress response Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/776cb598aebd49808d62eee471476c99
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:776cb598aebd49808d62eee471476c99
record_format	dspace
spelling	oai:doaj.org-article:776cb598aebd49808d62eee471476c992021-11-25T16:21:18ZDifferential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA10.3390/ani111133102076-2615https://doaj.org/article/776cb598aebd49808d62eee471476c992021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3310https://doaj.org/toc/2076-2615Cortisol is the main glucocorticoid hormone promoting compensatory metabolic responses of stress in teleosts. This hormone acts through genomic and membrane-initiated actions to exert its functions inside the cell. Experimental approaches, using exogenous cortisol administration, confirm the role of this hormone during short (minutes to hours)- and long-term (days to weeks) responses to stress. The role of membrane-initiated cortisol signaling during long-term responses has been recently explored. In this study, <i>Sparus aurata</i> were intraperitoneally injected with coconut oil alone or coconut oil containing cortisol, cortisol-BSA, or BSA. After 3 days of treatment, plasma, liver, and skeletal muscle were extracted. Plasma cortisol, as well as metabolic indicators in the plasma and tissues collected, and metabolism-related gene expression, were measured. Our results showed that artificially increased plasma cortisol levels in <i>S. aurata</i> enhanced plasma glucose and triacylglycerols values as well as hepatic substrate energy mobilization. Additionally, cortisol stimulated hepatic carbohydrates metabolism, as seen by the increased expression of metabolism-related genes. All of these responses, observed in cortisol-administered fish, were not detected by replicating the same protocol and instead using cortisol-BSA, which exclusively induces membrane-initiated effects. Therefore, we suggest that after three days of cortisol administration, only genomic actions are involved in the metabolic responses in <i>S. aurata</i>.Jorge AedoDaniela Aravena-CanalesIgnacio Ruiz-JaraboRicardo OyarzúnAlfredo MolinaGonzalo Martínez-RodríguezJuan Antonio ValdésJuan Miguel ManceraMDPI AGarticlecortisolenergetic metabolismfishglucocorticoids<i>Sparus aurata</i>stress responseVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3310, p 3310 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cortisol energetic metabolism fish glucocorticoids <i>Sparus aurata</i> stress response Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	cortisol energetic metabolism fish glucocorticoids <i>Sparus aurata</i> stress response Veterinary medicine SF600-1100 Zoology QL1-991 Jorge Aedo Daniela Aravena-Canales Ignacio Ruiz-Jarabo Ricardo Oyarzún Alfredo Molina Gonzalo Martínez-Rodríguez Juan Antonio Valdés Juan Miguel Mancera Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA
description	Cortisol is the main glucocorticoid hormone promoting compensatory metabolic responses of stress in teleosts. This hormone acts through genomic and membrane-initiated actions to exert its functions inside the cell. Experimental approaches, using exogenous cortisol administration, confirm the role of this hormone during short (minutes to hours)- and long-term (days to weeks) responses to stress. The role of membrane-initiated cortisol signaling during long-term responses has been recently explored. In this study, <i>Sparus aurata</i> were intraperitoneally injected with coconut oil alone or coconut oil containing cortisol, cortisol-BSA, or BSA. After 3 days of treatment, plasma, liver, and skeletal muscle were extracted. Plasma cortisol, as well as metabolic indicators in the plasma and tissues collected, and metabolism-related gene expression, were measured. Our results showed that artificially increased plasma cortisol levels in <i>S. aurata</i> enhanced plasma glucose and triacylglycerols values as well as hepatic substrate energy mobilization. Additionally, cortisol stimulated hepatic carbohydrates metabolism, as seen by the increased expression of metabolism-related genes. All of these responses, observed in cortisol-administered fish, were not detected by replicating the same protocol and instead using cortisol-BSA, which exclusively induces membrane-initiated effects. Therefore, we suggest that after three days of cortisol administration, only genomic actions are involved in the metabolic responses in <i>S. aurata</i>.
format	article
author	Jorge Aedo Daniela Aravena-Canales Ignacio Ruiz-Jarabo Ricardo Oyarzún Alfredo Molina Gonzalo Martínez-Rodríguez Juan Antonio Valdés Juan Miguel Mancera
author_facet	Jorge Aedo Daniela Aravena-Canales Ignacio Ruiz-Jarabo Ricardo Oyarzún Alfredo Molina Gonzalo Martínez-Rodríguez Juan Antonio Valdés Juan Miguel Mancera
author_sort	Jorge Aedo
title	Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA
title_short	Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA
title_full	Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA
title_fullStr	Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA
title_full_unstemmed	Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA
title_sort	differential metabolic and transcriptional responses of gilthead seabream (<i>sparus aurata</i>) administered with cortisol or cortisol-bsa
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/776cb598aebd49808d62eee471476c99
work_keys_str_mv	AT jorgeaedo differentialmetabolicandtranscriptionalresponsesofgiltheadseabreamisparusaurataiadministeredwithcortisolorcortisolbsa AT danielaaravenacanales differentialmetabolicandtranscriptionalresponsesofgiltheadseabreamisparusaurataiadministeredwithcortisolorcortisolbsa AT ignacioruizjarabo differentialmetabolicandtranscriptionalresponsesofgiltheadseabreamisparusaurataiadministeredwithcortisolorcortisolbsa AT ricardooyarzun differentialmetabolicandtranscriptionalresponsesofgiltheadseabreamisparusaurataiadministeredwithcortisolorcortisolbsa AT alfredomolina differentialmetabolicandtranscriptionalresponsesofgiltheadseabreamisparusaurataiadministeredwithcortisolorcortisolbsa AT gonzalomartinezrodriguez differentialmetabolicandtranscriptionalresponsesofgiltheadseabreamisparusaurataiadministeredwithcortisolorcortisolbsa AT juanantoniovaldes differentialmetabolicandtranscriptionalresponsesofgiltheadseabreamisparusaurataiadministeredwithcortisolorcortisolbsa AT juanmiguelmancera differentialmetabolicandtranscriptionalresponsesofgiltheadseabreamisparusaurataiadministeredwithcortisolorcortisolbsa
_version_	1718413207339532288

Differential Metabolic and Transcriptional Responses of Gilthead Seabream (<i>Sparus aurata</i>) Administered with Cortisol or Cortisol-BSA

Ejemplares similares