Using fecal immunochemical tubes for the analysis of the gut microbiome has the potential to improve colorectal cancer screening

Abstract Colorectal cancer (CRC) is a challenging public health problem which successful treatment depends on the stage at diagnosis. Recently, CRC-specific microbiome signatures have been proposed as a marker for CRC detection. Since many countries have initiated CRC screening programs, it would be...

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Autores principales:	Kertu Liis Krigul, Oliver Aasmets, Kreete Lüll, Tõnis Org, Elin Org
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/776eb169a535478a86815527a1a134de
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spelling	oai:doaj.org-article:776eb169a535478a86815527a1a134de2021-12-02T18:51:07ZUsing fecal immunochemical tubes for the analysis of the gut microbiome has the potential to improve colorectal cancer screening10.1038/s41598-021-99046-w2045-2322https://doaj.org/article/776eb169a535478a86815527a1a134de2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99046-whttps://doaj.org/toc/2045-2322Abstract Colorectal cancer (CRC) is a challenging public health problem which successful treatment depends on the stage at diagnosis. Recently, CRC-specific microbiome signatures have been proposed as a marker for CRC detection. Since many countries have initiated CRC screening programs, it would be useful to analyze the microbiome in the samples collected in fecal immunochemical test (FIT) tubes for fecal occult blood testing. Therefore, we investigated the impact of FIT tubes and stabilization buffer on the microbial community structure evaluated in stool samples from 30 volunteers and compared the detected communities to those of fresh-frozen samples, highlighting previously published cancer-specific communities. Altogether, 214 samples were analyzed by 16S rRNA gene sequencing, including positive and negative controls. Our results indicated that the variation between individuals was greater than the differences introduced by the collection strategy. The vast majority of the genera were stable for up to 7 days. None of the changes observed between fresh-frozen samples and FIT tube specimens were related to previously identified CRC-specific bacteria. Overall, we show that FIT tubes can be used for profiling the microbiota in CRC screening programs. This circumvents the need to collect additional samples and can possibly improve the sensitivity of CRC detection.Kertu Liis KrigulOliver AasmetsKreete LüllTõnis OrgElin OrgNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kertu Liis Krigul Oliver Aasmets Kreete Lüll Tõnis Org Elin Org Using fecal immunochemical tubes for the analysis of the gut microbiome has the potential to improve colorectal cancer screening
description	Abstract Colorectal cancer (CRC) is a challenging public health problem which successful treatment depends on the stage at diagnosis. Recently, CRC-specific microbiome signatures have been proposed as a marker for CRC detection. Since many countries have initiated CRC screening programs, it would be useful to analyze the microbiome in the samples collected in fecal immunochemical test (FIT) tubes for fecal occult blood testing. Therefore, we investigated the impact of FIT tubes and stabilization buffer on the microbial community structure evaluated in stool samples from 30 volunteers and compared the detected communities to those of fresh-frozen samples, highlighting previously published cancer-specific communities. Altogether, 214 samples were analyzed by 16S rRNA gene sequencing, including positive and negative controls. Our results indicated that the variation between individuals was greater than the differences introduced by the collection strategy. The vast majority of the genera were stable for up to 7 days. None of the changes observed between fresh-frozen samples and FIT tube specimens were related to previously identified CRC-specific bacteria. Overall, we show that FIT tubes can be used for profiling the microbiota in CRC screening programs. This circumvents the need to collect additional samples and can possibly improve the sensitivity of CRC detection.
format	article
author	Kertu Liis Krigul Oliver Aasmets Kreete Lüll Tõnis Org Elin Org
author_facet	Kertu Liis Krigul Oliver Aasmets Kreete Lüll Tõnis Org Elin Org
author_sort	Kertu Liis Krigul
title	Using fecal immunochemical tubes for the analysis of the gut microbiome has the potential to improve colorectal cancer screening
title_short	Using fecal immunochemical tubes for the analysis of the gut microbiome has the potential to improve colorectal cancer screening
title_full	Using fecal immunochemical tubes for the analysis of the gut microbiome has the potential to improve colorectal cancer screening
title_fullStr	Using fecal immunochemical tubes for the analysis of the gut microbiome has the potential to improve colorectal cancer screening
title_full_unstemmed	Using fecal immunochemical tubes for the analysis of the gut microbiome has the potential to improve colorectal cancer screening
title_sort	using fecal immunochemical tubes for the analysis of the gut microbiome has the potential to improve colorectal cancer screening
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/776eb169a535478a86815527a1a134de
work_keys_str_mv	AT kertuliiskrigul usingfecalimmunochemicaltubesfortheanalysisofthegutmicrobiomehasthepotentialtoimprovecolorectalcancerscreening AT oliveraasmets usingfecalimmunochemicaltubesfortheanalysisofthegutmicrobiomehasthepotentialtoimprovecolorectalcancerscreening AT kreetelull usingfecalimmunochemicaltubesfortheanalysisofthegutmicrobiomehasthepotentialtoimprovecolorectalcancerscreening AT tonisorg usingfecalimmunochemicaltubesfortheanalysisofthegutmicrobiomehasthepotentialtoimprovecolorectalcancerscreening AT elinorg usingfecalimmunochemicaltubesfortheanalysisofthegutmicrobiomehasthepotentialtoimprovecolorectalcancerscreening
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Using fecal immunochemical tubes for the analysis of the gut microbiome has the potential to improve colorectal cancer screening

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