Probiotics protect mice from CoCrMo particles-induced osteolysis

Zhenheng Wang,* Kaiwen Xue,* Maosheng Bai, Zhantao Deng, Jingjing Gan, Gang Zhou, Hongbo Qian,* Nirong Bao, Jianning Zhao Department of Orthopaedics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, People’s Republic of China *These authors contributed equally to this w...

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Autores principales:	Wang Z, Xue K, Bai M, Deng Z, Gan J, Zhou G, Qian H, Bao N, Zhao J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Wear particles gut microbiota nanotoxicity macrophage polarization inflammatory cytokines aseptic loosening. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/777a006431794b19a4a976e4a70040de
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spelling	oai:doaj.org-article:777a006431794b19a4a976e4a70040de2021-12-02T02:42:21ZProbiotics protect mice from CoCrMo particles-induced osteolysis1178-2013https://doaj.org/article/777a006431794b19a4a976e4a70040de2017-07-01T00:00:00Zhttps://www.dovepress.com/probiotics-protect-mice-from-cocrmo-particles-induced-osteolysis-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhenheng Wang,* Kaiwen Xue,* Maosheng Bai, Zhantao Deng, Jingjing Gan, Gang Zhou, Hongbo Qian,* Nirong Bao, Jianning Zhao Department of Orthopaedics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, People’s Republic of China *These authors contributed equally to this work Abstract: Wear particle-induced inflammatory osteolysis is the primary cause of aseptic loosening, which is the most common reason for total hip arthroplasty (THA) failure in the med- and long term. Recent studies have suggested an important role of gut microbiota (GM) in modulating the host metabolism and immune system, leading to alterations in bone mass. Probiotic bacteria administered in adequate amounts can alter the composition of GM and confer health benefits to the host. Given the inflammatory osteolysis that occurs in wear debris-induced prosthesis loosening, we examined whether the probiotic Lactobacillus casei could reduce osteolysis in a mouse calvarial resorption model. In this study, L. casei markedly protected mice from CoCrMo particles (CoPs)-induced osteolysis. Osteoclast gene markers and the number of osteoclasts were significantly decreased in L. casei-treated mice. Probiotic treatment decreased the M1-like macrophage phenotype indicated by downregulation of tumor necrosis factor α (TNF-α), interleukin (IL)-6 and inducible nitric oxide synthase (iNOS) and increased the M2-like macrophage phenotype indicated by upregulation of IL-4, IL-10 and arginase. Collectively, these results indicated that the L. casei treatment modulated the immune status and suppressed wear particle-induced osteolysis in vivo. Thus, probiotic treatment may represent a potential preventive and therapeutic approach to reduced wear debris-induced osteolysis. Keywords: wear particles, gut microbiota, nanotoxicity, macrophage polarization, inflammatory cytokines, aseptic looseningWang ZXue KBai MDeng ZGan JZhou GQian HBao NZhao JDove Medical PressarticleWear particlesgut microbiotananotoxicitymacrophage polarizationinflammatory cytokinesaseptic loosening.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 5387-5397 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Wear particles gut microbiota nanotoxicity macrophage polarization inflammatory cytokines aseptic loosening. Medicine (General) R5-920
spellingShingle	Wear particles gut microbiota nanotoxicity macrophage polarization inflammatory cytokines aseptic loosening. Medicine (General) R5-920 Wang Z Xue K Bai M Deng Z Gan J Zhou G Qian H Bao N Zhao J Probiotics protect mice from CoCrMo particles-induced osteolysis
description	Zhenheng Wang,* Kaiwen Xue,* Maosheng Bai, Zhantao Deng, Jingjing Gan, Gang Zhou, Hongbo Qian,* Nirong Bao, Jianning Zhao Department of Orthopaedics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, People’s Republic of China *These authors contributed equally to this work Abstract: Wear particle-induced inflammatory osteolysis is the primary cause of aseptic loosening, which is the most common reason for total hip arthroplasty (THA) failure in the med- and long term. Recent studies have suggested an important role of gut microbiota (GM) in modulating the host metabolism and immune system, leading to alterations in bone mass. Probiotic bacteria administered in adequate amounts can alter the composition of GM and confer health benefits to the host. Given the inflammatory osteolysis that occurs in wear debris-induced prosthesis loosening, we examined whether the probiotic Lactobacillus casei could reduce osteolysis in a mouse calvarial resorption model. In this study, L. casei markedly protected mice from CoCrMo particles (CoPs)-induced osteolysis. Osteoclast gene markers and the number of osteoclasts were significantly decreased in L. casei-treated mice. Probiotic treatment decreased the M1-like macrophage phenotype indicated by downregulation of tumor necrosis factor α (TNF-α), interleukin (IL)-6 and inducible nitric oxide synthase (iNOS) and increased the M2-like macrophage phenotype indicated by upregulation of IL-4, IL-10 and arginase. Collectively, these results indicated that the L. casei treatment modulated the immune status and suppressed wear particle-induced osteolysis in vivo. Thus, probiotic treatment may represent a potential preventive and therapeutic approach to reduced wear debris-induced osteolysis. Keywords: wear particles, gut microbiota, nanotoxicity, macrophage polarization, inflammatory cytokines, aseptic loosening
format	article
author	Wang Z Xue K Bai M Deng Z Gan J Zhou G Qian H Bao N Zhao J
author_facet	Wang Z Xue K Bai M Deng Z Gan J Zhou G Qian H Bao N Zhao J
author_sort	Wang Z
title	Probiotics protect mice from CoCrMo particles-induced osteolysis
title_short	Probiotics protect mice from CoCrMo particles-induced osteolysis
title_full	Probiotics protect mice from CoCrMo particles-induced osteolysis
title_fullStr	Probiotics protect mice from CoCrMo particles-induced osteolysis
title_full_unstemmed	Probiotics protect mice from CoCrMo particles-induced osteolysis
title_sort	probiotics protect mice from cocrmo particles-induced osteolysis
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/777a006431794b19a4a976e4a70040de
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_version_	1718402226978816000

Probiotics protect mice from CoCrMo particles-induced osteolysis

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