Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil

Abstract As an approach to the exploration of cold-active enzymes, in this study, we isolated a cold-active protease produced by psychrotrophic bacteria from glacial soils of Thajwas Glacier, Himalayas. The isolated strain BO1, identified as Bacillus pumilus, grew well within a temperature range of...

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Autores principales:	Saleem Farooq, Ruqeya Nazir, Shabir Ahmad Ganai, Bashir Ahmad Ganai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/777b739b748d4c538e9517776a8c0ced
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spelling	oai:doaj.org-article:777b739b748d4c538e9517776a8c0ced2021-12-02T17:39:57ZIsolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil10.1038/s41598-021-92197-w2045-2322https://doaj.org/article/777b739b748d4c538e9517776a8c0ced2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92197-whttps://doaj.org/toc/2045-2322Abstract As an approach to the exploration of cold-active enzymes, in this study, we isolated a cold-active protease produced by psychrotrophic bacteria from glacial soils of Thajwas Glacier, Himalayas. The isolated strain BO1, identified as Bacillus pumilus, grew well within a temperature range of 4–30 °C. After its qualitative and quantitative screening, the cold-active protease (Apr-BO1) was purified. The Apr-BO1 had a molecular mass of 38 kDa and showed maximum (37.02 U/mg) specific activity at 20 °C, with casein as substrate. It was stable and active between the temperature range of 5–35 °C and pH 6.0–12.0, with an optimum temperature of 20 °C at pH 9.0. The Apr-BO1 had low Km value of 1.0 mg/ml and V max 10.0 µmol/ml/min. Moreover, it displayed better tolerance to organic solvents, surfactants, metal ions and reducing agents than most alkaline proteases. The results exhibited that it effectively removed the stains even in a cold wash and could be considered a decent detergent additive. Furthermore, through protein modelling, the structure of this protease was generated from template, subtilisin E of Bacillus subtilis (PDB ID: 3WHI), and different methods checked its quality. For the first time, this study reported the protein sequence for psychrotrophic Apr-BO1 and brought forth its novelty among other cold-active proteases.Saleem FarooqRuqeya NazirShabir Ahmad GanaiBashir Ahmad GanaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saleem Farooq Ruqeya Nazir Shabir Ahmad Ganai Bashir Ahmad Ganai Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil
description	Abstract As an approach to the exploration of cold-active enzymes, in this study, we isolated a cold-active protease produced by psychrotrophic bacteria from glacial soils of Thajwas Glacier, Himalayas. The isolated strain BO1, identified as Bacillus pumilus, grew well within a temperature range of 4–30 °C. After its qualitative and quantitative screening, the cold-active protease (Apr-BO1) was purified. The Apr-BO1 had a molecular mass of 38 kDa and showed maximum (37.02 U/mg) specific activity at 20 °C, with casein as substrate. It was stable and active between the temperature range of 5–35 °C and pH 6.0–12.0, with an optimum temperature of 20 °C at pH 9.0. The Apr-BO1 had low Km value of 1.0 mg/ml and V max 10.0 µmol/ml/min. Moreover, it displayed better tolerance to organic solvents, surfactants, metal ions and reducing agents than most alkaline proteases. The results exhibited that it effectively removed the stains even in a cold wash and could be considered a decent detergent additive. Furthermore, through protein modelling, the structure of this protease was generated from template, subtilisin E of Bacillus subtilis (PDB ID: 3WHI), and different methods checked its quality. For the first time, this study reported the protein sequence for psychrotrophic Apr-BO1 and brought forth its novelty among other cold-active proteases.
format	article
author	Saleem Farooq Ruqeya Nazir Shabir Ahmad Ganai Bashir Ahmad Ganai
author_facet	Saleem Farooq Ruqeya Nazir Shabir Ahmad Ganai Bashir Ahmad Ganai
author_sort	Saleem Farooq
title	Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil
title_short	Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil
title_full	Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil
title_fullStr	Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil
title_full_unstemmed	Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil
title_sort	isolation and characterization of a new cold-active protease from psychrotrophic bacteria of western himalayan glacial soil
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/777b739b748d4c538e9517776a8c0ced
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Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil

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