Imaging gold nanoparticles in mouse liver by laser ablation inductively coupled plasma mass spectrometry

Abstract Imaging the size distribution of metal nanoparticles (NPs) in a tissue has important implications in terms of evaluating NP toxicity. Microscopy techniques used to image tissue NPs are limited by complicated sample preparation or poor resolution. In this study, we developed a laser ablation...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Qing Li, Zheng Wang, Jiamei Mo, Guoxia Zhang, Yirui Chen, Chuchu Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/777b8472a117437dad8363dd7f561e92
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:777b8472a117437dad8363dd7f561e92
record_format	dspace
spelling	oai:doaj.org-article:777b8472a117437dad8363dd7f561e922021-12-02T16:06:20ZImaging gold nanoparticles in mouse liver by laser ablation inductively coupled plasma mass spectrometry10.1038/s41598-017-03275-x2045-2322https://doaj.org/article/777b8472a117437dad8363dd7f561e922017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03275-xhttps://doaj.org/toc/2045-2322Abstract Imaging the size distribution of metal nanoparticles (NPs) in a tissue has important implications in terms of evaluating NP toxicity. Microscopy techniques used to image tissue NPs are limited by complicated sample preparation or poor resolution. In this study, we developed a laser ablation (LA) system coupled to single-particle inductively coupled plasma mass spectrometry (SP-ICP-MS) for quantitative imaging of gold (G)NPs in tissue samples. In this system, GNPs were ablated but did not disintegrate and integrate under optimised operation conditions, which were verified by characterising LA particles by scanning electron microscopy. The feasibility of imaging size distributions in tissue was validated using reference GNPs 60 and 80 nm in size on matrix-matched kidney. A transport efficiency of 6.07% was obtained by LA-SP-ICP-MS under optimal conditions. We used this system to image 80-nm GNPs in mouse liver and the size distribution thus obtained was in accordance with that determined by nebuliser SP-ICP-MS. The images revealed that 80-nm GNPs mainly accumulate in the liver and did not obviously aggregate. Our results demonstrate that LA-SP-ICP-MS is an effective tool for evaluating the size distribution of metal NPs in tissue.Qing LiZheng WangJiamei MoGuoxia ZhangYirui ChenChuchu HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qing Li Zheng Wang Jiamei Mo Guoxia Zhang Yirui Chen Chuchu Huang Imaging gold nanoparticles in mouse liver by laser ablation inductively coupled plasma mass spectrometry
description	Abstract Imaging the size distribution of metal nanoparticles (NPs) in a tissue has important implications in terms of evaluating NP toxicity. Microscopy techniques used to image tissue NPs are limited by complicated sample preparation or poor resolution. In this study, we developed a laser ablation (LA) system coupled to single-particle inductively coupled plasma mass spectrometry (SP-ICP-MS) for quantitative imaging of gold (G)NPs in tissue samples. In this system, GNPs were ablated but did not disintegrate and integrate under optimised operation conditions, which were verified by characterising LA particles by scanning electron microscopy. The feasibility of imaging size distributions in tissue was validated using reference GNPs 60 and 80 nm in size on matrix-matched kidney. A transport efficiency of 6.07% was obtained by LA-SP-ICP-MS under optimal conditions. We used this system to image 80-nm GNPs in mouse liver and the size distribution thus obtained was in accordance with that determined by nebuliser SP-ICP-MS. The images revealed that 80-nm GNPs mainly accumulate in the liver and did not obviously aggregate. Our results demonstrate that LA-SP-ICP-MS is an effective tool for evaluating the size distribution of metal NPs in tissue.
format	article
author	Qing Li Zheng Wang Jiamei Mo Guoxia Zhang Yirui Chen Chuchu Huang
author_facet	Qing Li Zheng Wang Jiamei Mo Guoxia Zhang Yirui Chen Chuchu Huang
author_sort	Qing Li
title	Imaging gold nanoparticles in mouse liver by laser ablation inductively coupled plasma mass spectrometry
title_short	Imaging gold nanoparticles in mouse liver by laser ablation inductively coupled plasma mass spectrometry
title_full	Imaging gold nanoparticles in mouse liver by laser ablation inductively coupled plasma mass spectrometry
title_fullStr	Imaging gold nanoparticles in mouse liver by laser ablation inductively coupled plasma mass spectrometry
title_full_unstemmed	Imaging gold nanoparticles in mouse liver by laser ablation inductively coupled plasma mass spectrometry
title_sort	imaging gold nanoparticles in mouse liver by laser ablation inductively coupled plasma mass spectrometry
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/777b8472a117437dad8363dd7f561e92
work_keys_str_mv	AT qingli imaginggoldnanoparticlesinmouseliverbylaserablationinductivelycoupledplasmamassspectrometry AT zhengwang imaginggoldnanoparticlesinmouseliverbylaserablationinductivelycoupledplasmamassspectrometry AT jiameimo imaginggoldnanoparticlesinmouseliverbylaserablationinductivelycoupledplasmamassspectrometry AT guoxiazhang imaginggoldnanoparticlesinmouseliverbylaserablationinductivelycoupledplasmamassspectrometry AT yiruichen imaginggoldnanoparticlesinmouseliverbylaserablationinductivelycoupledplasmamassspectrometry AT chuchuhuang imaginggoldnanoparticlesinmouseliverbylaserablationinductivelycoupledplasmamassspectrometry
_version_	1718385032714780672

Imaging gold nanoparticles in mouse liver by laser ablation inductively coupled plasma mass spectrometry

Ejemplares similares