Effect of Erythromycin on the Intestinal Transport of Cyclosporine

BACKGROUND AND OBJECTIVE: Co-administration of cyclosporine and erythromycin can cause nephrotoxicity which could be a result of interaction in absorption and/or metabolism level. The purpose of this work was to investigate the interaction of erythromycin and cyclosporine in absorption level to eval...

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Autores principales:	P Zakeri Milani, S Damani, Z Islambulchilar, M Mehtari, H Valizadeh
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2009
Materias:	transport erythromycin perfusion efflux pump cyclosporine Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/778346e35d4644f5bc5a546e13615226
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spelling	oai:doaj.org-article:778346e35d4644f5bc5a546e136152262021-11-10T09:02:43ZEffect of Erythromycin on the Intestinal Transport of Cyclosporine1561-41072251-7170https://doaj.org/article/778346e35d4644f5bc5a546e136152262009-06-01T00:00:00Zhttp://jbums.org/article-1-3122-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Co-administration of cyclosporine and erythromycin can cause nephrotoxicity which could be a result of interaction in absorption and/or metabolism level. The purpose of this work was to investigate the interaction of erythromycin and cyclosporine in absorption level to evaluate the effect of erythromycin on cyclosporine absorption quantitatively. The stability of drug at 37°C and its adsorption to plastic syringe and tubes were also investigated.METHODS: 32 Wistar rats weighting between 200-300 g were used in the present study. They were divided into 4 test groups and 4 control groups. A jejunal segment of anaesthetized rat was cannulated and perfused by concentrations of 20, 15, 10 and 5 micromolar of drug in the presence and absence of 150 micromolar erythromycin by flow rate of 0.2 ml/min. Samples were obtained up to 90 min. Drug concentrations were assayed in different time points. The drug effective permeability values were calculated for its different concentrations in different experiments. A reverse-phase HPLC method was used for analysis of all samples.FINDINGS: The cyclosporine effective permeability values were increased from 22.1-33.4 (×10-6) cm/sec in control group to 37.2-53.4 (×10-6) cm/sec in test group that this difference was significant (p<0.05). Moreover cyclosporine was stable and only 4-5% loss of drug was observed at the end of experiment. However there was a significant adsorption of drug to syringe and tubing.CONCLUSION: Based on obtained results cyclosporine is a low solubility and high permeability drug. At least some part of clinical interaction between cyclosporine and erythromycin is due to interaction in absorption level.P Zakeri Milani,S Damani,Z IslambulchilarM Mehtari,H ValizadehBabol University of Medical Sciencesarticletransporterythromycinperfusionefflux pumpcyclosporineMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 11, Iss 2, Pp 7-15 (2009)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	transport erythromycin perfusion efflux pump cyclosporine Medicine R Medicine (General) R5-920
spellingShingle	transport erythromycin perfusion efflux pump cyclosporine Medicine R Medicine (General) R5-920 P Zakeri Milani, S Damani, Z Islambulchilar M Mehtari, H Valizadeh Effect of Erythromycin on the Intestinal Transport of Cyclosporine
description	BACKGROUND AND OBJECTIVE: Co-administration of cyclosporine and erythromycin can cause nephrotoxicity which could be a result of interaction in absorption and/or metabolism level. The purpose of this work was to investigate the interaction of erythromycin and cyclosporine in absorption level to evaluate the effect of erythromycin on cyclosporine absorption quantitatively. The stability of drug at 37°C and its adsorption to plastic syringe and tubes were also investigated.METHODS: 32 Wistar rats weighting between 200-300 g were used in the present study. They were divided into 4 test groups and 4 control groups. A jejunal segment of anaesthetized rat was cannulated and perfused by concentrations of 20, 15, 10 and 5 micromolar of drug in the presence and absence of 150 micromolar erythromycin by flow rate of 0.2 ml/min. Samples were obtained up to 90 min. Drug concentrations were assayed in different time points. The drug effective permeability values were calculated for its different concentrations in different experiments. A reverse-phase HPLC method was used for analysis of all samples.FINDINGS: The cyclosporine effective permeability values were increased from 22.1-33.4 (×10-6) cm/sec in control group to 37.2-53.4 (×10-6) cm/sec in test group that this difference was significant (p<0.05). Moreover cyclosporine was stable and only 4-5% loss of drug was observed at the end of experiment. However there was a significant adsorption of drug to syringe and tubing.CONCLUSION: Based on obtained results cyclosporine is a low solubility and high permeability drug. At least some part of clinical interaction between cyclosporine and erythromycin is due to interaction in absorption level.
format	article
author	P Zakeri Milani, S Damani, Z Islambulchilar M Mehtari, H Valizadeh
author_facet	P Zakeri Milani, S Damani, Z Islambulchilar M Mehtari, H Valizadeh
author_sort	P Zakeri Milani,
title	Effect of Erythromycin on the Intestinal Transport of Cyclosporine
title_short	Effect of Erythromycin on the Intestinal Transport of Cyclosporine
title_full	Effect of Erythromycin on the Intestinal Transport of Cyclosporine
title_fullStr	Effect of Erythromycin on the Intestinal Transport of Cyclosporine
title_full_unstemmed	Effect of Erythromycin on the Intestinal Transport of Cyclosporine
title_sort	effect of erythromycin on the intestinal transport of cyclosporine
publisher	Babol University of Medical Sciences
publishDate	2009
url	https://doaj.org/article/778346e35d4644f5bc5a546e13615226
work_keys_str_mv	AT pzakerimilani effectoferythromycinontheintestinaltransportofcyclosporine AT sdamani effectoferythromycinontheintestinaltransportofcyclosporine AT zislambulchilar effectoferythromycinontheintestinaltransportofcyclosporine AT mmehtari effectoferythromycinontheintestinaltransportofcyclosporine AT hvalizadeh effectoferythromycinontheintestinaltransportofcyclosporine
_version_	1718440298438197248

Effect of Erythromycin on the Intestinal Transport of Cyclosporine

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