Expression of VEGF in human conjunctival melanoma analyzed with immunohistochemistry

Satoru Kase, Iku Kikuchi, Susumu Ishida Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan Purpose: The aim of this study was to examine the immunolocalization of VEGF-A and CD34, a marker of endothelial cells, in human co...

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Autores principales:	Kase S, Kikuchi I, Ishida S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	conjunctival melanoma immunohistochemistry microvessels VEGF Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/7783c52ecbf74ec680feb1a8172ee878
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spelling	oai:doaj.org-article:7783c52ecbf74ec680feb1a8172ee8782021-12-02T07:41:46ZExpression of VEGF in human conjunctival melanoma analyzed with immunohistochemistry1177-5483https://doaj.org/article/7783c52ecbf74ec680feb1a8172ee8782018-11-01T00:00:00Zhttps://www.dovepress.com/expression-of-vegf-in-human-conjunctival-melanoma-analyzed-with-immuno-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Satoru Kase, Iku Kikuchi, Susumu Ishida Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan Purpose: The aim of this study was to examine the immunolocalization of VEGF-A and CD34, a marker of endothelial cells, in human conjunctival melanoma.Methods: This study retrospectively analyzed primary conjunctival melanoma patients who underwent surgical resection of the tumor. All excised tissues were fixed with paraformaldehyde and embedded in paraffin, which were then submitted for immunohistochemistry with anti-VEGF and CD34 antibodies.Results: The study sample comprised 4 female and two male melanoma patients. The age of the patients ranged from 64 to 84 (average age, 73) years. Histopathology of the surgically resected tumor tissues demonstrated accumulation of polygonal atypical malignant cells producing melanin. Cytoplasmic immunoreactivity for VEGF was clearly observed in tumor cells of all six tumors. In contrast, CD34-positive endothelial cells were less marked in the melanoma tissues than in the adjacent noncancerous subconjunctival stroma.Conclusion: VEGF immunoreactivity was observed in conjunctival melanoma tissues, in which endothelial cells were hardly observed. These results suggest that although VEGF is expressed, conjunctival melanoma is a hypovascular tumor. Keywords: conjunctival melanoma, immunohistochemistry, microvessels, VEGF Kase SKikuchi IIshida SDove Medical Pressarticleconjunctival melanomaimmunohistochemistrymicrovesselsVEGFOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 12, Pp 2363-2367 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	conjunctival melanoma immunohistochemistry microvessels VEGF Ophthalmology RE1-994
spellingShingle	conjunctival melanoma immunohistochemistry microvessels VEGF Ophthalmology RE1-994 Kase S Kikuchi I Ishida S Expression of VEGF in human conjunctival melanoma analyzed with immunohistochemistry
description	Satoru Kase, Iku Kikuchi, Susumu Ishida Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan Purpose: The aim of this study was to examine the immunolocalization of VEGF-A and CD34, a marker of endothelial cells, in human conjunctival melanoma.Methods: This study retrospectively analyzed primary conjunctival melanoma patients who underwent surgical resection of the tumor. All excised tissues were fixed with paraformaldehyde and embedded in paraffin, which were then submitted for immunohistochemistry with anti-VEGF and CD34 antibodies.Results: The study sample comprised 4 female and two male melanoma patients. The age of the patients ranged from 64 to 84 (average age, 73) years. Histopathology of the surgically resected tumor tissues demonstrated accumulation of polygonal atypical malignant cells producing melanin. Cytoplasmic immunoreactivity for VEGF was clearly observed in tumor cells of all six tumors. In contrast, CD34-positive endothelial cells were less marked in the melanoma tissues than in the adjacent noncancerous subconjunctival stroma.Conclusion: VEGF immunoreactivity was observed in conjunctival melanoma tissues, in which endothelial cells were hardly observed. These results suggest that although VEGF is expressed, conjunctival melanoma is a hypovascular tumor. Keywords: conjunctival melanoma, immunohistochemistry, microvessels, VEGF
format	article
author	Kase S Kikuchi I Ishida S
author_facet	Kase S Kikuchi I Ishida S
author_sort	Kase S
title	Expression of VEGF in human conjunctival melanoma analyzed with immunohistochemistry
title_short	Expression of VEGF in human conjunctival melanoma analyzed with immunohistochemistry
title_full	Expression of VEGF in human conjunctival melanoma analyzed with immunohistochemistry
title_fullStr	Expression of VEGF in human conjunctival melanoma analyzed with immunohistochemistry
title_full_unstemmed	Expression of VEGF in human conjunctival melanoma analyzed with immunohistochemistry
title_sort	expression of vegf in human conjunctival melanoma analyzed with immunohistochemistry
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/7783c52ecbf74ec680feb1a8172ee878
work_keys_str_mv	AT kases expressionofvegfinhumanconjunctivalmelanomaanalyzedwithimmunohistochemistry AT kikuchii expressionofvegfinhumanconjunctivalmelanomaanalyzedwithimmunohistochemistry AT ishidas expressionofvegfinhumanconjunctivalmelanomaanalyzedwithimmunohistochemistry
_version_	1718399230177968128

Expression of VEGF in human conjunctival melanoma analyzed with immunohistochemistry

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