A new approach to assessment of reproductive losses of the first trimester of pregnancy

Background. At present, the role of cytomegalovirus infection in the disruption of acental formation and associated pathological conditions of the fetus is bvious, which are based on oxidative and inflammatory reactions that cause disruption of igration and invasion of trophoblasts, angiogenic modul...

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Autores principales:	N. A. Ishutina, I. A. Andrievskaya, N. G. Prikhodko
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2021
Materias:	pregnancy cytomegalovirus infection hypoxia-inducible factor 1α α-tocopherol docosahexaenoic acid Science Q
Acceso en línea:	https://doaj.org/article/77855a45eb3d4e92a06bffeec7e812dc
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spelling	oai:doaj.org-article:77855a45eb3d4e92a06bffeec7e812dc2021-11-23T06:14:46ZA new approach to assessment of reproductive losses of the first trimester of pregnancy2541-94202587-959610.29413/ABS.2021-6.3.4https://doaj.org/article/77855a45eb3d4e92a06bffeec7e812dc2021-08-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2844https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. At present, the role of cytomegalovirus infection in the disruption of acental formation and associated pathological conditions of the fetus is bvious, which are based on oxidative and inflammatory reactions that cause disruption of igration and invasion of trophoblasts, angiogenic modulation of placental and uterine vessels.Objective: To analyze the quantitative indicators of hypoxia-inducible factor 1α, α-tocopherol and docosahexaenoic acid in the peripheral blood in comparison with morphological changes in the placenta and uterine mucosa in seropositive women with spontaneous abortion associated with reactivation of ytomegaloviral infection.Methods: Studies were carried out at 7-9 weeks of gestation. 67 observations were analyzed, including 35 seropositive women in the first trimester with spontaneous abortion associated with reactivation of cytomegaloviral infection and 32 healthy seronerative women with voluntary termination of pregnancy. Stabilized blood from vein, epithelium of the inner surface of the cheek, cervical canal, urine, villous chorion, and uterine mucosa were used as the test material. Enzyme immunoassay was used to detect serological markers of cytomegaloviral infection (CMV-IgG/IgM antibodies, CMV-IgG avidity), hypoxia-induced factor 1α, α-tocopherol – by fluorometric method, PCR (polymerase chain reaction) in real time – DNA CMV, the method of gas-liquid chromatography – docosahexaenoic acid, histological methods – the general plan of the structure of the chorionic villus and the mucous membrane of the uterus.Results: In seropositive women with spontaneous abortion associated with reactivation of cytomegaloviral infection, the average of hypoxia-induced factor 1α was 1.54 times higher, α-tocopherol and docosahexaenoic acid were 1.71 times lower and 1.40 times lower (p < 0.001, respectively) than in the group of eronegative women. Strong inverse correlations were revealed. Morphologically, there were signs of delayed formation of villi, decidualization of the endometrial stroma and the formation of uteroplacental vessels. Conclusion. In seropositive women with spontaneous abortion associated with reactivation of cytomegaloviral infection, a strong association of high HIF-1α values with decrease in α-tocopherol and docosahexaenoic acid in the peripheral blood was revealed, which can be considered as an unfavorable factor in the disruption of placenta formation.N. A. IshutinaI. A. AndrievskayaN. G. PrikhodkoScientific Сentre for Family Health and Human Reproduction Problemsarticlepregnancycytomegalovirus infectionhypoxia-inducible factor 1αα-tocopheroldocosahexaenoic acidScienceQRUActa Biomedica Scientifica, Vol 6, Iss 3, Pp 43-52 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	pregnancy cytomegalovirus infection hypoxia-inducible factor 1α α-tocopherol docosahexaenoic acid Science Q
spellingShingle	pregnancy cytomegalovirus infection hypoxia-inducible factor 1α α-tocopherol docosahexaenoic acid Science Q N. A. Ishutina I. A. Andrievskaya N. G. Prikhodko A new approach to assessment of reproductive losses of the first trimester of pregnancy
description	Background. At present, the role of cytomegalovirus infection in the disruption of acental formation and associated pathological conditions of the fetus is bvious, which are based on oxidative and inflammatory reactions that cause disruption of igration and invasion of trophoblasts, angiogenic modulation of placental and uterine vessels.Objective: To analyze the quantitative indicators of hypoxia-inducible factor 1α, α-tocopherol and docosahexaenoic acid in the peripheral blood in comparison with morphological changes in the placenta and uterine mucosa in seropositive women with spontaneous abortion associated with reactivation of ytomegaloviral infection.Methods: Studies were carried out at 7-9 weeks of gestation. 67 observations were analyzed, including 35 seropositive women in the first trimester with spontaneous abortion associated with reactivation of cytomegaloviral infection and 32 healthy seronerative women with voluntary termination of pregnancy. Stabilized blood from vein, epithelium of the inner surface of the cheek, cervical canal, urine, villous chorion, and uterine mucosa were used as the test material. Enzyme immunoassay was used to detect serological markers of cytomegaloviral infection (CMV-IgG/IgM antibodies, CMV-IgG avidity), hypoxia-induced factor 1α, α-tocopherol – by fluorometric method, PCR (polymerase chain reaction) in real time – DNA CMV, the method of gas-liquid chromatography – docosahexaenoic acid, histological methods – the general plan of the structure of the chorionic villus and the mucous membrane of the uterus.Results: In seropositive women with spontaneous abortion associated with reactivation of cytomegaloviral infection, the average of hypoxia-induced factor 1α was 1.54 times higher, α-tocopherol and docosahexaenoic acid were 1.71 times lower and 1.40 times lower (p < 0.001, respectively) than in the group of eronegative women. Strong inverse correlations were revealed. Morphologically, there were signs of delayed formation of villi, decidualization of the endometrial stroma and the formation of uteroplacental vessels. Conclusion. In seropositive women with spontaneous abortion associated with reactivation of cytomegaloviral infection, a strong association of high HIF-1α values with decrease in α-tocopherol and docosahexaenoic acid in the peripheral blood was revealed, which can be considered as an unfavorable factor in the disruption of placenta formation.
format	article
author	N. A. Ishutina I. A. Andrievskaya N. G. Prikhodko
author_facet	N. A. Ishutina I. A. Andrievskaya N. G. Prikhodko
author_sort	N. A. Ishutina
title	A new approach to assessment of reproductive losses of the first trimester of pregnancy
title_short	A new approach to assessment of reproductive losses of the first trimester of pregnancy
title_full	A new approach to assessment of reproductive losses of the first trimester of pregnancy
title_fullStr	A new approach to assessment of reproductive losses of the first trimester of pregnancy
title_full_unstemmed	A new approach to assessment of reproductive losses of the first trimester of pregnancy
title_sort	new approach to assessment of reproductive losses of the first trimester of pregnancy
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2021
url	https://doaj.org/article/77855a45eb3d4e92a06bffeec7e812dc
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A new approach to assessment of reproductive losses of the first trimester of pregnancy

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