Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

The ability to produce homogeneous glycoproteins is expected to advance fundamental understanding in glycoscience, but current in vivo-based production systems have several limitations. Here, the authors develop an E. coli extract-based one-pot system for customized production of N-linked glycoprote...

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Autores principales:	Thapakorn Jaroentomeechai, Jessica C. Stark, Aravind Natarajan, Cameron J. Glasscock, Laura E. Yates, Karen J. Hsu, Milan Mrksich, Michael C. Jewett, Matthew P. DeLisa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/7787cd01450a4f779c5957f555943da9
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spelling	oai:doaj.org-article:7787cd01450a4f779c5957f555943da92021-12-02T15:34:47ZSingle-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery10.1038/s41467-018-05110-x2041-1723https://doaj.org/article/7787cd01450a4f779c5957f555943da92018-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05110-xhttps://doaj.org/toc/2041-1723The ability to produce homogeneous glycoproteins is expected to advance fundamental understanding in glycoscience, but current in vivo-based production systems have several limitations. Here, the authors develop an E. coli extract-based one-pot system for customized production of N-linked glycoproteins.Thapakorn JaroentomeechaiJessica C. StarkAravind NatarajanCameron J. GlasscockLaura E. YatesKaren J. HsuMilan MrksichMichael C. JewettMatthew P. DeLisaNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Thapakorn Jaroentomeechai Jessica C. Stark Aravind Natarajan Cameron J. Glasscock Laura E. Yates Karen J. Hsu Milan Mrksich Michael C. Jewett Matthew P. DeLisa Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
description	The ability to produce homogeneous glycoproteins is expected to advance fundamental understanding in glycoscience, but current in vivo-based production systems have several limitations. Here, the authors develop an E. coli extract-based one-pot system for customized production of N-linked glycoproteins.
format	article
author	Thapakorn Jaroentomeechai Jessica C. Stark Aravind Natarajan Cameron J. Glasscock Laura E. Yates Karen J. Hsu Milan Mrksich Michael C. Jewett Matthew P. DeLisa
author_facet	Thapakorn Jaroentomeechai Jessica C. Stark Aravind Natarajan Cameron J. Glasscock Laura E. Yates Karen J. Hsu Milan Mrksich Michael C. Jewett Matthew P. DeLisa
author_sort	Thapakorn Jaroentomeechai
title	Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
title_short	Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
title_full	Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
title_fullStr	Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
title_full_unstemmed	Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
title_sort	single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/7787cd01450a4f779c5957f555943da9
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Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

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