Comparative proteomic analysis to annotate the structural and functional association of the hypothetical proteins of S. maltophilia k279a and predict potential T and B cell targets for vaccination.

Stenotrophomonas maltophilia is a multidrug-resistant bacterium with no precise clinical treatment. This bacterium can be a vital cause for death and different organ failures in immune-compromised, immune-competent, and long-time hospitalized patients. Extensive quorum sensing capability has become...

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Autores principales:	Md Muzahid Ahmed Ezaj, Md Sajedul Haque, Shifath Bin Syed, Md Shakil Ahmed Khan, Kazi Rejvee Ahmed, Mst Tania Khatun, S M Abdul Nayeem, Golam Rosul Rizvi, Mohammad Al-Forkan, Laila Khaleda
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/778befae48804bcc8d6a21e24a0c5fbe
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spelling	oai:doaj.org-article:778befae48804bcc8d6a21e24a0c5fbe2021-11-25T06:23:40ZComparative proteomic analysis to annotate the structural and functional association of the hypothetical proteins of S. maltophilia k279a and predict potential T and B cell targets for vaccination.1932-620310.1371/journal.pone.0252295https://doaj.org/article/778befae48804bcc8d6a21e24a0c5fbe2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252295https://doaj.org/toc/1932-6203Stenotrophomonas maltophilia is a multidrug-resistant bacterium with no precise clinical treatment. This bacterium can be a vital cause for death and different organ failures in immune-compromised, immune-competent, and long-time hospitalized patients. Extensive quorum sensing capability has become a challenge to develop new drugs against this pathogen. Moreover, the organism possesses about 789 proteins which function, structure, and pathogenesis remain obscured. In this piece of work, we tried to enlighten the aforementioned sectors using highly reliable bioinformatics tools validated by the scientific community. At first, the whole proteome sequence of the organism was retrieved and stored. Then we separated the hypothetical proteins and searched for the conserved domain with a high confidence level and multi-server validation, which resulted in 24 such proteins. Furthermore, all of their physical and chemical characterizations were performed, such as theoretical isoelectric point, molecular weight, GRAVY value, and many more. Besides, the subcellular localization, protein-protein interactions, functional motifs, 3D structures, antigenicity, and virulence factors were also evaluated. As an extension of this work, 'RTFAMSSER' and 'PAAPQPSAS' were predicted as potential T and B cell epitopes, respectively. We hope our findings will help in better understating the pathogenesis and smoothen the way to the cure.Md Muzahid Ahmed EzajMd Sajedul HaqueShifath Bin SyedMd Shakil Ahmed KhanKazi Rejvee AhmedMst Tania KhatunS M Abdul NayeemGolam Rosul RizviMohammad Al-ForkanLaila KhaledaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0252295 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Md Muzahid Ahmed Ezaj Md Sajedul Haque Shifath Bin Syed Md Shakil Ahmed Khan Kazi Rejvee Ahmed Mst Tania Khatun S M Abdul Nayeem Golam Rosul Rizvi Mohammad Al-Forkan Laila Khaleda Comparative proteomic analysis to annotate the structural and functional association of the hypothetical proteins of S. maltophilia k279a and predict potential T and B cell targets for vaccination.
description	Stenotrophomonas maltophilia is a multidrug-resistant bacterium with no precise clinical treatment. This bacterium can be a vital cause for death and different organ failures in immune-compromised, immune-competent, and long-time hospitalized patients. Extensive quorum sensing capability has become a challenge to develop new drugs against this pathogen. Moreover, the organism possesses about 789 proteins which function, structure, and pathogenesis remain obscured. In this piece of work, we tried to enlighten the aforementioned sectors using highly reliable bioinformatics tools validated by the scientific community. At first, the whole proteome sequence of the organism was retrieved and stored. Then we separated the hypothetical proteins and searched for the conserved domain with a high confidence level and multi-server validation, which resulted in 24 such proteins. Furthermore, all of their physical and chemical characterizations were performed, such as theoretical isoelectric point, molecular weight, GRAVY value, and many more. Besides, the subcellular localization, protein-protein interactions, functional motifs, 3D structures, antigenicity, and virulence factors were also evaluated. As an extension of this work, 'RTFAMSSER' and 'PAAPQPSAS' were predicted as potential T and B cell epitopes, respectively. We hope our findings will help in better understating the pathogenesis and smoothen the way to the cure.
format	article
author	Md Muzahid Ahmed Ezaj Md Sajedul Haque Shifath Bin Syed Md Shakil Ahmed Khan Kazi Rejvee Ahmed Mst Tania Khatun S M Abdul Nayeem Golam Rosul Rizvi Mohammad Al-Forkan Laila Khaleda
author_facet	Md Muzahid Ahmed Ezaj Md Sajedul Haque Shifath Bin Syed Md Shakil Ahmed Khan Kazi Rejvee Ahmed Mst Tania Khatun S M Abdul Nayeem Golam Rosul Rizvi Mohammad Al-Forkan Laila Khaleda
author_sort	Md Muzahid Ahmed Ezaj
title	Comparative proteomic analysis to annotate the structural and functional association of the hypothetical proteins of S. maltophilia k279a and predict potential T and B cell targets for vaccination.
title_short	Comparative proteomic analysis to annotate the structural and functional association of the hypothetical proteins of S. maltophilia k279a and predict potential T and B cell targets for vaccination.
title_full	Comparative proteomic analysis to annotate the structural and functional association of the hypothetical proteins of S. maltophilia k279a and predict potential T and B cell targets for vaccination.
title_fullStr	Comparative proteomic analysis to annotate the structural and functional association of the hypothetical proteins of S. maltophilia k279a and predict potential T and B cell targets for vaccination.
title_full_unstemmed	Comparative proteomic analysis to annotate the structural and functional association of the hypothetical proteins of S. maltophilia k279a and predict potential T and B cell targets for vaccination.
title_sort	comparative proteomic analysis to annotate the structural and functional association of the hypothetical proteins of s. maltophilia k279a and predict potential t and b cell targets for vaccination.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/778befae48804bcc8d6a21e24a0c5fbe
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Comparative proteomic analysis to annotate the structural and functional association of the hypothetical proteins of S. maltophilia k279a and predict potential T and B cell targets for vaccination.

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