Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy.

Tendon injuries are a common age-related degenerative condition where current treatment strategies fail to restore functionality and normal quality of life. This disease also occurs naturally in horses, with many similarities to human tendinopathy making it an ideal large animal model for human dise...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Roger Kenneth Whealands Smith, Natalie Jayne Werling, Stephanie Georgina Dakin, Rafiqul Alam, Allen E Goodship, Jayesh Dudhia
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/778c6b129b684afe9d1b2ec2f28c5d93
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:778c6b129b684afe9d1b2ec2f28c5d93
record_format	dspace
spelling	oai:doaj.org-article:778c6b129b684afe9d1b2ec2f28c5d932021-11-18T08:53:51ZBeneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy.1932-620310.1371/journal.pone.0075697https://doaj.org/article/778c6b129b684afe9d1b2ec2f28c5d932013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24086616/?tool=EBIhttps://doaj.org/toc/1932-6203Tendon injuries are a common age-related degenerative condition where current treatment strategies fail to restore functionality and normal quality of life. This disease also occurs naturally in horses, with many similarities to human tendinopathy making it an ideal large animal model for human disease. Regenerative approaches are increasingly used to improve outcome involving mesenchymal stem cells (MSCs), supported by clinical data where injection of autologous bone marrow derived MSCs (BM-MSCs) suspended in marrow supernatant into injured tendons has halved the re-injury rate in racehorses. We hypothesized that stem cell therapy induces a matrix more closely resembling normal tendon than the fibrous scar tissue formed by natural repair. Twelve horses with career-ending naturally-occurring superficial digital flexor tendon injury were allocated randomly to treatment and control groups. 1X10(7) autologous BM-MSCs suspended in 2 ml of marrow supernatant were implanted into the damaged tendon of the treated group. The control group received the same volume of saline. Following a 6 month exercise programme horses were euthanized and tendons assessed for structural stiffness by non-destructive mechanical testing and for morphological and molecular composition. BM-MSC treated tendons exhibited statistically significant improvements in key parameters compared to saline-injected control tendons towards that of normal tendons and those in the contralateral limbs. Specifically, treated tendons had lower structural stiffness (p<0.05) although no significant difference in calculated modulus of elasticity, lower (improved) histological scoring of organisation (p<0.003) and crimp pattern (p<0.05), lower cellularity (p<0.007), DNA content (p<0.05), vascularity (p<0.03), water content (p<0.05), GAG content (p<0.05), and MMP-13 activity (p<0.02). Treatment with autologous MSCs in marrow supernatant therefore provides significant benefits compared to untreated tendon repair in enhancing normalisation of biomechanical, morphological, and compositional parameters. These data in natural disease, with no adverse findings, support the use of this treatment for human tendon injuries.Roger Kenneth Whealands SmithNatalie Jayne WerlingStephanie Georgina DakinRafiqul AlamAllen E GoodshipJayesh DudhiaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e75697 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Roger Kenneth Whealands Smith Natalie Jayne Werling Stephanie Georgina Dakin Rafiqul Alam Allen E Goodship Jayesh Dudhia Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy.
description	Tendon injuries are a common age-related degenerative condition where current treatment strategies fail to restore functionality and normal quality of life. This disease also occurs naturally in horses, with many similarities to human tendinopathy making it an ideal large animal model for human disease. Regenerative approaches are increasingly used to improve outcome involving mesenchymal stem cells (MSCs), supported by clinical data where injection of autologous bone marrow derived MSCs (BM-MSCs) suspended in marrow supernatant into injured tendons has halved the re-injury rate in racehorses. We hypothesized that stem cell therapy induces a matrix more closely resembling normal tendon than the fibrous scar tissue formed by natural repair. Twelve horses with career-ending naturally-occurring superficial digital flexor tendon injury were allocated randomly to treatment and control groups. 1X10(7) autologous BM-MSCs suspended in 2 ml of marrow supernatant were implanted into the damaged tendon of the treated group. The control group received the same volume of saline. Following a 6 month exercise programme horses were euthanized and tendons assessed for structural stiffness by non-destructive mechanical testing and for morphological and molecular composition. BM-MSC treated tendons exhibited statistically significant improvements in key parameters compared to saline-injected control tendons towards that of normal tendons and those in the contralateral limbs. Specifically, treated tendons had lower structural stiffness (p<0.05) although no significant difference in calculated modulus of elasticity, lower (improved) histological scoring of organisation (p<0.003) and crimp pattern (p<0.05), lower cellularity (p<0.007), DNA content (p<0.05), vascularity (p<0.03), water content (p<0.05), GAG content (p<0.05), and MMP-13 activity (p<0.02). Treatment with autologous MSCs in marrow supernatant therefore provides significant benefits compared to untreated tendon repair in enhancing normalisation of biomechanical, morphological, and compositional parameters. These data in natural disease, with no adverse findings, support the use of this treatment for human tendon injuries.
format	article
author	Roger Kenneth Whealands Smith Natalie Jayne Werling Stephanie Georgina Dakin Rafiqul Alam Allen E Goodship Jayesh Dudhia
author_facet	Roger Kenneth Whealands Smith Natalie Jayne Werling Stephanie Georgina Dakin Rafiqul Alam Allen E Goodship Jayesh Dudhia
author_sort	Roger Kenneth Whealands Smith
title	Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy.
title_short	Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy.
title_full	Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy.
title_fullStr	Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy.
title_full_unstemmed	Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy.
title_sort	beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/778c6b129b684afe9d1b2ec2f28c5d93
work_keys_str_mv	AT rogerkennethwhealandssmith beneficialeffectsofautologousbonemarrowderivedmesenchymalstemcellsinnaturallyoccurringtendinopathy AT nataliejaynewerling beneficialeffectsofautologousbonemarrowderivedmesenchymalstemcellsinnaturallyoccurringtendinopathy AT stephaniegeorginadakin beneficialeffectsofautologousbonemarrowderivedmesenchymalstemcellsinnaturallyoccurringtendinopathy AT rafiqulalam beneficialeffectsofautologousbonemarrowderivedmesenchymalstemcellsinnaturallyoccurringtendinopathy AT allenegoodship beneficialeffectsofautologousbonemarrowderivedmesenchymalstemcellsinnaturallyoccurringtendinopathy AT jayeshdudhia beneficialeffectsofautologousbonemarrowderivedmesenchymalstemcellsinnaturallyoccurringtendinopathy
_version_	1718421209762234368

Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy.

Ejemplares similares