Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study

Georgia Kastrinaki,1,* Christos Samsouris,2,* Efstratios K Kosmidis,3 Eleni Papaioannou,1 Athanasios G Konstandopoulos,1,4 George Theophilidis2 1Aerosol and Particle Technology Laboratory (APTL), CERTH/CPERI, Thessaloniki, Greece; 2Laboratory of Animal Physiology, School of Biology, Aristotle Univer...

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Autores principales:	Kastrinaki G, Samsouris C, Kosmidis EK, Papaioannou E, Konstandopoulos AG, Theophilidis G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7790228aaca4407096d635462b25ba18
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spelling	oai:doaj.org-article:7790228aaca4407096d635462b25ba182021-12-02T06:47:14ZAssessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study1178-2013https://doaj.org/article/7790228aaca4407096d635462b25ba182015-11-01T00:00:00Zhttps://www.dovepress.com/assessing-the-axonal-translocation-of-ceo2-and-sio2-nanoparticles-in-t-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Georgia Kastrinaki,1,* Christos Samsouris,2,* Efstratios K Kosmidis,3 Eleni Papaioannou,1 Athanasios G Konstandopoulos,1,4 George Theophilidis2 1Aerosol and Particle Technology Laboratory (APTL), CERTH/CPERI, Thessaloniki, Greece; 2Laboratory of Animal Physiology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki, Greece; 3Laboratory of Physiology, Department of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece; 4Department of Chemical Engineering, Aristotle University of Thessaloniki, Thessaloniki, Greece *These authors contributed equally to this work Abstract: The axonal translocation of two commonly used nanoparticles in medicine, namely CeO2 and SiO2, is investigated. The study was conducted on frog sciatic nerve fibers in an ex vivo preparation. Nanoparticles were applied at the proximal end of the excised nerve. A nerve stimulation protocol was followed for over 35 hours. Nerve vitality curve comparison between control and exposed nerves showed that CeO2 has no neurotoxic effect at the concentrations tested. After exposure, specimens were fixed and then screen scanned every 1 mm along their length for nanoparticle presence by means of Fourier transform infrared microscopy. We demonstrated that both nanoparticles translocate within the nerve by formation of narrow bands in the Fourier transform infrared spectrum. For the CeO2, we also demonstrated that the translocation depends on both axonal integrity and electrical activity. The speed of translocation for the two species was estimated in the range of 0.45–0.58 mm/h, close to slow axonal transportation rate. Transmission electron microscopy provided direct evidence for the presence of SiO2 in the treated nerves. Keywords: CeO2, SiO2, FTIR, nanoparticles, ex vivo electrophysiology, frog sciatic nerve, translocationKastrinaki GSamsouris CKosmidis EKPapaioannou EKonstandopoulos AGTheophilidis GDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 7089-7096 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kastrinaki G Samsouris C Kosmidis EK Papaioannou E Konstandopoulos AG Theophilidis G Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study
description	Georgia Kastrinaki,1,* Christos Samsouris,2,* Efstratios K Kosmidis,3 Eleni Papaioannou,1 Athanasios G Konstandopoulos,1,4 George Theophilidis2 1Aerosol and Particle Technology Laboratory (APTL), CERTH/CPERI, Thessaloniki, Greece; 2Laboratory of Animal Physiology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki, Greece; 3Laboratory of Physiology, Department of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece; 4Department of Chemical Engineering, Aristotle University of Thessaloniki, Thessaloniki, Greece *These authors contributed equally to this work Abstract: The axonal translocation of two commonly used nanoparticles in medicine, namely CeO2 and SiO2, is investigated. The study was conducted on frog sciatic nerve fibers in an ex vivo preparation. Nanoparticles were applied at the proximal end of the excised nerve. A nerve stimulation protocol was followed for over 35 hours. Nerve vitality curve comparison between control and exposed nerves showed that CeO2 has no neurotoxic effect at the concentrations tested. After exposure, specimens were fixed and then screen scanned every 1 mm along their length for nanoparticle presence by means of Fourier transform infrared microscopy. We demonstrated that both nanoparticles translocate within the nerve by formation of narrow bands in the Fourier transform infrared spectrum. For the CeO2, we also demonstrated that the translocation depends on both axonal integrity and electrical activity. The speed of translocation for the two species was estimated in the range of 0.45–0.58 mm/h, close to slow axonal transportation rate. Transmission electron microscopy provided direct evidence for the presence of SiO2 in the treated nerves. Keywords: CeO2, SiO2, FTIR, nanoparticles, ex vivo electrophysiology, frog sciatic nerve, translocation
format	article
author	Kastrinaki G Samsouris C Kosmidis EK Papaioannou E Konstandopoulos AG Theophilidis G
author_facet	Kastrinaki G Samsouris C Kosmidis EK Papaioannou E Konstandopoulos AG Theophilidis G
author_sort	Kastrinaki G
title	Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study
title_short	Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study
title_full	Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study
title_fullStr	Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study
title_full_unstemmed	Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study
title_sort	assessing the axonal translocation of ceo2 and sio2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/7790228aaca4407096d635462b25ba18
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Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study

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