Dynamic Imaging of CD8(+) T cells and dendritic cells during infection with Toxoplasma gondii.

To better understand the initiation of CD8(+) T cell responses during infection, the primary response to the intracellular parasite Toxoplasma gondii was characterized using 2-photon microscopy combined with an experimental system that allowed visualization of dendritic cells (DCs) and parasite spec...

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Autores principales:	Beena John, Tajie H Harris, Elia D Tait, Emma H Wilson, Beth Gregg, Lai Guan Ng, Paulus Mrass, David S Roos, Florence Dzierszinski, Wolfgang Weninger, Christopher A Hunter
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/779855c1113d44ffa2f2cba1b1e360d0
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spelling	oai:doaj.org-article:779855c1113d44ffa2f2cba1b1e360d02021-11-25T05:47:47ZDynamic Imaging of CD8(+) T cells and dendritic cells during infection with Toxoplasma gondii.1553-73661553-737410.1371/journal.ppat.1000505https://doaj.org/article/779855c1113d44ffa2f2cba1b1e360d02009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19578440/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374To better understand the initiation of CD8(+) T cell responses during infection, the primary response to the intracellular parasite Toxoplasma gondii was characterized using 2-photon microscopy combined with an experimental system that allowed visualization of dendritic cells (DCs) and parasite specific CD8(+) T cells. Infection with T. gondii induced localization of both these populations to the sub-capsular/interfollicular region of the draining lymph node and DCs were required for the expansion of the T cells. Consistent with current models, in the presence of cognate antigen, the average velocity of CD8(+) T cells decreased. Unexpectedly, infection also resulted in modulation of the behavior of non-parasite specific T cells. This TCR-independent process correlated with the re-modeling of the lymph node micro-architecture and changes in expression of CCL21 and CCL3. Infection also resulted in sustained interactions between the DCs and CD8(+) T cells that were visualized only in the presence of cognate antigen and were limited to an early phase in the response. Infected DCs were rare within the lymph node during this time frame; however, DCs presenting the cognate antigen were detected. Together, these data provide novel insights into the earliest interaction between DCs and CD8(+) T cells and suggest that cross presentation by bystander DCs rather than infected DCs is an important route of antigen presentation during toxoplasmosis.Beena JohnTajie H HarrisElia D TaitEmma H WilsonBeth GreggLai Guan NgPaulus MrassDavid S RoosFlorence DzierszinskiWolfgang WeningerChristopher A HunterPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 7, p e1000505 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Beena John Tajie H Harris Elia D Tait Emma H Wilson Beth Gregg Lai Guan Ng Paulus Mrass David S Roos Florence Dzierszinski Wolfgang Weninger Christopher A Hunter Dynamic Imaging of CD8(+) T cells and dendritic cells during infection with Toxoplasma gondii.
description	To better understand the initiation of CD8(+) T cell responses during infection, the primary response to the intracellular parasite Toxoplasma gondii was characterized using 2-photon microscopy combined with an experimental system that allowed visualization of dendritic cells (DCs) and parasite specific CD8(+) T cells. Infection with T. gondii induced localization of both these populations to the sub-capsular/interfollicular region of the draining lymph node and DCs were required for the expansion of the T cells. Consistent with current models, in the presence of cognate antigen, the average velocity of CD8(+) T cells decreased. Unexpectedly, infection also resulted in modulation of the behavior of non-parasite specific T cells. This TCR-independent process correlated with the re-modeling of the lymph node micro-architecture and changes in expression of CCL21 and CCL3. Infection also resulted in sustained interactions between the DCs and CD8(+) T cells that were visualized only in the presence of cognate antigen and were limited to an early phase in the response. Infected DCs were rare within the lymph node during this time frame; however, DCs presenting the cognate antigen were detected. Together, these data provide novel insights into the earliest interaction between DCs and CD8(+) T cells and suggest that cross presentation by bystander DCs rather than infected DCs is an important route of antigen presentation during toxoplasmosis.
format	article
author	Beena John Tajie H Harris Elia D Tait Emma H Wilson Beth Gregg Lai Guan Ng Paulus Mrass David S Roos Florence Dzierszinski Wolfgang Weninger Christopher A Hunter
author_facet	Beena John Tajie H Harris Elia D Tait Emma H Wilson Beth Gregg Lai Guan Ng Paulus Mrass David S Roos Florence Dzierszinski Wolfgang Weninger Christopher A Hunter
author_sort	Beena John
title	Dynamic Imaging of CD8(+) T cells and dendritic cells during infection with Toxoplasma gondii.
title_short	Dynamic Imaging of CD8(+) T cells and dendritic cells during infection with Toxoplasma gondii.
title_full	Dynamic Imaging of CD8(+) T cells and dendritic cells during infection with Toxoplasma gondii.
title_fullStr	Dynamic Imaging of CD8(+) T cells and dendritic cells during infection with Toxoplasma gondii.
title_full_unstemmed	Dynamic Imaging of CD8(+) T cells and dendritic cells during infection with Toxoplasma gondii.
title_sort	dynamic imaging of cd8(+) t cells and dendritic cells during infection with toxoplasma gondii.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/779855c1113d44ffa2f2cba1b1e360d0
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Dynamic Imaging of CD8(+) T cells and dendritic cells during infection with Toxoplasma gondii.

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