The antagonistic Metschnikowia andauensis produces extracellular enzymes and pulcherrimin, whose production can be promoted by the culture factors
Abstract Biological control against microbial infections has a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution. The pigment producer Metschnikowia andauensis belongs to the antagonistic yeasts, but details of the mechanism by which...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/779c76e4fb0b4754ad4451eea66d4e19 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:779c76e4fb0b4754ad4451eea66d4e19 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:779c76e4fb0b4754ad4451eea66d4e192021-12-02T14:58:37ZThe antagonistic Metschnikowia andauensis produces extracellular enzymes and pulcherrimin, whose production can be promoted by the culture factors10.1038/s41598-021-89982-y2045-2322https://doaj.org/article/779c76e4fb0b4754ad4451eea66d4e192021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89982-yhttps://doaj.org/toc/2045-2322Abstract Biological control against microbial infections has a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution. The pigment producer Metschnikowia andauensis belongs to the antagonistic yeasts, but details of the mechanism by which it inhibits growth of other microbes are less known. Our results confirmed its antagonistic capacity on other yeast species isolated from fruits or flowers and demonstrated that the antagonistic capacity was well correlated with the size of the red pigmented zone. We have isolated and characterized its red pigment, which proved to be the iron chelating pulcherrimin. Its production was possible even in the presence of 0.05 mg/ml copper sulphate, which is widely used in organic vineyards because of its antimicrobial properties. Production and localisation of the pulcherrimin strongly depended on composition of the media and other culture factors. Glucose, galactose, disaccharides and the presence of pectin or certain amino acids clearly promoted pigment production. Higher temperatures and iron concentration decreased the diameter of red pigmented zones. The effect of pH on pigment production varied depending of whether it was tested in liquid or solid media. In addition, our results suggest that other mechanisms besides the iron depletion of the culture media may contribute to the antagonistic capacity of M. andauensis.Enikő HorváthLívia DályaiErna SzabóTeréz BarnaLászló KalmárJózsef PostaMatthias SipiczkiHajnalka CsomaIda MiklósNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Enikő Horváth Lívia Dályai Erna Szabó Teréz Barna László Kalmár József Posta Matthias Sipiczki Hajnalka Csoma Ida Miklós The antagonistic Metschnikowia andauensis produces extracellular enzymes and pulcherrimin, whose production can be promoted by the culture factors |
| description |
Abstract Biological control against microbial infections has a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution. The pigment producer Metschnikowia andauensis belongs to the antagonistic yeasts, but details of the mechanism by which it inhibits growth of other microbes are less known. Our results confirmed its antagonistic capacity on other yeast species isolated from fruits or flowers and demonstrated that the antagonistic capacity was well correlated with the size of the red pigmented zone. We have isolated and characterized its red pigment, which proved to be the iron chelating pulcherrimin. Its production was possible even in the presence of 0.05 mg/ml copper sulphate, which is widely used in organic vineyards because of its antimicrobial properties. Production and localisation of the pulcherrimin strongly depended on composition of the media and other culture factors. Glucose, galactose, disaccharides and the presence of pectin or certain amino acids clearly promoted pigment production. Higher temperatures and iron concentration decreased the diameter of red pigmented zones. The effect of pH on pigment production varied depending of whether it was tested in liquid or solid media. In addition, our results suggest that other mechanisms besides the iron depletion of the culture media may contribute to the antagonistic capacity of M. andauensis. |
| format |
article |
| author |
Enikő Horváth Lívia Dályai Erna Szabó Teréz Barna László Kalmár József Posta Matthias Sipiczki Hajnalka Csoma Ida Miklós |
| author_facet |
Enikő Horváth Lívia Dályai Erna Szabó Teréz Barna László Kalmár József Posta Matthias Sipiczki Hajnalka Csoma Ida Miklós |
| author_sort |
Enikő Horváth |
| title |
The antagonistic Metschnikowia andauensis produces extracellular enzymes and pulcherrimin, whose production can be promoted by the culture factors |
| title_short |
The antagonistic Metschnikowia andauensis produces extracellular enzymes and pulcherrimin, whose production can be promoted by the culture factors |
| title_full |
The antagonistic Metschnikowia andauensis produces extracellular enzymes and pulcherrimin, whose production can be promoted by the culture factors |
| title_fullStr |
The antagonistic Metschnikowia andauensis produces extracellular enzymes and pulcherrimin, whose production can be promoted by the culture factors |
| title_full_unstemmed |
The antagonistic Metschnikowia andauensis produces extracellular enzymes and pulcherrimin, whose production can be promoted by the culture factors |
| title_sort |
antagonistic metschnikowia andauensis produces extracellular enzymes and pulcherrimin, whose production can be promoted by the culture factors |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/779c76e4fb0b4754ad4451eea66d4e19 |
| work_keys_str_mv |
AT enikohorvath theantagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT liviadalyai theantagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT ernaszabo theantagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT terezbarna theantagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT laszlokalmar theantagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT jozsefposta theantagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT matthiassipiczki theantagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT hajnalkacsoma theantagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT idamiklos theantagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT enikohorvath antagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT liviadalyai antagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT ernaszabo antagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT terezbarna antagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT laszlokalmar antagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT jozsefposta antagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT matthiassipiczki antagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT hajnalkacsoma antagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors AT idamiklos antagonisticmetschnikowiaandauensisproducesextracellularenzymesandpulcherriminwhoseproductioncanbepromotedbytheculturefactors |
| _version_ |
1718389273362694144 |