Two odorant-binding proteins mediate the behavioural response of aphids to the alarm pheromone (E)-ß-farnesene and structural analogues.

<h4>Background</h4>Aphids are agricultural pests of great economical interest. Alternatives to insecticides, using semiochemicals, are of difficult applications. In fact, sex pheromones are of little use as aphids reproduce partenogenetically most of the time. Besides, the alarm pheromon...

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Autores principales:	Yu Feng Sun, Filomena De Biasio, Hui Li Qiao, Immacolata Iovinella, Shao Xiang Yang, Yun Ling, Lea Riviello, Donatella Battaglia, Patrizia Falabella, Xin Ling Yang, Paolo Pelosi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/779dad60a9af475687b0b3c046562a6b
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spelling	oai:doaj.org-article:779dad60a9af475687b0b3c046562a6b2021-11-18T07:25:32ZTwo odorant-binding proteins mediate the behavioural response of aphids to the alarm pheromone (E)-ß-farnesene and structural analogues.1932-620310.1371/journal.pone.0032759https://doaj.org/article/779dad60a9af475687b0b3c046562a6b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22427877/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Aphids are agricultural pests of great economical interest. Alternatives to insecticides, using semiochemicals, are of difficult applications. In fact, sex pheromones are of little use as aphids reproduce partenogenetically most of the time. Besides, the alarm pheromone, (E)-ß-farnesene for a great number of species, is difficult to synthesize and unstable in the environment. The search for novel semiochemicals to be used in population control can be efficiently approached through the study of the olfactory system at the biochemical level. Recently odorant-binding proteins (OBPs) have been shown to play a central role in olfactory recognition, thus becoming the target of choice for designing new semiochemicals.<h4>Methodology/principal findings</h4>To address the question of how the alarm message is recognised at the level of OBPs, we have tested 29 compounds, including (E)-ß-farnesene, in binding assays with 6 recombinant proteins and in behaviour experiments. We have found that good repellents bind OBP3 and/or OBP7, while non repellents present different spectra of binding. These results have been verified with two species of aphids, Acyrthosiphon pisum and Myzus persicae, both using (E)-ß-farnesene as the alarm pheromone.<h4>Conclusions</h4>Our results represent further support to the idea (so far convincingly demonstrated only in Drosophila) that OBPs are involved in decoding the chemical information of odorants and pheromones, and for the first time provide such evidence in other insect species and using wild-type insects. Moreover, the data offer guidelines and protocols for the discovery of potential alarm pheromones, using ligand-binding assays as a preliminary screening before subjecting selected compounds to behaviour tests.Yu Feng SunFilomena De BiasioHui Li QiaoImmacolata IovinellaShao Xiang YangYun LingLea RivielloDonatella BattagliaPatrizia FalabellaXin Ling YangPaolo PelosiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e32759 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yu Feng Sun Filomena De Biasio Hui Li Qiao Immacolata Iovinella Shao Xiang Yang Yun Ling Lea Riviello Donatella Battaglia Patrizia Falabella Xin Ling Yang Paolo Pelosi Two odorant-binding proteins mediate the behavioural response of aphids to the alarm pheromone (E)-ß-farnesene and structural analogues.
description	<h4>Background</h4>Aphids are agricultural pests of great economical interest. Alternatives to insecticides, using semiochemicals, are of difficult applications. In fact, sex pheromones are of little use as aphids reproduce partenogenetically most of the time. Besides, the alarm pheromone, (E)-ß-farnesene for a great number of species, is difficult to synthesize and unstable in the environment. The search for novel semiochemicals to be used in population control can be efficiently approached through the study of the olfactory system at the biochemical level. Recently odorant-binding proteins (OBPs) have been shown to play a central role in olfactory recognition, thus becoming the target of choice for designing new semiochemicals.<h4>Methodology/principal findings</h4>To address the question of how the alarm message is recognised at the level of OBPs, we have tested 29 compounds, including (E)-ß-farnesene, in binding assays with 6 recombinant proteins and in behaviour experiments. We have found that good repellents bind OBP3 and/or OBP7, while non repellents present different spectra of binding. These results have been verified with two species of aphids, Acyrthosiphon pisum and Myzus persicae, both using (E)-ß-farnesene as the alarm pheromone.<h4>Conclusions</h4>Our results represent further support to the idea (so far convincingly demonstrated only in Drosophila) that OBPs are involved in decoding the chemical information of odorants and pheromones, and for the first time provide such evidence in other insect species and using wild-type insects. Moreover, the data offer guidelines and protocols for the discovery of potential alarm pheromones, using ligand-binding assays as a preliminary screening before subjecting selected compounds to behaviour tests.
format	article
author	Yu Feng Sun Filomena De Biasio Hui Li Qiao Immacolata Iovinella Shao Xiang Yang Yun Ling Lea Riviello Donatella Battaglia Patrizia Falabella Xin Ling Yang Paolo Pelosi
author_facet	Yu Feng Sun Filomena De Biasio Hui Li Qiao Immacolata Iovinella Shao Xiang Yang Yun Ling Lea Riviello Donatella Battaglia Patrizia Falabella Xin Ling Yang Paolo Pelosi
author_sort	Yu Feng Sun
title	Two odorant-binding proteins mediate the behavioural response of aphids to the alarm pheromone (E)-ß-farnesene and structural analogues.
title_short	Two odorant-binding proteins mediate the behavioural response of aphids to the alarm pheromone (E)-ß-farnesene and structural analogues.
title_full	Two odorant-binding proteins mediate the behavioural response of aphids to the alarm pheromone (E)-ß-farnesene and structural analogues.
title_fullStr	Two odorant-binding proteins mediate the behavioural response of aphids to the alarm pheromone (E)-ß-farnesene and structural analogues.
title_full_unstemmed	Two odorant-binding proteins mediate the behavioural response of aphids to the alarm pheromone (E)-ß-farnesene and structural analogues.
title_sort	two odorant-binding proteins mediate the behavioural response of aphids to the alarm pheromone (e)-ß-farnesene and structural analogues.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/779dad60a9af475687b0b3c046562a6b
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Two odorant-binding proteins mediate the behavioural response of aphids to the alarm pheromone (E)-ß-farnesene and structural analogues.

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