Saccharomyces boulardii modifies Salmonella typhimurium traffic and host immune responses along the intestinal tract.
Salmonella enterica serovar Typhimurium (ST) is an enteropathogenic Gram-negative bacterium that causes infection following oral ingestion. ST spreads rapidly along the gastrointestinal tract (GIT) and invades the intestinal epithelium to ultimately reach internal body organs. The probiotic yeast Sa...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2014
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/77a6f04f6cc042308311a564395bc4b0 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:77a6f04f6cc042308311a564395bc4b0 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:77a6f04f6cc042308311a564395bc4b02021-11-25T06:05:01ZSaccharomyces boulardii modifies Salmonella typhimurium traffic and host immune responses along the intestinal tract.1932-620310.1371/journal.pone.0103069https://doaj.org/article/77a6f04f6cc042308311a564395bc4b02014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25118595/?tool=EBIhttps://doaj.org/toc/1932-6203Salmonella enterica serovar Typhimurium (ST) is an enteropathogenic Gram-negative bacterium that causes infection following oral ingestion. ST spreads rapidly along the gastrointestinal tract (GIT) and invades the intestinal epithelium to ultimately reach internal body organs. The probiotic yeast Saccharomyces boulardii BIOCODEX (S.b-B) is prescribed for prophylaxis of diarrheal infectious diseases. We previously showed that S.b-B prevents weight loss in ST-infected mice and significantly decreases bacterial translocation to the spleen and liver. This study was designed to investigate the effect of S.b-B on ST migration along the GIT and the impact of the yeast on the host's early innate immune responses. Bioluminescent imaging (BLI) was used to evaluate the effect of S.b-B on the progression of luminescent Salmonella Typhimurium (ST-lux) in the GIT of mice pretreated with streptomycin. Photonic emission (PE) was measured in GIT extracts (stomach, small intestine, cecum and colon) at various time periods post-infection (PI). PE analysis revealed that, 45 min PI, ST-lux had migrated slightly faster in the mice treated with S.b-B than in the untreated infected animals. At 90 min PI, ST-lux had reached the cecum in both groups of mice. Adhesion of ST to S.b-B was visualized in the intestines of the mice and probably accounts for (1) the faster elimination of ST-lux in the feces, and (2) reduced translocation of ST to the spleen and liver. In the early phase of infection, S.b-B also modifies the host's immune responses by (1) increasing IFN-γ gene expression and decreasing IL-10 gene expression in the small intestine, and (2) elevating both IFN-γ, and IL-10 mRNA levels in the cecum. BLI revealed that S.b-B modifies ST migration and the host immune response along the GIT. Study findings shed new light on the protective mechanisms of S.b-B during the early phase of Salmonella pathogenesis.Rodolphe Pontier-BresPatrick MunroLaurent BoyerRodolphe AntyVéronique ImbertChloé TercioloFréderic AndréPatrick RampalEmmanuel LemichezJean-François PeyronDorota CzeruckaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e103069 (2014) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Rodolphe Pontier-Bres Patrick Munro Laurent Boyer Rodolphe Anty Véronique Imbert Chloé Terciolo Fréderic André Patrick Rampal Emmanuel Lemichez Jean-François Peyron Dorota Czerucka Saccharomyces boulardii modifies Salmonella typhimurium traffic and host immune responses along the intestinal tract. |
| description |
Salmonella enterica serovar Typhimurium (ST) is an enteropathogenic Gram-negative bacterium that causes infection following oral ingestion. ST spreads rapidly along the gastrointestinal tract (GIT) and invades the intestinal epithelium to ultimately reach internal body organs. The probiotic yeast Saccharomyces boulardii BIOCODEX (S.b-B) is prescribed for prophylaxis of diarrheal infectious diseases. We previously showed that S.b-B prevents weight loss in ST-infected mice and significantly decreases bacterial translocation to the spleen and liver. This study was designed to investigate the effect of S.b-B on ST migration along the GIT and the impact of the yeast on the host's early innate immune responses. Bioluminescent imaging (BLI) was used to evaluate the effect of S.b-B on the progression of luminescent Salmonella Typhimurium (ST-lux) in the GIT of mice pretreated with streptomycin. Photonic emission (PE) was measured in GIT extracts (stomach, small intestine, cecum and colon) at various time periods post-infection (PI). PE analysis revealed that, 45 min PI, ST-lux had migrated slightly faster in the mice treated with S.b-B than in the untreated infected animals. At 90 min PI, ST-lux had reached the cecum in both groups of mice. Adhesion of ST to S.b-B was visualized in the intestines of the mice and probably accounts for (1) the faster elimination of ST-lux in the feces, and (2) reduced translocation of ST to the spleen and liver. In the early phase of infection, S.b-B also modifies the host's immune responses by (1) increasing IFN-γ gene expression and decreasing IL-10 gene expression in the small intestine, and (2) elevating both IFN-γ, and IL-10 mRNA levels in the cecum. BLI revealed that S.b-B modifies ST migration and the host immune response along the GIT. Study findings shed new light on the protective mechanisms of S.b-B during the early phase of Salmonella pathogenesis. |
| format |
article |
| author |
Rodolphe Pontier-Bres Patrick Munro Laurent Boyer Rodolphe Anty Véronique Imbert Chloé Terciolo Fréderic André Patrick Rampal Emmanuel Lemichez Jean-François Peyron Dorota Czerucka |
| author_facet |
Rodolphe Pontier-Bres Patrick Munro Laurent Boyer Rodolphe Anty Véronique Imbert Chloé Terciolo Fréderic André Patrick Rampal Emmanuel Lemichez Jean-François Peyron Dorota Czerucka |
| author_sort |
Rodolphe Pontier-Bres |
| title |
Saccharomyces boulardii modifies Salmonella typhimurium traffic and host immune responses along the intestinal tract. |
| title_short |
Saccharomyces boulardii modifies Salmonella typhimurium traffic and host immune responses along the intestinal tract. |
| title_full |
Saccharomyces boulardii modifies Salmonella typhimurium traffic and host immune responses along the intestinal tract. |
| title_fullStr |
Saccharomyces boulardii modifies Salmonella typhimurium traffic and host immune responses along the intestinal tract. |
| title_full_unstemmed |
Saccharomyces boulardii modifies Salmonella typhimurium traffic and host immune responses along the intestinal tract. |
| title_sort |
saccharomyces boulardii modifies salmonella typhimurium traffic and host immune responses along the intestinal tract. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/77a6f04f6cc042308311a564395bc4b0 |
| work_keys_str_mv |
AT rodolphepontierbres saccharomycesboulardiimodifiessalmonellatyphimuriumtrafficandhostimmuneresponsesalongtheintestinaltract AT patrickmunro saccharomycesboulardiimodifiessalmonellatyphimuriumtrafficandhostimmuneresponsesalongtheintestinaltract AT laurentboyer saccharomycesboulardiimodifiessalmonellatyphimuriumtrafficandhostimmuneresponsesalongtheintestinaltract AT rodolpheanty saccharomycesboulardiimodifiessalmonellatyphimuriumtrafficandhostimmuneresponsesalongtheintestinaltract AT veroniqueimbert saccharomycesboulardiimodifiessalmonellatyphimuriumtrafficandhostimmuneresponsesalongtheintestinaltract AT chloeterciolo saccharomycesboulardiimodifiessalmonellatyphimuriumtrafficandhostimmuneresponsesalongtheintestinaltract AT fredericandre saccharomycesboulardiimodifiessalmonellatyphimuriumtrafficandhostimmuneresponsesalongtheintestinaltract AT patrickrampal saccharomycesboulardiimodifiessalmonellatyphimuriumtrafficandhostimmuneresponsesalongtheintestinaltract AT emmanuellemichez saccharomycesboulardiimodifiessalmonellatyphimuriumtrafficandhostimmuneresponsesalongtheintestinaltract AT jeanfrancoispeyron saccharomycesboulardiimodifiessalmonellatyphimuriumtrafficandhostimmuneresponsesalongtheintestinaltract AT dorotaczerucka saccharomycesboulardiimodifiessalmonellatyphimuriumtrafficandhostimmuneresponsesalongtheintestinaltract |
| _version_ |
1718414230023045120 |