Integrated immunoassay-based broad detection of multi-class mycotoxins

Europium (Eu3+) and horse radish peroxidase (HRP) are highly promising biosensors due to its excellent physical and chemical properties. The two biosensors are widely applied in time-resolved fluoroimmunoassay (TRFIA) and chemiluminescence enzyme-linked immunosorbent assay, respectively. In this wor...

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Autores principales:	Shupeng Yang, Xiujuan Yi, Xin Mao, Yunguo Liu, Suxia Zhang, Yanshen Li
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	pi–po multi-classes analysis integrated immunoassay biosensor Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/77aa5a460dda4c99a71850a7e84859e8
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spelling	oai:doaj.org-article:77aa5a460dda4c99a71850a7e84859e82021-11-26T11:19:46ZIntegrated immunoassay-based broad detection of multi-class mycotoxins0954-01051465-344310.1080/09540105.2018.1424121https://doaj.org/article/77aa5a460dda4c99a71850a7e84859e82018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1424121https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Europium (Eu3+) and horse radish peroxidase (HRP) are highly promising biosensors due to its excellent physical and chemical properties. The two biosensors are widely applied in time-resolved fluoroimmunoassay (TRFIA) and chemiluminescence enzyme-linked immunosorbent assay, respectively. In this work, the two technologies were integrated with parallel input and parallel output (PI–PO) characters. Eu3+ was adopted as probe I in TRFIA for deoxynivalenol and de-epoxyldexoylnivalenol with a limit of detection (LOD) of 4.74 and 4.56 ng/mL, while HRP was performed as probe II in CL-ELISA for FB1 with an LOD of 0.088 ng/mL. Compared to the traditional individual assay, this method exhibited fast and broad detection characteristics by integrating two immunoassays into one.Shupeng YangXiujuan YiXin MaoYunguo LiuSuxia ZhangYanshen LiTaylor & Francis Grouparticlepi–pomulti-classes analysisintegrated immunoassaybiosensorAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 615-624 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	pi–po multi-classes analysis integrated immunoassay biosensor Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	pi–po multi-classes analysis integrated immunoassay biosensor Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Shupeng Yang Xiujuan Yi Xin Mao Yunguo Liu Suxia Zhang Yanshen Li Integrated immunoassay-based broad detection of multi-class mycotoxins
description	Europium (Eu3+) and horse radish peroxidase (HRP) are highly promising biosensors due to its excellent physical and chemical properties. The two biosensors are widely applied in time-resolved fluoroimmunoassay (TRFIA) and chemiluminescence enzyme-linked immunosorbent assay, respectively. In this work, the two technologies were integrated with parallel input and parallel output (PI–PO) characters. Eu3+ was adopted as probe I in TRFIA for deoxynivalenol and de-epoxyldexoylnivalenol with a limit of detection (LOD) of 4.74 and 4.56 ng/mL, while HRP was performed as probe II in CL-ELISA for FB1 with an LOD of 0.088 ng/mL. Compared to the traditional individual assay, this method exhibited fast and broad detection characteristics by integrating two immunoassays into one.
format	article
author	Shupeng Yang Xiujuan Yi Xin Mao Yunguo Liu Suxia Zhang Yanshen Li
author_facet	Shupeng Yang Xiujuan Yi Xin Mao Yunguo Liu Suxia Zhang Yanshen Li
author_sort	Shupeng Yang
title	Integrated immunoassay-based broad detection of multi-class mycotoxins
title_short	Integrated immunoassay-based broad detection of multi-class mycotoxins
title_full	Integrated immunoassay-based broad detection of multi-class mycotoxins
title_fullStr	Integrated immunoassay-based broad detection of multi-class mycotoxins
title_full_unstemmed	Integrated immunoassay-based broad detection of multi-class mycotoxins
title_sort	integrated immunoassay-based broad detection of multi-class mycotoxins
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/77aa5a460dda4c99a71850a7e84859e8
work_keys_str_mv	AT shupengyang integratedimmunoassaybasedbroaddetectionofmulticlassmycotoxins AT xiujuanyi integratedimmunoassaybasedbroaddetectionofmulticlassmycotoxins AT xinmao integratedimmunoassaybasedbroaddetectionofmulticlassmycotoxins AT yunguoliu integratedimmunoassaybasedbroaddetectionofmulticlassmycotoxins AT suxiazhang integratedimmunoassaybasedbroaddetectionofmulticlassmycotoxins AT yanshenli integratedimmunoassaybasedbroaddetectionofmulticlassmycotoxins
_version_	1718409542884130816

Integrated immunoassay-based broad detection of multi-class mycotoxins

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