In vitro and in vivo Effects of a Single Dose of Bupivacaine 5% on Donkey Chondrocytes

Single intra-articular (IA) injection of long-acting local anesthetics such as bupivacaine is commonly used clinically for postoperative analgesia, in particular, after arthroscopic surgery. Despite their widespread use, the side effects of IA bupivacaine on joint cartilage as well as hepatotoxic an...

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Autores principales:	Kamal Hussein, Abdelbaset Eweda Abdelbaset, Ahmed Abdelrahiem Sadek, Ahmed Noreldin
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	bupivacaine local anesthetic intra-articular synovial fluid cartilage damage chondrolysis Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/77ab277acd5d400a9f944de14963fb5b
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spelling	oai:doaj.org-article:77ab277acd5d400a9f944de14963fb5b2021-11-30T12:03:47ZIn vitro and in vivo Effects of a Single Dose of Bupivacaine 5% on Donkey Chondrocytes2297-176910.3389/fvets.2021.661426https://doaj.org/article/77ab277acd5d400a9f944de14963fb5b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.661426/fullhttps://doaj.org/toc/2297-1769Single intra-articular (IA) injection of long-acting local anesthetics such as bupivacaine is commonly used clinically for postoperative analgesia, in particular, after arthroscopic surgery. Despite their widespread use, the side effects of IA bupivacaine on joint cartilage as well as hepatotoxic and nephrotoxic effects remain to be elucidated. The aim of this study is to assess the in vitro effect of bupivacaine 5% on donkey chondrocytes at different time points, in addition to the in vivo effects of a single IA bupivacaine injection on the middle carpal joint in a group of 10 clinically healthy adult male donkeys. In phase I, the effect of in vitro treatment with bupivacaine 5% or saline 0.9% on freshly isolated donkey chondrocytes for 30, 60 min, 24, 48, and 96 h was investigated using MTT and LIVE/DEAD assay. In phase II, in vivo effects of single injection of bupivacaine on the middle carpal joint of the donkey were evaluated compared with saline 0.9%. Biochemical analysis of collected serum and synovia was performed. Additionally, articular cartilage damage was evaluated using radiography, computed tomography (CT), catabolic marker expression via quantitative polymerase chain reaction (qPCR), and histopathological examination 96 h after injection. Our results showed that after a 30-min exposure to bupivacaine 5%, the viability of donkey chondrocytes was 97.3 ± 4.4% and was not significantly affected at the indicated time points (n = 8, p < 0.05). No significant changes in biochemical analytes of serum and synovial fluid following IA bupivacaine injection were observed, compared with saline injection (n = 5 for each group, p < 0.05). Furthermore, in vivo IA injection of bupivacaine revealed no significant differences in radiography, CT scan, gene expression of cartilage catabolic biomarkers, and histopathological examination. These results provide an evidence for the safety of bupivacaine on the donkey cartilage.Kamal HusseinAbdelbaset Eweda AbdelbasetAhmed Abdelrahiem SadekAhmed NoreldinFrontiers Media S.A.articlebupivacainelocal anestheticintra-articularsynovial fluidcartilage damagechondrolysisVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bupivacaine local anesthetic intra-articular synovial fluid cartilage damage chondrolysis Veterinary medicine SF600-1100
spellingShingle	bupivacaine local anesthetic intra-articular synovial fluid cartilage damage chondrolysis Veterinary medicine SF600-1100 Kamal Hussein Abdelbaset Eweda Abdelbaset Ahmed Abdelrahiem Sadek Ahmed Noreldin In vitro and in vivo Effects of a Single Dose of Bupivacaine 5% on Donkey Chondrocytes
description	Single intra-articular (IA) injection of long-acting local anesthetics such as bupivacaine is commonly used clinically for postoperative analgesia, in particular, after arthroscopic surgery. Despite their widespread use, the side effects of IA bupivacaine on joint cartilage as well as hepatotoxic and nephrotoxic effects remain to be elucidated. The aim of this study is to assess the in vitro effect of bupivacaine 5% on donkey chondrocytes at different time points, in addition to the in vivo effects of a single IA bupivacaine injection on the middle carpal joint in a group of 10 clinically healthy adult male donkeys. In phase I, the effect of in vitro treatment with bupivacaine 5% or saline 0.9% on freshly isolated donkey chondrocytes for 30, 60 min, 24, 48, and 96 h was investigated using MTT and LIVE/DEAD assay. In phase II, in vivo effects of single injection of bupivacaine on the middle carpal joint of the donkey were evaluated compared with saline 0.9%. Biochemical analysis of collected serum and synovia was performed. Additionally, articular cartilage damage was evaluated using radiography, computed tomography (CT), catabolic marker expression via quantitative polymerase chain reaction (qPCR), and histopathological examination 96 h after injection. Our results showed that after a 30-min exposure to bupivacaine 5%, the viability of donkey chondrocytes was 97.3 ± 4.4% and was not significantly affected at the indicated time points (n = 8, p < 0.05). No significant changes in biochemical analytes of serum and synovial fluid following IA bupivacaine injection were observed, compared with saline injection (n = 5 for each group, p < 0.05). Furthermore, in vivo IA injection of bupivacaine revealed no significant differences in radiography, CT scan, gene expression of cartilage catabolic biomarkers, and histopathological examination. These results provide an evidence for the safety of bupivacaine on the donkey cartilage.
format	article
author	Kamal Hussein Abdelbaset Eweda Abdelbaset Ahmed Abdelrahiem Sadek Ahmed Noreldin
author_facet	Kamal Hussein Abdelbaset Eweda Abdelbaset Ahmed Abdelrahiem Sadek Ahmed Noreldin
author_sort	Kamal Hussein
title	In vitro and in vivo Effects of a Single Dose of Bupivacaine 5% on Donkey Chondrocytes
title_short	In vitro and in vivo Effects of a Single Dose of Bupivacaine 5% on Donkey Chondrocytes
title_full	In vitro and in vivo Effects of a Single Dose of Bupivacaine 5% on Donkey Chondrocytes
title_fullStr	In vitro and in vivo Effects of a Single Dose of Bupivacaine 5% on Donkey Chondrocytes
title_full_unstemmed	In vitro and in vivo Effects of a Single Dose of Bupivacaine 5% on Donkey Chondrocytes
title_sort	in vitro and in vivo effects of a single dose of bupivacaine 5% on donkey chondrocytes
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/77ab277acd5d400a9f944de14963fb5b
work_keys_str_mv	AT kamalhussein invitroandinvivoeffectsofasingledoseofbupivacaine5ondonkeychondrocytes AT abdelbasetewedaabdelbaset invitroandinvivoeffectsofasingledoseofbupivacaine5ondonkeychondrocytes AT ahmedabdelrahiemsadek invitroandinvivoeffectsofasingledoseofbupivacaine5ondonkeychondrocytes AT ahmednoreldin invitroandinvivoeffectsofasingledoseofbupivacaine5ondonkeychondrocytes
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In vitro and in vivo Effects of a Single Dose of Bupivacaine 5% on Donkey Chondrocytes

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