Development of non-transgenic glyphosate tolerant wheat by TILLING.

Glyphosate (N-phosphonomethyl-glycine) is the world's most widely used broad spectrum, post-emergence herbicide. It inhibits the chloroplast-targeted enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19), a component of the plant and microorganism-specific shikimate pathway an...

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Autores principales:	Charles P Moehs, William J Austill, Daniel Facciotti, Aaron Holm, Dayna Loeffler, Zhongjin Lu, Jessica C Mullenberg, Ann J Slade, Michael N Steine, Jos van Boxtel, Cate McGuire
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/77ae5506e6fb48ffa274f68f80e87267
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spelling	oai:doaj.org-article:77ae5506e6fb48ffa274f68f80e872672021-12-02T20:06:16ZDevelopment of non-transgenic glyphosate tolerant wheat by TILLING.1932-620310.1371/journal.pone.0245802https://doaj.org/article/77ae5506e6fb48ffa274f68f80e872672021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0245802https://doaj.org/toc/1932-6203Glyphosate (N-phosphonomethyl-glycine) is the world's most widely used broad spectrum, post-emergence herbicide. It inhibits the chloroplast-targeted enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19), a component of the plant and microorganism-specific shikimate pathway and a key catalyst in the production of aromatic amino acids. Variants of EPSPS that are not inhibited by glyphosate due to particular amino acid alterations in the active site of the enzyme are known. Some of these variants have been identified in weed species that have developed resistance to glyphosate because of the strong selective pressure of continuous, heavy glyphosate use. We have used TILLING (Targeting Induced Local Lesions in Genomes), a non-transgenic, target-selected, reverse genetics, mutation breeding technique, and conventional genetic crosses, to identify and combine, through two rounds of mutagenesis, wheat lines having both T102I and P106S (so-called TIPS enzyme) mutations in both the A and the D sub-genome homoeologous copies of the wheat EPSPS gene. The combined effects of the T102I and P106S mutations are known from previous work in multiple species to minimize the binding of the herbicide while maintaining the affinity of the catalytic site for its native substrates. These novel wheat lines exhibit substantial tolerance to commercially relevant levels of glyphosate.Charles P MoehsWilliam J AustillDaniel FacciottiAaron HolmDayna LoefflerZhongjin LuJessica C MullenbergAnn J SladeMichael N SteineJos van BoxtelCate McGuirePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0245802 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charles P Moehs William J Austill Daniel Facciotti Aaron Holm Dayna Loeffler Zhongjin Lu Jessica C Mullenberg Ann J Slade Michael N Steine Jos van Boxtel Cate McGuire Development of non-transgenic glyphosate tolerant wheat by TILLING.
description	Glyphosate (N-phosphonomethyl-glycine) is the world's most widely used broad spectrum, post-emergence herbicide. It inhibits the chloroplast-targeted enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19), a component of the plant and microorganism-specific shikimate pathway and a key catalyst in the production of aromatic amino acids. Variants of EPSPS that are not inhibited by glyphosate due to particular amino acid alterations in the active site of the enzyme are known. Some of these variants have been identified in weed species that have developed resistance to glyphosate because of the strong selective pressure of continuous, heavy glyphosate use. We have used TILLING (Targeting Induced Local Lesions in Genomes), a non-transgenic, target-selected, reverse genetics, mutation breeding technique, and conventional genetic crosses, to identify and combine, through two rounds of mutagenesis, wheat lines having both T102I and P106S (so-called TIPS enzyme) mutations in both the A and the D sub-genome homoeologous copies of the wheat EPSPS gene. The combined effects of the T102I and P106S mutations are known from previous work in multiple species to minimize the binding of the herbicide while maintaining the affinity of the catalytic site for its native substrates. These novel wheat lines exhibit substantial tolerance to commercially relevant levels of glyphosate.
format	article
author	Charles P Moehs William J Austill Daniel Facciotti Aaron Holm Dayna Loeffler Zhongjin Lu Jessica C Mullenberg Ann J Slade Michael N Steine Jos van Boxtel Cate McGuire
author_facet	Charles P Moehs William J Austill Daniel Facciotti Aaron Holm Dayna Loeffler Zhongjin Lu Jessica C Mullenberg Ann J Slade Michael N Steine Jos van Boxtel Cate McGuire
author_sort	Charles P Moehs
title	Development of non-transgenic glyphosate tolerant wheat by TILLING.
title_short	Development of non-transgenic glyphosate tolerant wheat by TILLING.
title_full	Development of non-transgenic glyphosate tolerant wheat by TILLING.
title_fullStr	Development of non-transgenic glyphosate tolerant wheat by TILLING.
title_full_unstemmed	Development of non-transgenic glyphosate tolerant wheat by TILLING.
title_sort	development of non-transgenic glyphosate tolerant wheat by tilling.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/77ae5506e6fb48ffa274f68f80e87267
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Development of non-transgenic glyphosate tolerant wheat by TILLING.

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