Subcellular localization of nucleocapsid protein of SFTSV and its assembly into the ribonucleoprotein complex with L protein and viral RNA

Abstract Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging bunyavirus that causes novel zoonotic diseases in Asian countries including China, Japan, South Korea, and Vietnam. In phleboviruses, viral proteins play a critical role in viral particle formation inside the host cell...

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Autores principales:	Sithumini M. W. Lokupathirage, Yoshimi Tsuda, Kodai Ikegame, Kisho Noda, Devinda S. Muthusinghe, Fumiya Kozawa, Rashid Manzoor, Kenta Shimizu, Kumiko Yoshimatsu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/77ae9fdf21a746af8371d0313eebbd7c
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spelling	oai:doaj.org-article:77ae9fdf21a746af8371d0313eebbd7c2021-11-28T12:17:11ZSubcellular localization of nucleocapsid protein of SFTSV and its assembly into the ribonucleoprotein complex with L protein and viral RNA10.1038/s41598-021-01985-x2045-2322https://doaj.org/article/77ae9fdf21a746af8371d0313eebbd7c2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01985-xhttps://doaj.org/toc/2045-2322Abstract Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging bunyavirus that causes novel zoonotic diseases in Asian countries including China, Japan, South Korea, and Vietnam. In phleboviruses, viral proteins play a critical role in viral particle formation inside the host cells. Viral glycoproteins (GPs) and RNA-dependent RNA polymerase (RdRp) are colocalized in the Golgi apparatus and endoplasmic reticulum-Golgi intermediate compartment (ERGIC). The nucleocapsid (N) protein was widely expressed in the cytoplasm, even in cells coexpressing GP. However, the role of SFTSV N protein remains unclear. The subcellular localization of SFTSV structural proteins was investigated using a confocal microscope. Subsequently, minigenome and immunoprecipitation assays were carried out. The N protein interacts with viral RNA (vRNA) and further shows translational activity with RdRp which is L protein and localized in the ERGIC and Golgi apparatus when co-expressed with GP. On the other hand, mutant N protein did not interact with vRNA either localized in the ERGIC or Golgi apparatus. The interaction between the N protein of SFTSV and vRNA is important for the localization of viral proteins and viral assembly. This study provides useful insights into the life cycle of SFTSV, which will lead to the detection of antiviral targets.Sithumini M. W. LokupathirageYoshimi TsudaKodai IkegameKisho NodaDevinda S. MuthusingheFumiya KozawaRashid ManzoorKenta ShimizuKumiko YoshimatsuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sithumini M. W. Lokupathirage Yoshimi Tsuda Kodai Ikegame Kisho Noda Devinda S. Muthusinghe Fumiya Kozawa Rashid Manzoor Kenta Shimizu Kumiko Yoshimatsu Subcellular localization of nucleocapsid protein of SFTSV and its assembly into the ribonucleoprotein complex with L protein and viral RNA
description	Abstract Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging bunyavirus that causes novel zoonotic diseases in Asian countries including China, Japan, South Korea, and Vietnam. In phleboviruses, viral proteins play a critical role in viral particle formation inside the host cells. Viral glycoproteins (GPs) and RNA-dependent RNA polymerase (RdRp) are colocalized in the Golgi apparatus and endoplasmic reticulum-Golgi intermediate compartment (ERGIC). The nucleocapsid (N) protein was widely expressed in the cytoplasm, even in cells coexpressing GP. However, the role of SFTSV N protein remains unclear. The subcellular localization of SFTSV structural proteins was investigated using a confocal microscope. Subsequently, minigenome and immunoprecipitation assays were carried out. The N protein interacts with viral RNA (vRNA) and further shows translational activity with RdRp which is L protein and localized in the ERGIC and Golgi apparatus when co-expressed with GP. On the other hand, mutant N protein did not interact with vRNA either localized in the ERGIC or Golgi apparatus. The interaction between the N protein of SFTSV and vRNA is important for the localization of viral proteins and viral assembly. This study provides useful insights into the life cycle of SFTSV, which will lead to the detection of antiviral targets.
format	article
author	Sithumini M. W. Lokupathirage Yoshimi Tsuda Kodai Ikegame Kisho Noda Devinda S. Muthusinghe Fumiya Kozawa Rashid Manzoor Kenta Shimizu Kumiko Yoshimatsu
author_facet	Sithumini M. W. Lokupathirage Yoshimi Tsuda Kodai Ikegame Kisho Noda Devinda S. Muthusinghe Fumiya Kozawa Rashid Manzoor Kenta Shimizu Kumiko Yoshimatsu
author_sort	Sithumini M. W. Lokupathirage
title	Subcellular localization of nucleocapsid protein of SFTSV and its assembly into the ribonucleoprotein complex with L protein and viral RNA
title_short	Subcellular localization of nucleocapsid protein of SFTSV and its assembly into the ribonucleoprotein complex with L protein and viral RNA
title_full	Subcellular localization of nucleocapsid protein of SFTSV and its assembly into the ribonucleoprotein complex with L protein and viral RNA
title_fullStr	Subcellular localization of nucleocapsid protein of SFTSV and its assembly into the ribonucleoprotein complex with L protein and viral RNA
title_full_unstemmed	Subcellular localization of nucleocapsid protein of SFTSV and its assembly into the ribonucleoprotein complex with L protein and viral RNA
title_sort	subcellular localization of nucleocapsid protein of sftsv and its assembly into the ribonucleoprotein complex with l protein and viral rna
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/77ae9fdf21a746af8371d0313eebbd7c
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Subcellular localization of nucleocapsid protein of SFTSV and its assembly into the ribonucleoprotein complex with L protein and viral RNA

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