Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials
The synthesis of two biocatalysts based on a commercial <i>Candida antarctica</i> lipase B, CALB enzyme (E), physically immobilized on two silica supports, was carried out. The first support was a periodic mesoporous organosilica (PMO) and the second one was a commercial silica modified...
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MDPI AG
2021
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oai:doaj.org-article:77b5b84c2d174cf4b08cdd29624e57b62021-11-25T17:06:12ZEnzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials10.3390/catal111113502073-4344https://doaj.org/article/77b5b84c2d174cf4b08cdd29624e57b62021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4344/11/11/1350https://doaj.org/toc/2073-4344The synthesis of two biocatalysts based on a commercial <i>Candida antarctica</i> lipase B, CALB enzyme (E), physically immobilized on two silica supports, was carried out. The first support was a periodic mesoporous organosilica (PMO) and the second one was a commercial silica modified with octyl groups (octyl-MS3030). The maximum enzyme load was 122 mg enzyme/g support on PMO and 288 mg enzyme/g support on octyl-MS3030. In addition, the biocatalytic efficiency was corroborated by two reaction tests based on the hydrolysis of p-nitrophenylacetate (p-NPA) and tributyrin (TB). The transesterification of sunflower oil with ethanol was carried out over the biocatalysts synthesized at the following reaction conditions: 6 mL sunflower oil, 1.75 mL EtOH, 30 °C, 25 μL NaOH 10 N and 300 rpm, attaining conversion values over 80% after 3 h of reaction time. According to the results obtained, we can confirm that these biocatalytic systems are viable candidates to develop, optimize and improve a new methodology to achieve the integration of glycerol in different monoacylglycerol molecules together with fatty acid ethyl esters (FAEE) molecules to obtain Ecodiesel.Carlos LunaVictoria Gascón-PérezFrancisco J. López-TenlladoFelipa M. BautistaCristóbal Verdugo-EscamillaLaura Aguado-DeblasJuan CaleroAntonio A. RomeroDiego LunaRafael EstévezMDPI AGarticlebiofuelecodieselbiodieselcommercial CALB lipaseordered mesoporous materials (PMO)amorphous siliceous material MS3030Chemical technologyTP1-1185ChemistryQD1-999ENCatalysts, Vol 11, Iss 1350, p 1350 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
biofuel ecodiesel biodiesel commercial CALB lipase ordered mesoporous materials (PMO) amorphous siliceous material MS3030 Chemical technology TP1-1185 Chemistry QD1-999 |
| spellingShingle |
biofuel ecodiesel biodiesel commercial CALB lipase ordered mesoporous materials (PMO) amorphous siliceous material MS3030 Chemical technology TP1-1185 Chemistry QD1-999 Carlos Luna Victoria Gascón-Pérez Francisco J. López-Tenllado Felipa M. Bautista Cristóbal Verdugo-Escamilla Laura Aguado-Deblas Juan Calero Antonio A. Romero Diego Luna Rafael Estévez Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials |
| description |
The synthesis of two biocatalysts based on a commercial <i>Candida antarctica</i> lipase B, CALB enzyme (E), physically immobilized on two silica supports, was carried out. The first support was a periodic mesoporous organosilica (PMO) and the second one was a commercial silica modified with octyl groups (octyl-MS3030). The maximum enzyme load was 122 mg enzyme/g support on PMO and 288 mg enzyme/g support on octyl-MS3030. In addition, the biocatalytic efficiency was corroborated by two reaction tests based on the hydrolysis of p-nitrophenylacetate (p-NPA) and tributyrin (TB). The transesterification of sunflower oil with ethanol was carried out over the biocatalysts synthesized at the following reaction conditions: 6 mL sunflower oil, 1.75 mL EtOH, 30 °C, 25 μL NaOH 10 N and 300 rpm, attaining conversion values over 80% after 3 h of reaction time. According to the results obtained, we can confirm that these biocatalytic systems are viable candidates to develop, optimize and improve a new methodology to achieve the integration of glycerol in different monoacylglycerol molecules together with fatty acid ethyl esters (FAEE) molecules to obtain Ecodiesel. |
| format |
article |
| author |
Carlos Luna Victoria Gascón-Pérez Francisco J. López-Tenllado Felipa M. Bautista Cristóbal Verdugo-Escamilla Laura Aguado-Deblas Juan Calero Antonio A. Romero Diego Luna Rafael Estévez |
| author_facet |
Carlos Luna Victoria Gascón-Pérez Francisco J. López-Tenllado Felipa M. Bautista Cristóbal Verdugo-Escamilla Laura Aguado-Deblas Juan Calero Antonio A. Romero Diego Luna Rafael Estévez |
| author_sort |
Carlos Luna |
| title |
Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials |
| title_short |
Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials |
| title_full |
Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials |
| title_fullStr |
Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials |
| title_full_unstemmed |
Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials |
| title_sort |
enzymatic production of ecodiesel by using a commercial lipase calb, immobilized by physical adsorption on mesoporous organosilica materials |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/77b5b84c2d174cf4b08cdd29624e57b6 |
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