Strategic single point mutation yields a solvent- and salt-stable transaminase from Virgibacillus sp. in soluble form

Abstract A new transaminase (VbTA) was identified from the genome of the halotolerant marine bacterium Virgibacillus 21D. Following heterologous expression in Escherichia coli, it was located entirely in the insoluble fraction. After a single mutation, identified via sequence homology analyses, the...

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Autores principales: Benedetta Guidi, Matteo Planchestainer, Martina Letizia Contente, Tommaso Laurenzi, Ivano Eberini, Louise J. Gourlay, Diego Romano, Francesca Paradisi, Francesco Molinari
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/77bae478128847fb89ddb990cced95fd
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