Proteomics Analysis of Serum Proteins in Gestational Diabetes

Objective. The purpose of this study was to screen serum proteins for biomarkers of gestational diabetes mellitus (GDM) and to investigate its pathogenesis by analyzing the differences in serum proteomics between pregnant women with GDM and healthy pregnant women. Methods. Patients who were admitted...

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Autores principales:	Jianhua Li, Lin Lu, Xinping Xie, Xiaofeng Dai, Shan Zheng, Lihong Chen
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/77bc2c5f3fd94bcd82b6708b323925d1
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spelling	oai:doaj.org-article:77bc2c5f3fd94bcd82b6708b323925d12021-11-15T01:20:12ZProteomics Analysis of Serum Proteins in Gestational Diabetes1741-428810.1155/2021/4724590https://doaj.org/article/77bc2c5f3fd94bcd82b6708b323925d12021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/4724590https://doaj.org/toc/1741-4288Objective. The purpose of this study was to screen serum proteins for biomarkers of gestational diabetes mellitus (GDM) and to investigate its pathogenesis by analyzing the differences in serum proteomics between pregnant women with GDM and healthy pregnant women. Methods. Patients who were admitted to the First Affiliated Hospital of Fujian Medical University from June 2019 to January 2020 were included. According to the medical history and the results of the 75 g oral glucose tolerance test (OGTT), they were divided into the normal pregnant women group and GDM pregnant women group. The serum of two groups of patients was collected. High performance liquid chromatography-mass spectrometry was used to identify differentially expressed serum proteins between pregnant women with GDM and healthy pregnant women, and bioinformatics analysis was then performed on the identified proteins. Results. A total of 1152 quantifiable proteins were detected; among them, 15 were upregulated in serum of GDM pregnant women, while 26 were downregulated. The subsequent parallel reaction monitoring (PRM) assay validated the expression levels of 12 out of 41 differentially expressed proteins. Moreover, bioinformatics analysis revealed that the differentially expressed proteins are involved in multiple biological processes and signaling pathways related to the lipid metabolism, glycan degradation, immune response, and platelet aggregation. Conclusions. This study identified 41 serum proteins with differential expression between pregnant women with GDM and healthy pregnant women, providing new candidate molecules for elucidating GDM pathogenesis and screening therapeutic targets.Jianhua LiLin LuXinping XieXiaofeng DaiShan ZhengLihong ChenHindawi LimitedarticleOther systems of medicineRZ201-999ENEvidence-Based Complementary and Alternative Medicine, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Other systems of medicine RZ201-999
spellingShingle	Other systems of medicine RZ201-999 Jianhua Li Lin Lu Xinping Xie Xiaofeng Dai Shan Zheng Lihong Chen Proteomics Analysis of Serum Proteins in Gestational Diabetes
description	Objective. The purpose of this study was to screen serum proteins for biomarkers of gestational diabetes mellitus (GDM) and to investigate its pathogenesis by analyzing the differences in serum proteomics between pregnant women with GDM and healthy pregnant women. Methods. Patients who were admitted to the First Affiliated Hospital of Fujian Medical University from June 2019 to January 2020 were included. According to the medical history and the results of the 75 g oral glucose tolerance test (OGTT), they were divided into the normal pregnant women group and GDM pregnant women group. The serum of two groups of patients was collected. High performance liquid chromatography-mass spectrometry was used to identify differentially expressed serum proteins between pregnant women with GDM and healthy pregnant women, and bioinformatics analysis was then performed on the identified proteins. Results. A total of 1152 quantifiable proteins were detected; among them, 15 were upregulated in serum of GDM pregnant women, while 26 were downregulated. The subsequent parallel reaction monitoring (PRM) assay validated the expression levels of 12 out of 41 differentially expressed proteins. Moreover, bioinformatics analysis revealed that the differentially expressed proteins are involved in multiple biological processes and signaling pathways related to the lipid metabolism, glycan degradation, immune response, and platelet aggregation. Conclusions. This study identified 41 serum proteins with differential expression between pregnant women with GDM and healthy pregnant women, providing new candidate molecules for elucidating GDM pathogenesis and screening therapeutic targets.
format	article
author	Jianhua Li Lin Lu Xinping Xie Xiaofeng Dai Shan Zheng Lihong Chen
author_facet	Jianhua Li Lin Lu Xinping Xie Xiaofeng Dai Shan Zheng Lihong Chen
author_sort	Jianhua Li
title	Proteomics Analysis of Serum Proteins in Gestational Diabetes
title_short	Proteomics Analysis of Serum Proteins in Gestational Diabetes
title_full	Proteomics Analysis of Serum Proteins in Gestational Diabetes
title_fullStr	Proteomics Analysis of Serum Proteins in Gestational Diabetes
title_full_unstemmed	Proteomics Analysis of Serum Proteins in Gestational Diabetes
title_sort	proteomics analysis of serum proteins in gestational diabetes
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/77bc2c5f3fd94bcd82b6708b323925d1
work_keys_str_mv	AT jianhuali proteomicsanalysisofserumproteinsingestationaldiabetes AT linlu proteomicsanalysisofserumproteinsingestationaldiabetes AT xinpingxie proteomicsanalysisofserumproteinsingestationaldiabetes AT xiaofengdai proteomicsanalysisofserumproteinsingestationaldiabetes AT shanzheng proteomicsanalysisofserumproteinsingestationaldiabetes AT lihongchen proteomicsanalysisofserumproteinsingestationaldiabetes
_version_	1718428873145712640

Proteomics Analysis of Serum Proteins in Gestational Diabetes

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