Formulation, Cellular Uptake and Cytotoxicity of Thymoquinone-Loaded PLGA Nanoparticles in Malignant Melanoma Cancer Cells

Wisam Nabeel Ibrahim,1,2 Luqman Muizzuddin Bin Mohd Rosli,2 Abd Almonem Doolaanea2 1Department of Biomedical Sciences, College of Health Sciences, QU Health, Qatar University, Doha, Qatar; 2Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, Kuant...

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Autores principales:	Ibrahim WN, Muizzuddin Bin Mohd Rosli L, Doolaanea AA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	microencapsulation thymoquinone plga nanoparticles melanoma Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/77bf430e7f884049823c910fedb905d5
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spelling	oai:doaj.org-article:77bf430e7f884049823c910fedb905d52021-12-02T11:07:47ZFormulation, Cellular Uptake and Cytotoxicity of Thymoquinone-Loaded PLGA Nanoparticles in Malignant Melanoma Cancer Cells1178-2013https://doaj.org/article/77bf430e7f884049823c910fedb905d52020-10-01T00:00:00Zhttps://www.dovepress.com/formulation-cellular-uptake-and-cytotoxicity-of-thymoquinone-loaded-pl-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wisam Nabeel Ibrahim,1,2 Luqman Muizzuddin Bin Mohd Rosli,2 Abd Almonem Doolaanea2 1Department of Biomedical Sciences, College of Health Sciences, QU Health, Qatar University, Doha, Qatar; 2Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, Kuantan 25200, MalaysiaCorrespondence: Wisam Nabeel Ibrahim Tel +97444036025Email w.ibrahim@qu.edu.qa; Abd Almonem Doolaanea Tel +6013-6238628Email abdalmonemdoolaanea@yahoo.comIntroduction: Thymoquinone (TQ) is the main active compound extracted from Nigella sativa a traditional herb with wide therapeutic applications and recognizable anticancer properties. This study aimed to formulate and characterize TQ-nanoparticles using PLGA as a biocompatible coating material (TQ-PLGA NPs) with the evaluation of its therapeutic properties in human melanoma cancer cells.Methods: The TQ-PLGA NPs were prepared and characterized for size, zeta potential, encapsulation efficiency, and release profile.Results: The particle size was 147.2 nm, with 22.1 positive zeta potential and 96.8% encapsulation efficiency. The NPs released 45.6% of the encapsulated TQ within 3 h followed by characteristic sustained release over 7 days with a total of 69.7% cumulative release. TQ-PLGA NPs were taken up effectively by the cells in a time-dependent manner up to 24 h. Higher cell toxicity was determined within the first 24 h in melanoma cells due to the rapid release of TQ from the NPs and its low stability in the cell culture media.Conclusion: TQ-PLGA NPs is a potential anticancer agent taking advantage of the sustained release and tailored size that allows accumulation in the cancer tissue by the enhanced permeability and retention effect. However, stability problems of the active ingredient were address in this study and requires further investigation.Keywords: microencapsulation, thymoquinone, PLGA, nanoparticles, melanomaIbrahim WNMuizzuddin Bin Mohd Rosli LDoolaanea AADove Medical PressarticlemicroencapsulationthymoquinoneplgananoparticlesmelanomaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 8059-8074 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	microencapsulation thymoquinone plga nanoparticles melanoma Medicine (General) R5-920
spellingShingle	microencapsulation thymoquinone plga nanoparticles melanoma Medicine (General) R5-920 Ibrahim WN Muizzuddin Bin Mohd Rosli L Doolaanea AA Formulation, Cellular Uptake and Cytotoxicity of Thymoquinone-Loaded PLGA Nanoparticles in Malignant Melanoma Cancer Cells
description	Wisam Nabeel Ibrahim,1,2 Luqman Muizzuddin Bin Mohd Rosli,2 Abd Almonem Doolaanea2 1Department of Biomedical Sciences, College of Health Sciences, QU Health, Qatar University, Doha, Qatar; 2Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, Kuantan 25200, MalaysiaCorrespondence: Wisam Nabeel Ibrahim Tel +97444036025Email w.ibrahim@qu.edu.qa; Abd Almonem Doolaanea Tel +6013-6238628Email abdalmonemdoolaanea@yahoo.comIntroduction: Thymoquinone (TQ) is the main active compound extracted from Nigella sativa a traditional herb with wide therapeutic applications and recognizable anticancer properties. This study aimed to formulate and characterize TQ-nanoparticles using PLGA as a biocompatible coating material (TQ-PLGA NPs) with the evaluation of its therapeutic properties in human melanoma cancer cells.Methods: The TQ-PLGA NPs were prepared and characterized for size, zeta potential, encapsulation efficiency, and release profile.Results: The particle size was 147.2 nm, with 22.1 positive zeta potential and 96.8% encapsulation efficiency. The NPs released 45.6% of the encapsulated TQ within 3 h followed by characteristic sustained release over 7 days with a total of 69.7% cumulative release. TQ-PLGA NPs were taken up effectively by the cells in a time-dependent manner up to 24 h. Higher cell toxicity was determined within the first 24 h in melanoma cells due to the rapid release of TQ from the NPs and its low stability in the cell culture media.Conclusion: TQ-PLGA NPs is a potential anticancer agent taking advantage of the sustained release and tailored size that allows accumulation in the cancer tissue by the enhanced permeability and retention effect. However, stability problems of the active ingredient were address in this study and requires further investigation.Keywords: microencapsulation, thymoquinone, PLGA, nanoparticles, melanoma
format	article
author	Ibrahim WN Muizzuddin Bin Mohd Rosli L Doolaanea AA
author_facet	Ibrahim WN Muizzuddin Bin Mohd Rosli L Doolaanea AA
author_sort	Ibrahim WN
title	Formulation, Cellular Uptake and Cytotoxicity of Thymoquinone-Loaded PLGA Nanoparticles in Malignant Melanoma Cancer Cells
title_short	Formulation, Cellular Uptake and Cytotoxicity of Thymoquinone-Loaded PLGA Nanoparticles in Malignant Melanoma Cancer Cells
title_full	Formulation, Cellular Uptake and Cytotoxicity of Thymoquinone-Loaded PLGA Nanoparticles in Malignant Melanoma Cancer Cells
title_fullStr	Formulation, Cellular Uptake and Cytotoxicity of Thymoquinone-Loaded PLGA Nanoparticles in Malignant Melanoma Cancer Cells
title_full_unstemmed	Formulation, Cellular Uptake and Cytotoxicity of Thymoquinone-Loaded PLGA Nanoparticles in Malignant Melanoma Cancer Cells
title_sort	formulation, cellular uptake and cytotoxicity of thymoquinone-loaded plga nanoparticles in malignant melanoma cancer cells
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/77bf430e7f884049823c910fedb905d5
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Formulation, Cellular Uptake and Cytotoxicity of Thymoquinone-Loaded PLGA Nanoparticles in Malignant Melanoma Cancer Cells

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