Application of Bacteriophages on Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) Biofilm

Shiga toxin-producing <i>Escherichia coli</i> are pathogenic bacteria able to form biofilms both on abiotic surfaces and on food, thus increasing risks for food consumers. Moreover, biofilms are difficult to remove and more resistant to antimicrobial agents compared to planktonic cells....

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Autores principales:	Nicola Mangieri, Roberto Foschino, Claudia Picozzi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biofilm bacteriophages Shiga toxin-producing <i>Escherichia coli</i> (STEC) biocontrol Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/77bf56db23664a41927477fd588b2476
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spelling	oai:doaj.org-article:77bf56db23664a41927477fd588b24762021-11-25T16:25:12ZApplication of Bacteriophages on Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) Biofilm10.3390/antibiotics101114232079-6382https://doaj.org/article/77bf56db23664a41927477fd588b24762021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1423https://doaj.org/toc/2079-6382Shiga toxin-producing <i>Escherichia coli</i> are pathogenic bacteria able to form biofilms both on abiotic surfaces and on food, thus increasing risks for food consumers. Moreover, biofilms are difficult to remove and more resistant to antimicrobial agents compared to planktonic cells. Bacteriophages, natural predators of bacteria, can be used as an alternative to prevent biofilm formation or to remove pre-formed biofilm. In this work, four STEC able to produce biofilm were selected among 31 different strains and tested against single bacteriophages and two-phage cocktails. Results showed that our phages were able to reduce biofilm formation by 43.46% both when used as single phage preparation and as a cocktail formulation. Since one of the two cocktails had a slightly better performance, it was used to remove pre-existing biofilms. In this case, the phages were unable to destroy the biofilms and reduce the number of bacterial cells. Our data confirm that preventing biofilm formation in a food plant is better than trying to remove a preformed biofilm and the continuous presence of bacteriophages in the process environment could reduce the number of bacteria able to form biofilms and therefore improve the food safety.Nicola MangieriRoberto FoschinoClaudia PicozziMDPI AGarticlebiofilmbacteriophagesShiga toxin-producing <i>Escherichia coli</i> (STEC)biocontrolTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1423, p 1423 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biofilm bacteriophages Shiga toxin-producing <i>Escherichia coli</i> (STEC) biocontrol Therapeutics. Pharmacology RM1-950
spellingShingle	biofilm bacteriophages Shiga toxin-producing <i>Escherichia coli</i> (STEC) biocontrol Therapeutics. Pharmacology RM1-950 Nicola Mangieri Roberto Foschino Claudia Picozzi Application of Bacteriophages on Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) Biofilm
description	Shiga toxin-producing <i>Escherichia coli</i> are pathogenic bacteria able to form biofilms both on abiotic surfaces and on food, thus increasing risks for food consumers. Moreover, biofilms are difficult to remove and more resistant to antimicrobial agents compared to planktonic cells. Bacteriophages, natural predators of bacteria, can be used as an alternative to prevent biofilm formation or to remove pre-formed biofilm. In this work, four STEC able to produce biofilm were selected among 31 different strains and tested against single bacteriophages and two-phage cocktails. Results showed that our phages were able to reduce biofilm formation by 43.46% both when used as single phage preparation and as a cocktail formulation. Since one of the two cocktails had a slightly better performance, it was used to remove pre-existing biofilms. In this case, the phages were unable to destroy the biofilms and reduce the number of bacterial cells. Our data confirm that preventing biofilm formation in a food plant is better than trying to remove a preformed biofilm and the continuous presence of bacteriophages in the process environment could reduce the number of bacteria able to form biofilms and therefore improve the food safety.
format	article
author	Nicola Mangieri Roberto Foschino Claudia Picozzi
author_facet	Nicola Mangieri Roberto Foschino Claudia Picozzi
author_sort	Nicola Mangieri
title	Application of Bacteriophages on Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) Biofilm
title_short	Application of Bacteriophages on Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) Biofilm
title_full	Application of Bacteriophages on Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) Biofilm
title_fullStr	Application of Bacteriophages on Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) Biofilm
title_full_unstemmed	Application of Bacteriophages on Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) Biofilm
title_sort	application of bacteriophages on shiga toxin-producing <i>escherichia coli</i> (stec) biofilm
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/77bf56db23664a41927477fd588b2476
work_keys_str_mv	AT nicolamangieri applicationofbacteriophagesonshigatoxinproducingiescherichiacoliistecbiofilm AT robertofoschino applicationofbacteriophagesonshigatoxinproducingiescherichiacoliistecbiofilm AT claudiapicozzi applicationofbacteriophagesonshigatoxinproducingiescherichiacoliistecbiofilm
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Application of Bacteriophages on Shiga Toxin-Producing <i>Escherichia coli</i> (STEC) Biofilm

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