ROR1 and ROR2 expression in pancreatic cancer

Abstract Background The Wnt receptors ROR1 and ROR2 are generating increased interest as cancer therapeutic targets but remain understudied in pancreatic ductal adenocarcinoma (PDAC). Compared to canonical Wnt/ β-catenin signalling, the role of noncanonical Wnt signalling in PDAC remains largely unk...

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Autores principales:	Dongli Liu, George Sharbeen, Phoebe Phillips, Australian Pancreatic Cancer Genome Initiative, Caroline E. Ford
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/77c1d36dfb864511964d2a94105709b4
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spelling	oai:doaj.org-article:77c1d36dfb864511964d2a94105709b42021-11-14T12:30:17ZROR1 and ROR2 expression in pancreatic cancer10.1186/s12885-021-08952-91471-2407https://doaj.org/article/77c1d36dfb864511964d2a94105709b42021-11-01T00:00:00Zhttps://doi.org/10.1186/s12885-021-08952-9https://doaj.org/toc/1471-2407Abstract Background The Wnt receptors ROR1 and ROR2 are generating increased interest as cancer therapeutic targets but remain understudied in pancreatic ductal adenocarcinoma (PDAC). Compared to canonical Wnt/ β-catenin signalling, the role of noncanonical Wnt signalling in PDAC remains largely unknown. Only one study has investigated the prognostic significance of the noncanonical Wnt signalling receptor, ROR2 in PDAC. No studies have investigated the prognostic role of ROR1 in PDAC. Methods Here, we performed analysis of ROR1 and ROR2 mRNA expression in three publicly available datasets ICGC-PACA-AU (n = 81), TCGA-PAAD (n = 150) and CPTAC-PDAC (n = 137). ROR1 and ROR2 protein expression from the CPTAC-PDAC discovery cohort were also analysed. Immunohistochemistry (IHC) using the validated anti ROR1 monoclonal antibody (4A5) was performed on the Australian Pancreatic Cancer Genome Initiative (APGI) cohort of PDAC samples (n = 152). Association between ROR1 cytoplasmic staining intensity and clinicopathological parameters including stage, grade and overall survival (OS) was investigated. Results High ROR1 mRNA expression levels correlated with a favourable OS outcome in all of the ICGC-PACA-AU, TCGA-PAAD and CPTAC-PDAC cohorts. ROR1 protein expression was not associated with stage, grade or OS in the APGI cohort. Conclusion ROR1 and ROR2 have potential as prognostic markers when measured at the mRNA level in PDAC. Our IHC cohort did not support ROR1 protein expression in predicting OS, and highlighted the discrepancy of prognostic biomarkers when measured by MS, IHC and RNAseq.Dongli LiuGeorge SharbeenPhoebe PhillipsAustralian Pancreatic Cancer Genome InitiativeCaroline E. FordBMCarticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBMC Cancer, Vol 21, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Dongli Liu George Sharbeen Phoebe Phillips Australian Pancreatic Cancer Genome Initiative Caroline E. Ford ROR1 and ROR2 expression in pancreatic cancer
description	Abstract Background The Wnt receptors ROR1 and ROR2 are generating increased interest as cancer therapeutic targets but remain understudied in pancreatic ductal adenocarcinoma (PDAC). Compared to canonical Wnt/ β-catenin signalling, the role of noncanonical Wnt signalling in PDAC remains largely unknown. Only one study has investigated the prognostic significance of the noncanonical Wnt signalling receptor, ROR2 in PDAC. No studies have investigated the prognostic role of ROR1 in PDAC. Methods Here, we performed analysis of ROR1 and ROR2 mRNA expression in three publicly available datasets ICGC-PACA-AU (n = 81), TCGA-PAAD (n = 150) and CPTAC-PDAC (n = 137). ROR1 and ROR2 protein expression from the CPTAC-PDAC discovery cohort were also analysed. Immunohistochemistry (IHC) using the validated anti ROR1 monoclonal antibody (4A5) was performed on the Australian Pancreatic Cancer Genome Initiative (APGI) cohort of PDAC samples (n = 152). Association between ROR1 cytoplasmic staining intensity and clinicopathological parameters including stage, grade and overall survival (OS) was investigated. Results High ROR1 mRNA expression levels correlated with a favourable OS outcome in all of the ICGC-PACA-AU, TCGA-PAAD and CPTAC-PDAC cohorts. ROR1 protein expression was not associated with stage, grade or OS in the APGI cohort. Conclusion ROR1 and ROR2 have potential as prognostic markers when measured at the mRNA level in PDAC. Our IHC cohort did not support ROR1 protein expression in predicting OS, and highlighted the discrepancy of prognostic biomarkers when measured by MS, IHC and RNAseq.
format	article
author	Dongli Liu George Sharbeen Phoebe Phillips Australian Pancreatic Cancer Genome Initiative Caroline E. Ford
author_facet	Dongli Liu George Sharbeen Phoebe Phillips Australian Pancreatic Cancer Genome Initiative Caroline E. Ford
author_sort	Dongli Liu
title	ROR1 and ROR2 expression in pancreatic cancer
title_short	ROR1 and ROR2 expression in pancreatic cancer
title_full	ROR1 and ROR2 expression in pancreatic cancer
title_fullStr	ROR1 and ROR2 expression in pancreatic cancer
title_full_unstemmed	ROR1 and ROR2 expression in pancreatic cancer
title_sort	ror1 and ror2 expression in pancreatic cancer
publisher	BMC
publishDate	2021
url	https://doaj.org/article/77c1d36dfb864511964d2a94105709b4
work_keys_str_mv	AT dongliliu ror1andror2expressioninpancreaticcancer AT georgesharbeen ror1andror2expressioninpancreaticcancer AT phoebephillips ror1andror2expressioninpancreaticcancer AT australianpancreaticcancergenomeinitiative ror1andror2expressioninpancreaticcancer AT carolineeford ror1andror2expressioninpancreaticcancer
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ROR1 and ROR2 expression in pancreatic cancer

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