Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland

Abstract Preventive infusion of antibiotics in the mammary gland of cows consumes 11 tons/year of medically relevant antimicrobials, yet, this practice might not be critical to prevent new infections in the healthy mammary gland of cows. Here, we used next-generation sequencing and quantitative real...

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Autores principales:	Erika C. R. Bonsaglia, Marilia S. Gomes, Igor F. Canisso, Ziyao Zhou, Svetlana F. Lima, Vera L. M. Rall, Georgios Oikonomou, Rodrigo C. Bicalho, Fabio S. Lima
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/77c1f77966354089a3abd75fca1b0f6d
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spelling	oai:doaj.org-article:77c1f77966354089a3abd75fca1b0f6d2021-12-02T16:07:57ZMilk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland10.1038/s41598-017-08790-52045-2322https://doaj.org/article/77c1f77966354089a3abd75fca1b0f6d2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08790-5https://doaj.org/toc/2045-2322Abstract Preventive infusion of antibiotics in the mammary gland of cows consumes 11 tons/year of medically relevant antimicrobials, yet, this practice might not be critical to prevent new infections in the healthy mammary gland of cows. Here, we used next-generation sequencing and quantitative real-time PCR to determine the impact of dry cow therapy without antibiotics on milk microbiome and bacterial load, respectively. Cows diagnosed as negative for mastitis at dry off were randomly allocated to receive antibiotic (intramammary ceftiofur hydrochloride) and teat sealant or just teat sealant. Firmicutes was the most abundant phylum, and Corynebacterium, Acinetobacter, and Staphylococcus, often involved in mastitis cases, were the most abundant genera across treatments and time. However, there were no effects of antimicrobial on milk microbiome and bacterial load. Bacterial load was greater at seven days postpartum than at dry off. Dry cow therapy based on teat sealant without antibiotics can be used with no detrimental impacts on milk microbiome and bacterial load in cows with a healthy mammary gland.Erika C. R. BonsagliaMarilia S. GomesIgor F. CanissoZiyao ZhouSvetlana F. LimaVera L. M. RallGeorgios OikonomouRodrigo C. BicalhoFabio S. LimaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Erika C. R. Bonsaglia Marilia S. Gomes Igor F. Canisso Ziyao Zhou Svetlana F. Lima Vera L. M. Rall Georgios Oikonomou Rodrigo C. Bicalho Fabio S. Lima Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland
description	Abstract Preventive infusion of antibiotics in the mammary gland of cows consumes 11 tons/year of medically relevant antimicrobials, yet, this practice might not be critical to prevent new infections in the healthy mammary gland of cows. Here, we used next-generation sequencing and quantitative real-time PCR to determine the impact of dry cow therapy without antibiotics on milk microbiome and bacterial load, respectively. Cows diagnosed as negative for mastitis at dry off were randomly allocated to receive antibiotic (intramammary ceftiofur hydrochloride) and teat sealant or just teat sealant. Firmicutes was the most abundant phylum, and Corynebacterium, Acinetobacter, and Staphylococcus, often involved in mastitis cases, were the most abundant genera across treatments and time. However, there were no effects of antimicrobial on milk microbiome and bacterial load. Bacterial load was greater at seven days postpartum than at dry off. Dry cow therapy based on teat sealant without antibiotics can be used with no detrimental impacts on milk microbiome and bacterial load in cows with a healthy mammary gland.
format	article
author	Erika C. R. Bonsaglia Marilia S. Gomes Igor F. Canisso Ziyao Zhou Svetlana F. Lima Vera L. M. Rall Georgios Oikonomou Rodrigo C. Bicalho Fabio S. Lima
author_facet	Erika C. R. Bonsaglia Marilia S. Gomes Igor F. Canisso Ziyao Zhou Svetlana F. Lima Vera L. M. Rall Georgios Oikonomou Rodrigo C. Bicalho Fabio S. Lima
author_sort	Erika C. R. Bonsaglia
title	Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland
title_short	Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland
title_full	Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland
title_fullStr	Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland
title_full_unstemmed	Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland
title_sort	milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/77c1f77966354089a3abd75fca1b0f6d
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Milk microbiome and bacterial load following dry cow therapy without antibiotics in dairy cows with healthy mammary gland

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