SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells

Abstract Pyroptosis is a kind of necrotic and inflammatory programmed cell death induced by inflammatory caspases. SENP7 is a SUMO-specific protease, which mainly acts on deconjugation of SUMOs from substrate proteins. We evaluated the effect of SENP7 knockdown on pyroptosis, NF-κB signaling pathway...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xun Li, Fangzhou Jiao, Jia Hong, Fan Yang, Luwen Wang, Zuojiong Gong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/77c5462dc7c84befaa83d6f423c30f10
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:77c5462dc7c84befaa83d6f423c30f10
record_format	dspace
spelling	oai:doaj.org-article:77c5462dc7c84befaa83d6f423c30f102021-12-02T18:51:07ZSENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells10.1038/s41598-020-73400-w2045-2322https://doaj.org/article/77c5462dc7c84befaa83d6f423c30f102020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-73400-whttps://doaj.org/toc/2045-2322Abstract Pyroptosis is a kind of necrotic and inflammatory programmed cell death induced by inflammatory caspases. SENP7 is a SUMO-specific protease, which mainly acts on deconjugation of SUMOs from substrate proteins. We evaluated the effect of SENP7 knockdown on pyroptosis, NF-κB signaling pathway, and NLRP3 inflammasome in Raw 264.7 cells. The results showed that the GSDMD protein mainly expressed in the cytoplasm nearby nuclei of Raw 264.7 cells. It migrated to cytomembrane with the numbers of Raw 264.7 cell decreased when LPS + ATP were administrated. Which was inhibited by SENP7 knockdown. In addition, not only the pyroptosis of Raw 264.7 cells was inhibited, the activation of NF-κB signaling pathway and NLRP3 inflammasome were also attenuated by SENP7 knockdown. The mechanism may be associated with the over SUMOylation of proteins induced by SENP7 knockdown.Xun LiFangzhou JiaoJia HongFan YangLuwen WangZuojiong GongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xun Li Fangzhou Jiao Jia Hong Fan Yang Luwen Wang Zuojiong Gong SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells
description	Abstract Pyroptosis is a kind of necrotic and inflammatory programmed cell death induced by inflammatory caspases. SENP7 is a SUMO-specific protease, which mainly acts on deconjugation of SUMOs from substrate proteins. We evaluated the effect of SENP7 knockdown on pyroptosis, NF-κB signaling pathway, and NLRP3 inflammasome in Raw 264.7 cells. The results showed that the GSDMD protein mainly expressed in the cytoplasm nearby nuclei of Raw 264.7 cells. It migrated to cytomembrane with the numbers of Raw 264.7 cell decreased when LPS + ATP were administrated. Which was inhibited by SENP7 knockdown. In addition, not only the pyroptosis of Raw 264.7 cells was inhibited, the activation of NF-κB signaling pathway and NLRP3 inflammasome were also attenuated by SENP7 knockdown. The mechanism may be associated with the over SUMOylation of proteins induced by SENP7 knockdown.
format	article
author	Xun Li Fangzhou Jiao Jia Hong Fan Yang Luwen Wang Zuojiong Gong
author_facet	Xun Li Fangzhou Jiao Jia Hong Fan Yang Luwen Wang Zuojiong Gong
author_sort	Xun Li
title	SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells
title_short	SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells
title_full	SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells
title_fullStr	SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells
title_full_unstemmed	SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells
title_sort	senp7 knockdown inhibited pyroptosis and nf-κb/nlrp3 inflammasome pathway activation in raw 264.7 cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/77c5462dc7c84befaa83d6f423c30f10
work_keys_str_mv	AT xunli senp7knockdowninhibitedpyroptosisandnfkbnlrp3inflammasomepathwayactivationinraw2647cells AT fangzhoujiao senp7knockdowninhibitedpyroptosisandnfkbnlrp3inflammasomepathwayactivationinraw2647cells AT jiahong senp7knockdowninhibitedpyroptosisandnfkbnlrp3inflammasomepathwayactivationinraw2647cells AT fanyang senp7knockdowninhibitedpyroptosisandnfkbnlrp3inflammasomepathwayactivationinraw2647cells AT luwenwang senp7knockdowninhibitedpyroptosisandnfkbnlrp3inflammasomepathwayactivationinraw2647cells AT zuojionggong senp7knockdowninhibitedpyroptosisandnfkbnlrp3inflammasomepathwayactivationinraw2647cells
_version_	1718377454922366976

SENP7 knockdown inhibited pyroptosis and NF-κB/NLRP3 inflammasome pathway activation in Raw 264.7 cells

Ejemplares similares