The relation between oral Candida load and bacterial microbiome profiles in Dutch older adults.

Currently there are no evidence-based ecological measures for prevention of overgrowth and subsequent infection by fungi in the oral cavity. The aim of this study was to increase our knowledge on fungal-bacterial ecological interactions. Salivary Candida abundance of 82 Dutch adults aged 58-80 years...

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Autores principales:	Eefje A Kraneveld, Mark J Buijs, Marc J Bonder, Marjolein Visser, Bart J F Keijser, Wim Crielaard, Egija Zaura
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/77c57f83678b44b282515324956e34a2
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spelling	oai:doaj.org-article:77c57f83678b44b282515324956e34a22021-11-18T07:09:06ZThe relation between oral Candida load and bacterial microbiome profiles in Dutch older adults.1932-620310.1371/journal.pone.0042770https://doaj.org/article/77c57f83678b44b282515324956e34a22012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22900048/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Currently there are no evidence-based ecological measures for prevention of overgrowth and subsequent infection by fungi in the oral cavity. The aim of this study was to increase our knowledge on fungal-bacterial ecological interactions. Salivary Candida abundance of 82 Dutch adults aged 58-80 years was established relative to the bacterial load by quantitative PCR analysis of the Internal Transcribed (ITS) region (Candida) and 16S rDNA gene (bacteria). The salivary microbiome was assessed using barcoded pyrosequencing of the bacterial hypervariable regions V5-V7 of 16S rDNA. Sequencing data was preprocessed by denoising and chimera removal, clustered in Operational Taxonomic Units (OTUs) and assigned to taxonomy. Both OTU-based (PCA, diversity statistics) and phylogeny-based analyses (UniFrac, PCoA) were performed. Saliva of Dutch older adults contained 0-4 × 10(8) CFU/mL Candida with a median Candida load of 0.06%. With increased Candida load the diversity of the salivary microbiome decreased significantly (p<0.001). Increase in the Candida load correlated positively with class Bacilli, and negatively with class Fusobacteria, Flavobacteria, and Bacteroidia. Microbiomes with high Candida load were less diverse and had a distinct microbial composition towards dominance by saccharolytic and acidogenic bacteria--streptococci. The control of the acidification of the oral environment may be a potential preventive measure for Candida outgrowth that should be evaluated in longitudinal clinical intervention trials.Eefje A KraneveldMark J BuijsMarc J BonderMarjolein VisserBart J F KeijserWim CrielaardEgija ZauraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42770 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eefje A Kraneveld Mark J Buijs Marc J Bonder Marjolein Visser Bart J F Keijser Wim Crielaard Egija Zaura The relation between oral Candida load and bacterial microbiome profiles in Dutch older adults.
description	Currently there are no evidence-based ecological measures for prevention of overgrowth and subsequent infection by fungi in the oral cavity. The aim of this study was to increase our knowledge on fungal-bacterial ecological interactions. Salivary Candida abundance of 82 Dutch adults aged 58-80 years was established relative to the bacterial load by quantitative PCR analysis of the Internal Transcribed (ITS) region (Candida) and 16S rDNA gene (bacteria). The salivary microbiome was assessed using barcoded pyrosequencing of the bacterial hypervariable regions V5-V7 of 16S rDNA. Sequencing data was preprocessed by denoising and chimera removal, clustered in Operational Taxonomic Units (OTUs) and assigned to taxonomy. Both OTU-based (PCA, diversity statistics) and phylogeny-based analyses (UniFrac, PCoA) were performed. Saliva of Dutch older adults contained 0-4 × 10(8) CFU/mL Candida with a median Candida load of 0.06%. With increased Candida load the diversity of the salivary microbiome decreased significantly (p<0.001). Increase in the Candida load correlated positively with class Bacilli, and negatively with class Fusobacteria, Flavobacteria, and Bacteroidia. Microbiomes with high Candida load were less diverse and had a distinct microbial composition towards dominance by saccharolytic and acidogenic bacteria--streptococci. The control of the acidification of the oral environment may be a potential preventive measure for Candida outgrowth that should be evaluated in longitudinal clinical intervention trials.
format	article
author	Eefje A Kraneveld Mark J Buijs Marc J Bonder Marjolein Visser Bart J F Keijser Wim Crielaard Egija Zaura
author_facet	Eefje A Kraneveld Mark J Buijs Marc J Bonder Marjolein Visser Bart J F Keijser Wim Crielaard Egija Zaura
author_sort	Eefje A Kraneveld
title	The relation between oral Candida load and bacterial microbiome profiles in Dutch older adults.
title_short	The relation between oral Candida load and bacterial microbiome profiles in Dutch older adults.
title_full	The relation between oral Candida load and bacterial microbiome profiles in Dutch older adults.
title_fullStr	The relation between oral Candida load and bacterial microbiome profiles in Dutch older adults.
title_full_unstemmed	The relation between oral Candida load and bacterial microbiome profiles in Dutch older adults.
title_sort	relation between oral candida load and bacterial microbiome profiles in dutch older adults.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/77c57f83678b44b282515324956e34a2
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The relation between oral Candida load and bacterial microbiome profiles in Dutch older adults.

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