GENETIC ANALYSIS OF HOKKAIDO HANTAVIRUS AMONG MYODES RUFOCANUS IN THE BAIKAL LAKE AREA

Hokkaido hantavirus (HOKV) identified originally in the grey red-backed vole (Myodes rufocanus) in Hokkaido, Japan. Subsequent studies showed different genetic lineages of HOKV in Sakhalin, Buryatia and Far Eastern regions of Russia and in China. Tissuesfrom 68 arvicolid rodents, captured in regions...

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Autores principales:	L. N. Yashina, G. A. Danchinova, S. V. Seregin, M. A. Khasnatinov, R. Yanagihara
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2013
Materias:	hantavirus hokkaido myodes rufocanus russia Science Q
Acceso en línea:	https://doaj.org/article/77cae3c9bc224626b3b0dcaf9acc3cb1
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spelling	oai:doaj.org-article:77cae3c9bc224626b3b0dcaf9acc3cb12021-11-23T06:14:29ZGENETIC ANALYSIS OF HOKKAIDO HANTAVIRUS AMONG MYODES RUFOCANUS IN THE BAIKAL LAKE AREA2541-94202587-9596https://doaj.org/article/77cae3c9bc224626b3b0dcaf9acc3cb12013-07-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/1621https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Hokkaido hantavirus (HOKV) identified originally in the grey red-backed vole (Myodes rufocanus) in Hokkaido, Japan. Subsequent studies showed different genetic lineages of HOKV in Sakhalin, Buryatia and Far Eastern regions of Russia and in China. Tissuesfrom 68 arvicolid rodents, captured in regions south and west of Baikal Lake, were initially tested for hantaviral antigen by ELISA, and tissues from antigen-positive rodents were analyzed for hantavirus RNA by RT-PCr. Taxonomic identification of host species was based on phylogenetic analysis of partial cytochrome b gene sequences. Hantavirus Land S-segment sequences were detected in two antigen-positive M. rufocanus, from the Tunka region of Buryatia Republic (south side) and the Olhon region of Irkutsk Oblast (west side). Sequence analysis showed that the newfound hantavirus strains, designated Baikal and Siberia, represented genetic variants of HOKV Previously unknown genetic variant designated Siberia was identified in M. rufocanus captured in Olhon region. Second genetic variant from Tunka region, designated Baikal, was closely related to previously described hantavirus strain from the same region. Alignment and comparison of the nucleotide and amino acid sequences showed intra-strain differences of 18,4 % and 5,3 % for the L segment and 17,4 % and 3,5 % for the S segment, respectively. Sequence divergence from geographically distant HOKV strains were 17,4-21,5 % and 3,9-6,8 % for the L segment and 15,2-17,0 % and 3,3-4,0 % for the S segment, respectively. Phylogenetic analysis, based on a 346-nucleotide region of the L segment, revealed four lineages represented by previously reported variants from Japan and Sakhalin (strains Kitahiyama128L/2008, Tobetsu35L/2010 and Sakhalin99L/1998), Shkotovo in Far-Eastern Russia (strain Khekhtsir37L/2002) and the new variants from Baikal Lake. Analysis of the N protein, coding by the S segment, identified specific amino acid signatures for YJRV of Lys5, Arg26, Val/Ile68, Val/Ala9 He262, Pro283. Conclusions: HOKV is widespread across the geographic range of its arvicolid rodent reservoir host.L. N. YashinaG. A. DanchinovaS. V. SereginM. A. KhasnatinovR. YanagiharaScientific Сentre for Family Health and Human Reproduction Problemsarticlehantavirushokkaidomyodes rufocanusrussiaScienceQRUActa Biomedica Scientifica, Vol 0, Iss 4, Pp 147-152 (2013)
institution	DOAJ
collection	DOAJ
language	RU
topic	hantavirus hokkaido myodes rufocanus russia Science Q
spellingShingle	hantavirus hokkaido myodes rufocanus russia Science Q L. N. Yashina G. A. Danchinova S. V. Seregin M. A. Khasnatinov R. Yanagihara GENETIC ANALYSIS OF HOKKAIDO HANTAVIRUS AMONG MYODES RUFOCANUS IN THE BAIKAL LAKE AREA
description	Hokkaido hantavirus (HOKV) identified originally in the grey red-backed vole (Myodes rufocanus) in Hokkaido, Japan. Subsequent studies showed different genetic lineages of HOKV in Sakhalin, Buryatia and Far Eastern regions of Russia and in China. Tissuesfrom 68 arvicolid rodents, captured in regions south and west of Baikal Lake, were initially tested for hantaviral antigen by ELISA, and tissues from antigen-positive rodents were analyzed for hantavirus RNA by RT-PCr. Taxonomic identification of host species was based on phylogenetic analysis of partial cytochrome b gene sequences. Hantavirus Land S-segment sequences were detected in two antigen-positive M. rufocanus, from the Tunka region of Buryatia Republic (south side) and the Olhon region of Irkutsk Oblast (west side). Sequence analysis showed that the newfound hantavirus strains, designated Baikal and Siberia, represented genetic variants of HOKV Previously unknown genetic variant designated Siberia was identified in M. rufocanus captured in Olhon region. Second genetic variant from Tunka region, designated Baikal, was closely related to previously described hantavirus strain from the same region. Alignment and comparison of the nucleotide and amino acid sequences showed intra-strain differences of 18,4 % and 5,3 % for the L segment and 17,4 % and 3,5 % for the S segment, respectively. Sequence divergence from geographically distant HOKV strains were 17,4-21,5 % and 3,9-6,8 % for the L segment and 15,2-17,0 % and 3,3-4,0 % for the S segment, respectively. Phylogenetic analysis, based on a 346-nucleotide region of the L segment, revealed four lineages represented by previously reported variants from Japan and Sakhalin (strains Kitahiyama128L/2008, Tobetsu35L/2010 and Sakhalin99L/1998), Shkotovo in Far-Eastern Russia (strain Khekhtsir37L/2002) and the new variants from Baikal Lake. Analysis of the N protein, coding by the S segment, identified specific amino acid signatures for YJRV of Lys5, Arg26, Val/Ile68, Val/Ala9 He262, Pro283. Conclusions: HOKV is widespread across the geographic range of its arvicolid rodent reservoir host.
format	article
author	L. N. Yashina G. A. Danchinova S. V. Seregin M. A. Khasnatinov R. Yanagihara
author_facet	L. N. Yashina G. A. Danchinova S. V. Seregin M. A. Khasnatinov R. Yanagihara
author_sort	L. N. Yashina
title	GENETIC ANALYSIS OF HOKKAIDO HANTAVIRUS AMONG MYODES RUFOCANUS IN THE BAIKAL LAKE AREA
title_short	GENETIC ANALYSIS OF HOKKAIDO HANTAVIRUS AMONG MYODES RUFOCANUS IN THE BAIKAL LAKE AREA
title_full	GENETIC ANALYSIS OF HOKKAIDO HANTAVIRUS AMONG MYODES RUFOCANUS IN THE BAIKAL LAKE AREA
title_fullStr	GENETIC ANALYSIS OF HOKKAIDO HANTAVIRUS AMONG MYODES RUFOCANUS IN THE BAIKAL LAKE AREA
title_full_unstemmed	GENETIC ANALYSIS OF HOKKAIDO HANTAVIRUS AMONG MYODES RUFOCANUS IN THE BAIKAL LAKE AREA
title_sort	genetic analysis of hokkaido hantavirus among myodes rufocanus in the baikal lake area
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2013
url	https://doaj.org/article/77cae3c9bc224626b3b0dcaf9acc3cb1
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GENETIC ANALYSIS OF HOKKAIDO HANTAVIRUS AMONG MYODES RUFOCANUS IN THE BAIKAL LAKE AREA

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