Antifungal activity of soybean rhizosphere actinomycetes producing bioactive compounds against Fusarium oxysporum

Mariastuti HD, Listiyowati S, Wahyudi AT. 2018. Antifungal activity of soybean rhizosphere actinomycetes producing bioactive compounds against Fusarium oxysporum. Biodiversitas 19: 2127-2133. Actinomycetes are known as the most potential source bioactive compounds. This research aimed to explore the...

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Autores principales:	HIMA DEWI MARIASTUTI, SRI LISTIYOWATI, ARIS TRI WAHYUDI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	antifungal activity bioactive compounds gc-ms rhizosphere actinomycetes Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/77d2092500f843c6b2870ba9d5f0ec09
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spelling	oai:doaj.org-article:77d2092500f843c6b2870ba9d5f0ec092021-11-16T14:04:40ZAntifungal activity of soybean rhizosphere actinomycetes producing bioactive compounds against Fusarium oxysporum1412-033X2085-472210.13057/biodiv/d190619https://doaj.org/article/77d2092500f843c6b2870ba9d5f0ec092018-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3093https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Mariastuti HD, Listiyowati S, Wahyudi AT. 2018. Antifungal activity of soybean rhizosphere actinomycetes producing bioactive compounds against Fusarium oxysporum. Biodiversitas 19: 2127-2133. Actinomycetes are known as the most potential source bioactive compounds. This research aimed to explore the potential of actinomycetes isolated from rhizosphere of soybean in antifungal activity against Fusarium oxysporum, a pathogen causing damping off, wilt, and root rot in soybean. Of 18 actinomycetes isolates tested, 9 isolates had antifungal activity against F. oxysporum in different inhibition percentages ranging from 38.69% to 73.50%. The lowest and the highest inhibition percentage were performed by ARK 63 and ARK 94 respectively. As tested by compatibility assay, all of isolates were compatible toward Bradyrhizobium japonicum, a symbiotic bacteria which colonized the soybean root confirmed by no clear zone formed in the agar medium. The activity of 9 isolates was confirmed in sterile and nonsterile soil toward in planta test. All of these isolates exhibited the consistent antifungal activity both in vitro and in planta. The diseases suppression in sterile soil was ranging from 36% to 80.7% while in non-sterile soil was ranging from 67% to 100%. The highest disease suppression in sterile soil was performed by ARK 17 while in non-sterile soil was performed by ARK 92 and ARK 94. Supporting the results, 5 crude extracts of the potential isolates also showed the antifungal activity with inhibition percentage ranging from 19% to 54.09%. The highest inhibition percentage was performed by ARK 94. ARK 94 showed consistent inhibition against F. oxysporum in vitro, in planta, and also in crude extract assay. Chemical composition of crude extract derived from ARK 94 was identified by gas chromatography-mass spectrometry (GC-MS) analysis. Among 12 major compounds, 7 compounds act as antifungal based on some references. Based on those potential characters, this isolate is potential to be further developed as biocontrol agent.HIMA DEWI MARIASTUTISRI LISTIYOWATIARIS TRI WAHYUDIMBI & UNS Soloarticleantifungal activitybioactive compoundsgc-msrhizosphere actinomycetesBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 6, Pp 2127-2133 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	antifungal activity bioactive compounds gc-ms rhizosphere actinomycetes Biology (General) QH301-705.5
spellingShingle	antifungal activity bioactive compounds gc-ms rhizosphere actinomycetes Biology (General) QH301-705.5 HIMA DEWI MARIASTUTI SRI LISTIYOWATI ARIS TRI WAHYUDI Antifungal activity of soybean rhizosphere actinomycetes producing bioactive compounds against Fusarium oxysporum
description	Mariastuti HD, Listiyowati S, Wahyudi AT. 2018. Antifungal activity of soybean rhizosphere actinomycetes producing bioactive compounds against Fusarium oxysporum. Biodiversitas 19: 2127-2133. Actinomycetes are known as the most potential source bioactive compounds. This research aimed to explore the potential of actinomycetes isolated from rhizosphere of soybean in antifungal activity against Fusarium oxysporum, a pathogen causing damping off, wilt, and root rot in soybean. Of 18 actinomycetes isolates tested, 9 isolates had antifungal activity against F. oxysporum in different inhibition percentages ranging from 38.69% to 73.50%. The lowest and the highest inhibition percentage were performed by ARK 63 and ARK 94 respectively. As tested by compatibility assay, all of isolates were compatible toward Bradyrhizobium japonicum, a symbiotic bacteria which colonized the soybean root confirmed by no clear zone formed in the agar medium. The activity of 9 isolates was confirmed in sterile and nonsterile soil toward in planta test. All of these isolates exhibited the consistent antifungal activity both in vitro and in planta. The diseases suppression in sterile soil was ranging from 36% to 80.7% while in non-sterile soil was ranging from 67% to 100%. The highest disease suppression in sterile soil was performed by ARK 17 while in non-sterile soil was performed by ARK 92 and ARK 94. Supporting the results, 5 crude extracts of the potential isolates also showed the antifungal activity with inhibition percentage ranging from 19% to 54.09%. The highest inhibition percentage was performed by ARK 94. ARK 94 showed consistent inhibition against F. oxysporum in vitro, in planta, and also in crude extract assay. Chemical composition of crude extract derived from ARK 94 was identified by gas chromatography-mass spectrometry (GC-MS) analysis. Among 12 major compounds, 7 compounds act as antifungal based on some references. Based on those potential characters, this isolate is potential to be further developed as biocontrol agent.
format	article
author	HIMA DEWI MARIASTUTI SRI LISTIYOWATI ARIS TRI WAHYUDI
author_facet	HIMA DEWI MARIASTUTI SRI LISTIYOWATI ARIS TRI WAHYUDI
author_sort	HIMA DEWI MARIASTUTI
title	Antifungal activity of soybean rhizosphere actinomycetes producing bioactive compounds against Fusarium oxysporum
title_short	Antifungal activity of soybean rhizosphere actinomycetes producing bioactive compounds against Fusarium oxysporum
title_full	Antifungal activity of soybean rhizosphere actinomycetes producing bioactive compounds against Fusarium oxysporum
title_fullStr	Antifungal activity of soybean rhizosphere actinomycetes producing bioactive compounds against Fusarium oxysporum
title_full_unstemmed	Antifungal activity of soybean rhizosphere actinomycetes producing bioactive compounds against Fusarium oxysporum
title_sort	antifungal activity of soybean rhizosphere actinomycetes producing bioactive compounds against fusarium oxysporum
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/77d2092500f843c6b2870ba9d5f0ec09
work_keys_str_mv	AT himadewimariastuti antifungalactivityofsoybeanrhizosphereactinomycetesproducingbioactivecompoundsagainstfusariumoxysporum AT srilistiyowati antifungalactivityofsoybeanrhizosphereactinomycetesproducingbioactivecompoundsagainstfusariumoxysporum AT aristriwahyudi antifungalactivityofsoybeanrhizosphereactinomycetesproducingbioactivecompoundsagainstfusariumoxysporum
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Antifungal activity of soybean rhizosphere actinomycetes producing bioactive compounds against Fusarium oxysporum

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