The Intriguing Conundrum of a Nonconserved Multifunctional Protein of Citrus Tristeza Virus That Interacts with a Viral Long Non-Coding RNA

<i>Citrus tristeza virus</i> (CTV), the largest non-segmented plant RNA virus, has several peculiar features, among which is the production of a 5′-terminal long non-coding RNA (lncRNA) termed low-molecular-weight tristeza 1 (LMT1). In this study, we found that p33, a unique viral protei...

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Autores principales:	Sung-Hwan Kang, Vicken Aknadibossian, Laxmi Kharel, Shachinthaka D. Dissanayaka Mudiyanselage, Ying Wang, Svetlana Y. Folimonova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	RNA virus closterovirus citrus tristeza virus long non-coding RNA plant virus RNA-binding protein Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/77dab480f57e467db5703b5d2b706604
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spelling	oai:doaj.org-article:77dab480f57e467db5703b5d2b7066042021-11-25T19:12:32ZThe Intriguing Conundrum of a Nonconserved Multifunctional Protein of Citrus Tristeza Virus That Interacts with a Viral Long Non-Coding RNA10.3390/v131121291999-4915https://doaj.org/article/77dab480f57e467db5703b5d2b7066042021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2129https://doaj.org/toc/1999-4915<i>Citrus tristeza virus</i> (CTV), the largest non-segmented plant RNA virus, has several peculiar features, among which is the production of a 5′-terminal long non-coding RNA (lncRNA) termed low-molecular-weight tristeza 1 (LMT1). In this study, we found that p33, a unique viral protein that performs multiple functions in the virus infection cycle, specifically binds LMT1, both in vivo and in vitro. These results were obtained through the expression of p33 under the context of the wild type virus infection or along with a mutant CTV variant that does not produce LMT1 as well as via ectopic co-expression of p33 with LMT1 in <i>Nicotiana benthamiana</i> leaves followed by RNA immunoprecipitation and rapid amplification of cDNA ends assays. Further experiments in which a recombinant p33 protein and an in vitro transcribed full-length LMT1 RNA or its truncated fragments were subjected to an electrophoretic mobility shift assay demonstrated that p33 binds to at least two distinct regions within LMT1. To the best of our knowledge, this is the first report of a plant virus protein binding to a lncRNA produced by the same virus. The biological significance of the interaction between these two viral factors is discussed.Sung-Hwan KangVicken AknadibossianLaxmi KharelShachinthaka D. Dissanayaka MudiyanselageYing WangSvetlana Y. FolimonovaMDPI AGarticleRNA virusclosteroviruscitrus tristeza viruslong non-coding RNAplant virusRNA-binding proteinMicrobiologyQR1-502ENViruses, Vol 13, Iss 2129, p 2129 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	RNA virus closterovirus citrus tristeza virus long non-coding RNA plant virus RNA-binding protein Microbiology QR1-502
spellingShingle	RNA virus closterovirus citrus tristeza virus long non-coding RNA plant virus RNA-binding protein Microbiology QR1-502 Sung-Hwan Kang Vicken Aknadibossian Laxmi Kharel Shachinthaka D. Dissanayaka Mudiyanselage Ying Wang Svetlana Y. Folimonova The Intriguing Conundrum of a Nonconserved Multifunctional Protein of Citrus Tristeza Virus That Interacts with a Viral Long Non-Coding RNA
description	<i>Citrus tristeza virus</i> (CTV), the largest non-segmented plant RNA virus, has several peculiar features, among which is the production of a 5′-terminal long non-coding RNA (lncRNA) termed low-molecular-weight tristeza 1 (LMT1). In this study, we found that p33, a unique viral protein that performs multiple functions in the virus infection cycle, specifically binds LMT1, both in vivo and in vitro. These results were obtained through the expression of p33 under the context of the wild type virus infection or along with a mutant CTV variant that does not produce LMT1 as well as via ectopic co-expression of p33 with LMT1 in <i>Nicotiana benthamiana</i> leaves followed by RNA immunoprecipitation and rapid amplification of cDNA ends assays. Further experiments in which a recombinant p33 protein and an in vitro transcribed full-length LMT1 RNA or its truncated fragments were subjected to an electrophoretic mobility shift assay demonstrated that p33 binds to at least two distinct regions within LMT1. To the best of our knowledge, this is the first report of a plant virus protein binding to a lncRNA produced by the same virus. The biological significance of the interaction between these two viral factors is discussed.
format	article
author	Sung-Hwan Kang Vicken Aknadibossian Laxmi Kharel Shachinthaka D. Dissanayaka Mudiyanselage Ying Wang Svetlana Y. Folimonova
author_facet	Sung-Hwan Kang Vicken Aknadibossian Laxmi Kharel Shachinthaka D. Dissanayaka Mudiyanselage Ying Wang Svetlana Y. Folimonova
author_sort	Sung-Hwan Kang
title	The Intriguing Conundrum of a Nonconserved Multifunctional Protein of Citrus Tristeza Virus That Interacts with a Viral Long Non-Coding RNA
title_short	The Intriguing Conundrum of a Nonconserved Multifunctional Protein of Citrus Tristeza Virus That Interacts with a Viral Long Non-Coding RNA
title_full	The Intriguing Conundrum of a Nonconserved Multifunctional Protein of Citrus Tristeza Virus That Interacts with a Viral Long Non-Coding RNA
title_fullStr	The Intriguing Conundrum of a Nonconserved Multifunctional Protein of Citrus Tristeza Virus That Interacts with a Viral Long Non-Coding RNA
title_full_unstemmed	The Intriguing Conundrum of a Nonconserved Multifunctional Protein of Citrus Tristeza Virus That Interacts with a Viral Long Non-Coding RNA
title_sort	intriguing conundrum of a nonconserved multifunctional protein of citrus tristeza virus that interacts with a viral long non-coding rna
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/77dab480f57e467db5703b5d2b706604
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The Intriguing Conundrum of a Nonconserved Multifunctional Protein of Citrus Tristeza Virus That Interacts with a Viral Long Non-Coding RNA

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