Identification and function of myeloid differentiation factor 88 (MyD88) in Litopenaeus vannamei.

Myeloid differentiation factor 88 (MyD88) is a universal and essential signaling protein in Toll-like receptor/interleukin-1 receptor-induced activation of nuclear factor-kappa B. In this study, two MyD88 protein variants (LvMyD88 and LvMyD88-1) were identified in Litopenaeus vannamei. The LvMyD88 c...

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Autores principales:	Shuang Zhang, Chao-Zheng Li, Hui Yan, Wei Qiu, Yong-Gui Chen, Pei-Hui Wang, Shao-Ping Weng, Jian-Guo He
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/77e67521eced4278a197c03aab6efdc1
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spelling	oai:doaj.org-article:77e67521eced4278a197c03aab6efdc12021-11-18T08:12:12ZIdentification and function of myeloid differentiation factor 88 (MyD88) in Litopenaeus vannamei.1932-620310.1371/journal.pone.0047038https://doaj.org/article/77e67521eced4278a197c03aab6efdc12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23071706/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Myeloid differentiation factor 88 (MyD88) is a universal and essential signaling protein in Toll-like receptor/interleukin-1 receptor-induced activation of nuclear factor-kappa B. In this study, two MyD88 protein variants (LvMyD88 and LvMyD88-1) were identified in Litopenaeus vannamei. The LvMyD88 cDNA is 1,848 bp in length and contains an open reading frame (ORF) of 1,428 bp, whereas the LvMyD88-1 cDNA is 1,719 bp in length and has an ORF of 1,299 bp. Both variants encode proteins with death and Toll/interleukin-1 receptor domains and share 91% sequence identity. In healthy L. vannamei, the LvMyD88 genes were highly expressed in hemocytes but at a low level in the hepatopancreas. The LvMyD88s expression was induced in hemocytes after challenge with lipopolysaccharide, CpG-ODN2006, Vibrio parahaemolyticus, Staphyloccocus aureus, and white spot syndrome virus, but not by poly I∶C. Overexpression of LvMyD88 and LvMyD88-1 in Drosophila Schneider 2 cells led to activation of antimicrobial peptide genes and wsv069 (ie1), wsv303, and wsv371. These results suggested that LvMyD88 may play a role in antibacterial and antiviral response in L. vannamei. To our knowledge, this is the first report on MyD88 in shrimp and a variant of MyD88 gene in invertebrates.Shuang ZhangChao-Zheng LiHui YanWei QiuYong-Gui ChenPei-Hui WangShao-Ping WengJian-Guo HePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e47038 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuang Zhang Chao-Zheng Li Hui Yan Wei Qiu Yong-Gui Chen Pei-Hui Wang Shao-Ping Weng Jian-Guo He Identification and function of myeloid differentiation factor 88 (MyD88) in Litopenaeus vannamei.
description	Myeloid differentiation factor 88 (MyD88) is a universal and essential signaling protein in Toll-like receptor/interleukin-1 receptor-induced activation of nuclear factor-kappa B. In this study, two MyD88 protein variants (LvMyD88 and LvMyD88-1) were identified in Litopenaeus vannamei. The LvMyD88 cDNA is 1,848 bp in length and contains an open reading frame (ORF) of 1,428 bp, whereas the LvMyD88-1 cDNA is 1,719 bp in length and has an ORF of 1,299 bp. Both variants encode proteins with death and Toll/interleukin-1 receptor domains and share 91% sequence identity. In healthy L. vannamei, the LvMyD88 genes were highly expressed in hemocytes but at a low level in the hepatopancreas. The LvMyD88s expression was induced in hemocytes after challenge with lipopolysaccharide, CpG-ODN2006, Vibrio parahaemolyticus, Staphyloccocus aureus, and white spot syndrome virus, but not by poly I∶C. Overexpression of LvMyD88 and LvMyD88-1 in Drosophila Schneider 2 cells led to activation of antimicrobial peptide genes and wsv069 (ie1), wsv303, and wsv371. These results suggested that LvMyD88 may play a role in antibacterial and antiviral response in L. vannamei. To our knowledge, this is the first report on MyD88 in shrimp and a variant of MyD88 gene in invertebrates.
format	article
author	Shuang Zhang Chao-Zheng Li Hui Yan Wei Qiu Yong-Gui Chen Pei-Hui Wang Shao-Ping Weng Jian-Guo He
author_facet	Shuang Zhang Chao-Zheng Li Hui Yan Wei Qiu Yong-Gui Chen Pei-Hui Wang Shao-Ping Weng Jian-Guo He
author_sort	Shuang Zhang
title	Identification and function of myeloid differentiation factor 88 (MyD88) in Litopenaeus vannamei.
title_short	Identification and function of myeloid differentiation factor 88 (MyD88) in Litopenaeus vannamei.
title_full	Identification and function of myeloid differentiation factor 88 (MyD88) in Litopenaeus vannamei.
title_fullStr	Identification and function of myeloid differentiation factor 88 (MyD88) in Litopenaeus vannamei.
title_full_unstemmed	Identification and function of myeloid differentiation factor 88 (MyD88) in Litopenaeus vannamei.
title_sort	identification and function of myeloid differentiation factor 88 (myd88) in litopenaeus vannamei.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/77e67521eced4278a197c03aab6efdc1
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Identification and function of myeloid differentiation factor 88 (MyD88) in Litopenaeus vannamei.

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