Isolation and purification of glycoglycerolipids to induce apoptosis in breast cancer cells

Abstract Monogalactosyldiacylglycerol (MGDG) is the most abundant type of glycoglycerolipid found in the plant cell membrane and mostly in the chloroplast thylakoid membrane. The amphiphilic nature of MGDG is attractive in pharmaceutical fields for interaction with other biological molecules and hen...

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Autores principales:	Muhammad Raisul Abedin, Sutapa Barua
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/77ed7f9e0d134a6facd16479c6af8752
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spelling	oai:doaj.org-article:77ed7f9e0d134a6facd16479c6af87522021-12-02T14:01:35ZIsolation and purification of glycoglycerolipids to induce apoptosis in breast cancer cells10.1038/s41598-020-80484-x2045-2322https://doaj.org/article/77ed7f9e0d134a6facd16479c6af87522021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80484-xhttps://doaj.org/toc/2045-2322Abstract Monogalactosyldiacylglycerol (MGDG) is the most abundant type of glycoglycerolipid found in the plant cell membrane and mostly in the chloroplast thylakoid membrane. The amphiphilic nature of MGDG is attractive in pharmaceutical fields for interaction with other biological molecules and hence exerting therapeutic anti-cancer, anti-viral, and anti-inflammatory activities. In this study, we investigated the therapeutic efficacy of cyanobacteria derived MGDG to inhibit breast cancer cell growth. MGDG was extracted from a cyanobacteria Synechocystis sp. PCC 6803 followed by a subsequent fractionation by column chromatographic technique. The purity and molecular structure of MGDG were analyzed by nuclear magnetic resonance (NMR) spectroscopy analysis. The presence of MGDG in the extracted fraction was further confirmed and quantified by high-performance liquid chromatography (HPLC). The anti-proliferation activity of the extracted MGDG molecule was tested against BT-474 and MDA-MB-231 breast cancer cell lines. The in vitro study showed that MGDG extracted from Synechocystis sp. PCC 6803 induced apoptosis in (70 ± 8) % of BT-474 (p < 0.001) and (58 ± 5) % of MDA-MB-231 cells (p < 0.001) using ~ 60 and 200 ng/ml of concentrations, respectively. The half-maximal inhibitory concentration, IC50 of MGDG extracted from Synechocystis sp. PCC 6803 were (27.2 ± 7.6) and (150 ± 70) ng/ml in BT-474 and MDA-MB-231 cell lines, respectively. Quantification of caspase-3/7 activity using flow cytometry showed (3.0 ± 0.4) and (2.1 ± 0.04)-fold (p < 0.001) higher protein expressions in the MGDG treated BT-474 and MDA-MB-231 cells, respectively than untreated controls conferring to the caspase-dependent apoptosis. The MGDG did not show any significant cytotoxic side effects in human dermal fibroblasts cells. A commercially available MGDG control did not induce any apoptotic cell death in cancer cells substantiating the potential of the MGDG extracted from Synechocystis sp. PCC 6803 for the treatment of breast cancer cells through the apoptosis-mediated pathway.Muhammad Raisul AbedinSutapa BaruaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muhammad Raisul Abedin Sutapa Barua Isolation and purification of glycoglycerolipids to induce apoptosis in breast cancer cells
description	Abstract Monogalactosyldiacylglycerol (MGDG) is the most abundant type of glycoglycerolipid found in the plant cell membrane and mostly in the chloroplast thylakoid membrane. The amphiphilic nature of MGDG is attractive in pharmaceutical fields for interaction with other biological molecules and hence exerting therapeutic anti-cancer, anti-viral, and anti-inflammatory activities. In this study, we investigated the therapeutic efficacy of cyanobacteria derived MGDG to inhibit breast cancer cell growth. MGDG was extracted from a cyanobacteria Synechocystis sp. PCC 6803 followed by a subsequent fractionation by column chromatographic technique. The purity and molecular structure of MGDG were analyzed by nuclear magnetic resonance (NMR) spectroscopy analysis. The presence of MGDG in the extracted fraction was further confirmed and quantified by high-performance liquid chromatography (HPLC). The anti-proliferation activity of the extracted MGDG molecule was tested against BT-474 and MDA-MB-231 breast cancer cell lines. The in vitro study showed that MGDG extracted from Synechocystis sp. PCC 6803 induced apoptosis in (70 ± 8) % of BT-474 (p < 0.001) and (58 ± 5) % of MDA-MB-231 cells (p < 0.001) using ~ 60 and 200 ng/ml of concentrations, respectively. The half-maximal inhibitory concentration, IC50 of MGDG extracted from Synechocystis sp. PCC 6803 were (27.2 ± 7.6) and (150 ± 70) ng/ml in BT-474 and MDA-MB-231 cell lines, respectively. Quantification of caspase-3/7 activity using flow cytometry showed (3.0 ± 0.4) and (2.1 ± 0.04)-fold (p < 0.001) higher protein expressions in the MGDG treated BT-474 and MDA-MB-231 cells, respectively than untreated controls conferring to the caspase-dependent apoptosis. The MGDG did not show any significant cytotoxic side effects in human dermal fibroblasts cells. A commercially available MGDG control did not induce any apoptotic cell death in cancer cells substantiating the potential of the MGDG extracted from Synechocystis sp. PCC 6803 for the treatment of breast cancer cells through the apoptosis-mediated pathway.
format	article
author	Muhammad Raisul Abedin Sutapa Barua
author_facet	Muhammad Raisul Abedin Sutapa Barua
author_sort	Muhammad Raisul Abedin
title	Isolation and purification of glycoglycerolipids to induce apoptosis in breast cancer cells
title_short	Isolation and purification of glycoglycerolipids to induce apoptosis in breast cancer cells
title_full	Isolation and purification of glycoglycerolipids to induce apoptosis in breast cancer cells
title_fullStr	Isolation and purification of glycoglycerolipids to induce apoptosis in breast cancer cells
title_full_unstemmed	Isolation and purification of glycoglycerolipids to induce apoptosis in breast cancer cells
title_sort	isolation and purification of glycoglycerolipids to induce apoptosis in breast cancer cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/77ed7f9e0d134a6facd16479c6af8752
work_keys_str_mv	AT muhammadraisulabedin isolationandpurificationofglycoglycerolipidstoinduceapoptosisinbreastcancercells AT sutapabarua isolationandpurificationofglycoglycerolipidstoinduceapoptosisinbreastcancercells
_version_	1718392149238611968

Isolation and purification of glycoglycerolipids to induce apoptosis in breast cancer cells

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