A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen

Tan Tai Nguyen,1 Sun Oh Bea,1 Dong Min Kim,2 Won Jung Yoon,3 Jin-Won Park,4 Seong Soo A An,1 Heongkyu Ju1,5,6 1Department of Bionano Technology, College of Bionano Technology, Gachon University, Seongnam, 2Department of Materials Science and Engineering, Hongik University, Sejong City, 3Department...

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Autores principales:	Nguyen TT, Bea SO, Kim DM, Yoon WJ, Park J-W, An SSA, Ju H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/77ee7d2e46934c38a84abbd8ef9cf6fa
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spelling	oai:doaj.org-article:77ee7d2e46934c38a84abbd8ef9cf6fa2021-12-02T03:11:32ZA regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen1178-2013https://doaj.org/article/77ee7d2e46934c38a84abbd8ef9cf6fa2015-08-01T00:00:00Zhttp://www.dovepress.com/a-regenerative-label-free-fiber-optic-sensor-using-surface-plasmon-res-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Tan Tai Nguyen,1 Sun Oh Bea,1 Dong Min Kim,2 Won Jung Yoon,3 Jin-Won Park,4 Seong Soo A An,1 Heongkyu Ju1,5,6 1Department of Bionano Technology, College of Bionano Technology, Gachon University, Seongnam, 2Department of Materials Science and Engineering, Hongik University, Sejong City, 3Department of Chemical and Bio Engineering, Gachon University, Seongnam, 4Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology, Seoul National University of Science and Technology, Seoul, 5Department of Nanophysics, College of Bionano Technology, Gachon University, Seongnam, 6Neuroscience Institute, Gil Hospital, Incheon, South Korea Purpose: We present the regenerative label-free fiber optical biosensor that exploits surface plasmon resonance for quantitative detection of fibrinogen (Fbg) extracted from human blood plasma.Materials and methods: The sensor head was made up of a multimode optical fiber with its polymer cladding replaced by metal composite of nanometer thickness made of silver, aluminum, and nickel. The Ni layer coated allowed a direct immobilization of histidine-tagged peptide (HP) on its metal surface without an additional cross-linker in between. On the coated HP layer, immunoglobulin G was then immobilized for specific capturing of Fbg.Results: We demonstrated a real-time quantitative detection of Fbg concentrations with limit of detection of ~10 ng/mL. The fact that the HP layer could be removed by imidazole with acid also permitted us to demonstrate the regeneration of the outermost metal surface of the sensor head for the sensor reusability.Conclusion: The sensor detection limit was estimated to be ~10 pM, which was believed to be sensitive enough for detecting Fbg during the clinical diagnosis of cardiovascular diseases, myocardial infarction, strokes, and Alzheimer’s diseases. Keywords: SPR, real-time assay, histidine-tagged peptide, protein sensingNguyen TTBea SOKim DMYoon WJPark J-WAn SSAJu HDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Special Issue on diverse applications in Nano-Theranostics, Pp 155-163 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Nguyen TT Bea SO Kim DM Yoon WJ Park J-W An SSA Ju H A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
description	Tan Tai Nguyen,1 Sun Oh Bea,1 Dong Min Kim,2 Won Jung Yoon,3 Jin-Won Park,4 Seong Soo A An,1 Heongkyu Ju1,5,6 1Department of Bionano Technology, College of Bionano Technology, Gachon University, Seongnam, 2Department of Materials Science and Engineering, Hongik University, Sejong City, 3Department of Chemical and Bio Engineering, Gachon University, Seongnam, 4Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology, Seoul National University of Science and Technology, Seoul, 5Department of Nanophysics, College of Bionano Technology, Gachon University, Seongnam, 6Neuroscience Institute, Gil Hospital, Incheon, South Korea Purpose: We present the regenerative label-free fiber optical biosensor that exploits surface plasmon resonance for quantitative detection of fibrinogen (Fbg) extracted from human blood plasma.Materials and methods: The sensor head was made up of a multimode optical fiber with its polymer cladding replaced by metal composite of nanometer thickness made of silver, aluminum, and nickel. The Ni layer coated allowed a direct immobilization of histidine-tagged peptide (HP) on its metal surface without an additional cross-linker in between. On the coated HP layer, immunoglobulin G was then immobilized for specific capturing of Fbg.Results: We demonstrated a real-time quantitative detection of Fbg concentrations with limit of detection of ~10 ng/mL. The fact that the HP layer could be removed by imidazole with acid also permitted us to demonstrate the regeneration of the outermost metal surface of the sensor head for the sensor reusability.Conclusion: The sensor detection limit was estimated to be ~10 pM, which was believed to be sensitive enough for detecting Fbg during the clinical diagnosis of cardiovascular diseases, myocardial infarction, strokes, and Alzheimer’s diseases. Keywords: SPR, real-time assay, histidine-tagged peptide, protein sensing
format	article
author	Nguyen TT Bea SO Kim DM Yoon WJ Park J-W An SSA Ju H
author_facet	Nguyen TT Bea SO Kim DM Yoon WJ Park J-W An SSA Ju H
author_sort	Nguyen TT
title	A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
title_short	A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
title_full	A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
title_fullStr	A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
title_full_unstemmed	A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
title_sort	regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/77ee7d2e46934c38a84abbd8ef9cf6fa
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A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen

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