Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand

Partitiviruses are one of the most prevalent double-stranded RNA viruses that have been identified mostly in filamentous fungi and plants. Partitiviruses generally infect host fungi asymptomatically but infrequently exert significant effect(s) on morphology and virulence, thus being considered a pot...

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Autores principales:	Sokty Neang, Santiti Bincader, Sansern Rangsuwan, Pisut Keawmanee, Soriya Rin, Lakha Salaipeth, Subha Das, Hideki Kondo, Nobuhiro Suzuki, Ikuo Sato, Daigo Takemoto, Chainarong Rattanakreetakul, Ratiya Pongpisutta, Masao Arakawa, Sotaro Chiba
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	rice aggregate sheath spot mycovirus Partitiviridae Rhizoctonia oryzae-sativae Rhizoctonia oryzae protoplasting Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/77f1487c45644122bb75393746302bbb
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spelling	oai:doaj.org-article:77f1487c45644122bb75393746302bbb2021-11-25T19:13:57ZOmnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand10.3390/v131122691999-4915https://doaj.org/article/77f1487c45644122bb75393746302bbb2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2269https://doaj.org/toc/1999-4915Partitiviruses are one of the most prevalent double-stranded RNA viruses that have been identified mostly in filamentous fungi and plants. Partitiviruses generally infect host fungi asymptomatically but infrequently exert significant effect(s) on morphology and virulence, thus being considered a potential source of biological control agents against pathogenic fungi. In this study, we performed a screening for mycoviruses of a collection of Thai isolates of rice fungal pathogen <i>Rhizoctonia oryzae-sativae</i>, a causal agent of rice aggregated sheath spot disease. As a result, 36% of tested isolates carried potentially viral double-stranded RNAs with sizes ranging from 2 to 3 kbp. By conventional cDNA library construction and RNA-seq, we determined six new alphapartitiviruses that infected three isolates: tentatively named Rhizoctonia oryzae-sativae partitivirus 1 to 6 (RosPV1-6). Furthermore, RT-PCR detection of each virus revealed their omnipresent nature in different <i>R. oryzae-sativae</i> isolates. Although virus-curing of basidiomycetous fungi is generally difficult, our repeated attempts successfully obtained virus-free (for RosPV1, RosPV2, and uncharacterized partitiviruses), isogenic strain of <i>R. oryzae-sativae</i> TSS190442. The virus-cured strain showed slightly faster colony growth on the synthetic media and severe symptom development on the rice sheath compared to its virus-infected counterpart. Overall, this study shed light on the distribution of partitiviruses in <i>R. oryzae-sativae</i> in a paddy environment and exemplified a virus-curing protocol that may be applicable for other basidiomycetous fungi.Sokty NeangSantiti BincaderSansern RangsuwanPisut KeawmaneeSoriya RinLakha SalaipethSubha DasHideki KondoNobuhiro SuzukiIkuo SatoDaigo TakemotoChainarong RattanakreetakulRatiya PongpisuttaMasao ArakawaSotaro ChibaMDPI AGarticlerice aggregate sheath spotmycovirusPartitiviridaeRhizoctonia oryzae-sativaeRhizoctonia oryzaeprotoplastingMicrobiologyQR1-502ENViruses, Vol 13, Iss 2269, p 2269 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rice aggregate sheath spot mycovirus Partitiviridae Rhizoctonia oryzae-sativae Rhizoctonia oryzae protoplasting Microbiology QR1-502
spellingShingle	rice aggregate sheath spot mycovirus Partitiviridae Rhizoctonia oryzae-sativae Rhizoctonia oryzae protoplasting Microbiology QR1-502 Sokty Neang Santiti Bincader Sansern Rangsuwan Pisut Keawmanee Soriya Rin Lakha Salaipeth Subha Das Hideki Kondo Nobuhiro Suzuki Ikuo Sato Daigo Takemoto Chainarong Rattanakreetakul Ratiya Pongpisutta Masao Arakawa Sotaro Chiba Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand
description	Partitiviruses are one of the most prevalent double-stranded RNA viruses that have been identified mostly in filamentous fungi and plants. Partitiviruses generally infect host fungi asymptomatically but infrequently exert significant effect(s) on morphology and virulence, thus being considered a potential source of biological control agents against pathogenic fungi. In this study, we performed a screening for mycoviruses of a collection of Thai isolates of rice fungal pathogen <i>Rhizoctonia oryzae-sativae</i>, a causal agent of rice aggregated sheath spot disease. As a result, 36% of tested isolates carried potentially viral double-stranded RNAs with sizes ranging from 2 to 3 kbp. By conventional cDNA library construction and RNA-seq, we determined six new alphapartitiviruses that infected three isolates: tentatively named Rhizoctonia oryzae-sativae partitivirus 1 to 6 (RosPV1-6). Furthermore, RT-PCR detection of each virus revealed their omnipresent nature in different <i>R. oryzae-sativae</i> isolates. Although virus-curing of basidiomycetous fungi is generally difficult, our repeated attempts successfully obtained virus-free (for RosPV1, RosPV2, and uncharacterized partitiviruses), isogenic strain of <i>R. oryzae-sativae</i> TSS190442. The virus-cured strain showed slightly faster colony growth on the synthetic media and severe symptom development on the rice sheath compared to its virus-infected counterpart. Overall, this study shed light on the distribution of partitiviruses in <i>R. oryzae-sativae</i> in a paddy environment and exemplified a virus-curing protocol that may be applicable for other basidiomycetous fungi.
format	article
author	Sokty Neang Santiti Bincader Sansern Rangsuwan Pisut Keawmanee Soriya Rin Lakha Salaipeth Subha Das Hideki Kondo Nobuhiro Suzuki Ikuo Sato Daigo Takemoto Chainarong Rattanakreetakul Ratiya Pongpisutta Masao Arakawa Sotaro Chiba
author_facet	Sokty Neang Santiti Bincader Sansern Rangsuwan Pisut Keawmanee Soriya Rin Lakha Salaipeth Subha Das Hideki Kondo Nobuhiro Suzuki Ikuo Sato Daigo Takemoto Chainarong Rattanakreetakul Ratiya Pongpisutta Masao Arakawa Sotaro Chiba
author_sort	Sokty Neang
title	Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand
title_short	Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand
title_full	Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand
title_fullStr	Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand
title_full_unstemmed	Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand
title_sort	omnipresence of partitiviruses in rice aggregate sheath spot symptom-associated fungal isolates from paddies in thailand
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/77f1487c45644122bb75393746302bbb
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Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand

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