Putative plasmid prophages of Bacillus cereus sensu lato may hold the key to undiscovered phage diversity

Abstract Bacteriophages are bacterial viruses and the most abundant biological entities on Earth. Temperate bacteriophages can form prophages stably maintained in the host population: they either integrate into the host genome or replicate as plasmids in the host cytoplasm. As shown, tailed temperat...

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Autores principales:	Emma G. Piligrimova, Olesya A. Kazantseva, Andrey N. Kazantsev, Nikita A. Nikulin, Anna V. Skorynina, Olga N. Koposova, Andrey M. Shadrin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/77f32a3d1f514f72b4ef7a30efe4f907
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spelling	oai:doaj.org-article:77f32a3d1f514f72b4ef7a30efe4f9072021-12-02T18:15:24ZPutative plasmid prophages of Bacillus cereus sensu lato may hold the key to undiscovered phage diversity10.1038/s41598-021-87111-32045-2322https://doaj.org/article/77f32a3d1f514f72b4ef7a30efe4f9072021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87111-3https://doaj.org/toc/2045-2322Abstract Bacteriophages are bacterial viruses and the most abundant biological entities on Earth. Temperate bacteriophages can form prophages stably maintained in the host population: they either integrate into the host genome or replicate as plasmids in the host cytoplasm. As shown, tailed temperate bacteriophages may form circular plasmid prophages in many bacterial species of the taxa Firmicutes, Gammaproteobacteria and Spirochaetes. The actual number of such prophages is thought to be underestimated for two main reasons: first, in bacterial whole genome-sequencing assemblies, they are difficult to distinguish from actual plasmids; second, there is an absence of experimental studies which are vital to confirm their existence. In Firmicutes, such prophages appear to be especially numerous. In the present study, we identified 23 genomes from species of the Bacillus cereus group that were deposited in GenBank as plasmids and may belong to plasmid prophages with little or no homology to known viruses. We consider these putative prophages worth experimental assays since it will broaden our knowledge of phage diversity and suggest that more attention be paid to such molecules in all bacterial sequencing projects as this will help in identifying previously unknown phages.Emma G. PiligrimovaOlesya A. KazantsevaAndrey N. KazantsevNikita A. NikulinAnna V. SkoryninaOlga N. KoposovaAndrey M. ShadrinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emma G. Piligrimova Olesya A. Kazantseva Andrey N. Kazantsev Nikita A. Nikulin Anna V. Skorynina Olga N. Koposova Andrey M. Shadrin Putative plasmid prophages of Bacillus cereus sensu lato may hold the key to undiscovered phage diversity
description	Abstract Bacteriophages are bacterial viruses and the most abundant biological entities on Earth. Temperate bacteriophages can form prophages stably maintained in the host population: they either integrate into the host genome or replicate as plasmids in the host cytoplasm. As shown, tailed temperate bacteriophages may form circular plasmid prophages in many bacterial species of the taxa Firmicutes, Gammaproteobacteria and Spirochaetes. The actual number of such prophages is thought to be underestimated for two main reasons: first, in bacterial whole genome-sequencing assemblies, they are difficult to distinguish from actual plasmids; second, there is an absence of experimental studies which are vital to confirm their existence. In Firmicutes, such prophages appear to be especially numerous. In the present study, we identified 23 genomes from species of the Bacillus cereus group that were deposited in GenBank as plasmids and may belong to plasmid prophages with little or no homology to known viruses. We consider these putative prophages worth experimental assays since it will broaden our knowledge of phage diversity and suggest that more attention be paid to such molecules in all bacterial sequencing projects as this will help in identifying previously unknown phages.
format	article
author	Emma G. Piligrimova Olesya A. Kazantseva Andrey N. Kazantsev Nikita A. Nikulin Anna V. Skorynina Olga N. Koposova Andrey M. Shadrin
author_facet	Emma G. Piligrimova Olesya A. Kazantseva Andrey N. Kazantsev Nikita A. Nikulin Anna V. Skorynina Olga N. Koposova Andrey M. Shadrin
author_sort	Emma G. Piligrimova
title	Putative plasmid prophages of Bacillus cereus sensu lato may hold the key to undiscovered phage diversity
title_short	Putative plasmid prophages of Bacillus cereus sensu lato may hold the key to undiscovered phage diversity
title_full	Putative plasmid prophages of Bacillus cereus sensu lato may hold the key to undiscovered phage diversity
title_fullStr	Putative plasmid prophages of Bacillus cereus sensu lato may hold the key to undiscovered phage diversity
title_full_unstemmed	Putative plasmid prophages of Bacillus cereus sensu lato may hold the key to undiscovered phage diversity
title_sort	putative plasmid prophages of bacillus cereus sensu lato may hold the key to undiscovered phage diversity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/77f32a3d1f514f72b4ef7a30efe4f907
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Putative plasmid prophages of Bacillus cereus sensu lato may hold the key to undiscovered phage diversity

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