Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis

Abstract Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme environmental conditions include hypersalinity, high UV incidence, a...

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Autores principales:	Daniel Kurth, Ariel Amadio, Omar F. Ordoñez, Virginia H. Albarracín, Wolfgang Gärtner, María E. Farías
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/77f651e76b8941f6b72c2396f6ac571f
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spelling	oai:doaj.org-article:77f651e76b8941f6b72c2396f6ac571f2021-12-02T15:06:07ZArsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis10.1038/s41598-017-00896-02045-2322https://doaj.org/article/77f651e76b8941f6b72c2396f6ac571f2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00896-0https://doaj.org/toc/2045-2322Abstract Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme environmental conditions include hypersalinity, high UV incidence, and high arsenic content, among others. After Socompa’s stromatolite microbial communities were analysed by metagenomic DNA sequencing, taxonomic classification showed dominance of Proteobacteria, Bacteroidetes and Firmicutes, and a remarkably high number of unclassified sequences. A functional analysis indicated that carbon fixation might occur not only by the Calvin-Benson cycle, but also through alternative pathways such as the reverse TCA cycle, and the reductive acetyl-CoA pathway. Deltaproteobacteria were involved both in sulfate reduction and nitrogen fixation. Significant differences were found when comparing the Socompa stromatolite metagenome to the Shark Bay (Australia) smooth mat metagenome: namely, those involving stress related processes, particularly, arsenic resistance. An in-depth analysis revealed a surprisingly diverse metabolism comprising all known types of As resistance and energy generating pathways. While the ars operon was the main mechanism, an important abundance of arsM genes was observed in selected phyla. The data resulting from this work will prove a cornerstone for further studies on this rare microbial community.Daniel KurthAriel AmadioOmar F. OrdoñezVirginia H. AlbarracínWolfgang GärtnerMaría E. FaríasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Kurth Ariel Amadio Omar F. Ordoñez Virginia H. Albarracín Wolfgang Gärtner María E. Farías Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
description	Abstract Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme environmental conditions include hypersalinity, high UV incidence, and high arsenic content, among others. After Socompa’s stromatolite microbial communities were analysed by metagenomic DNA sequencing, taxonomic classification showed dominance of Proteobacteria, Bacteroidetes and Firmicutes, and a remarkably high number of unclassified sequences. A functional analysis indicated that carbon fixation might occur not only by the Calvin-Benson cycle, but also through alternative pathways such as the reverse TCA cycle, and the reductive acetyl-CoA pathway. Deltaproteobacteria were involved both in sulfate reduction and nitrogen fixation. Significant differences were found when comparing the Socompa stromatolite metagenome to the Shark Bay (Australia) smooth mat metagenome: namely, those involving stress related processes, particularly, arsenic resistance. An in-depth analysis revealed a surprisingly diverse metabolism comprising all known types of As resistance and energy generating pathways. While the ars operon was the main mechanism, an important abundance of arsM genes was observed in selected phyla. The data resulting from this work will prove a cornerstone for further studies on this rare microbial community.
format	article
author	Daniel Kurth Ariel Amadio Omar F. Ordoñez Virginia H. Albarracín Wolfgang Gärtner María E. Farías
author_facet	Daniel Kurth Ariel Amadio Omar F. Ordoñez Virginia H. Albarracín Wolfgang Gärtner María E. Farías
author_sort	Daniel Kurth
title	Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title_short	Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title_full	Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title_fullStr	Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title_full_unstemmed	Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title_sort	arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/77f651e76b8941f6b72c2396f6ac571f
work_keys_str_mv	AT danielkurth arsenicmetabolisminhighaltitudemodernstromatolitesrevealedbymetagenomicanalysis AT arielamadio arsenicmetabolisminhighaltitudemodernstromatolitesrevealedbymetagenomicanalysis AT omarfordonez arsenicmetabolisminhighaltitudemodernstromatolitesrevealedbymetagenomicanalysis AT virginiahalbarracin arsenicmetabolisminhighaltitudemodernstromatolitesrevealedbymetagenomicanalysis AT wolfganggartner arsenicmetabolisminhighaltitudemodernstromatolitesrevealedbymetagenomicanalysis AT mariaefarias arsenicmetabolisminhighaltitudemodernstromatolitesrevealedbymetagenomicanalysis
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Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis

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