<i>S. pyogenes</i> M49-16 arginine deiminase inhibits proliferative activity of human peripheral blood lymphocytes

Arginine deiminase is one of three enzymes constituting the arginine deiminase system in bacteria. It was demonstrated that arginine deiminase exerts anti-proliferative effects on some primary and immortalized mouse and human cells. It is assumed that the inhibitory effect of arginine deiminase on c...

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Autores principales:	E. A. Starickova, T. A. Leveshko, D. V. Churakina, I. V. Kudryavtsev, L. A. Burova, I. S. Freidlin
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2021
Materias:	arginine deiminase streptococcus pyogenes lymphocytes cfse proliferation cd45 Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/77fc943642c7427889db8aa2dab327ae
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spelling	oai:doaj.org-article:77fc943642c7427889db8aa2dab327ae2021-11-22T07:09:54Z<i>S. pyogenes</i> M49-16 arginine deiminase inhibits proliferative activity of human peripheral blood lymphocytes2220-76192313-739810.15789/2220-7619-ADF-1363https://doaj.org/article/77fc943642c7427889db8aa2dab327ae2021-03-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1363https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Arginine deiminase is one of three enzymes constituting the arginine deiminase system in bacteria. It was demonstrated that arginine deiminase exerts anti-proliferative effects on some primary and immortalized mouse and human cells. It is assumed that the inhibitory effect of arginine deiminase on cell proliferation might be related to its ability to result in the arginine exhaustion. T-lymphocytes depend on arginine for proliferation, T-cell receptor complex expression, and the differentiation of memory cells. The aim of the current study was to investigate an impact streptococcal arginine deiminase on functions of human peripheral blood lymphocytes. For this, we comparatively analyzed effects of Supernatant of Destroyed Streptococcal Cells (SDSCs) derived from parental strain S. pyogenes M49-16 and its isogenic mutant S. pyogenes M49-16delArcA bearing inactivated arginine deiminase gene (ArcA) on immune cell functions. An impact of supernatants on cell viability was estimated by staining with DAPI dye. Cell proliferation was assessed by MTT-test and flow cytometry by using the method based on intracellular protein staining with vital fluorescent CFSE (carboxyfluorescein succinimidyl ester) dye. In addition, the level of lymphocyte tyrosine phosphatase CD45 expression in various culturing conditions was evaluated. It was demonstrated that S. pyogenes M49-16 SDSCs had no impact on cells viability. Parental strain-derived SDSC exerted virtually no effect on intact cells proliferation, but considerably suppressed ConA-induced cell proliferation. At the same time, mutant strain-derived SDSC significantly stimulated spontaneous cell proliferation, but not that one after mitogen exposure. It was observed that increased proliferation was accompanied by upregulated CD45 expression, although it was not significant in all cases. These data allow to conclude that bacterial arginine deiminase could be one of pathogenicity factors able to limit lymphocyte proliferation and immune response and could be a part of pathogen strategy to suppress immune response in order to improve bacterial growth and dissemination.E. A. StarickovaT. A. LeveshkoD. V. ChurakinaI. V. KudryavtsevL. A. BurovaI. S. FreidlinSankt-Peterburg : NIIÈM imeni Pasteraarticlearginine deiminasestreptococcus pyogeneslymphocytescfseproliferationcd45Infectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 11, Iss 2, Pp 349-356 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	arginine deiminase streptococcus pyogenes lymphocytes cfse proliferation cd45 Infectious and parasitic diseases RC109-216
spellingShingle	arginine deiminase streptococcus pyogenes lymphocytes cfse proliferation cd45 Infectious and parasitic diseases RC109-216 E. A. Starickova T. A. Leveshko D. V. Churakina I. V. Kudryavtsev L. A. Burova I. S. Freidlin <i>S. pyogenes</i> M49-16 arginine deiminase inhibits proliferative activity of human peripheral blood lymphocytes
description	Arginine deiminase is one of three enzymes constituting the arginine deiminase system in bacteria. It was demonstrated that arginine deiminase exerts anti-proliferative effects on some primary and immortalized mouse and human cells. It is assumed that the inhibitory effect of arginine deiminase on cell proliferation might be related to its ability to result in the arginine exhaustion. T-lymphocytes depend on arginine for proliferation, T-cell receptor complex expression, and the differentiation of memory cells. The aim of the current study was to investigate an impact streptococcal arginine deiminase on functions of human peripheral blood lymphocytes. For this, we comparatively analyzed effects of Supernatant of Destroyed Streptococcal Cells (SDSCs) derived from parental strain S. pyogenes M49-16 and its isogenic mutant S. pyogenes M49-16delArcA bearing inactivated arginine deiminase gene (ArcA) on immune cell functions. An impact of supernatants on cell viability was estimated by staining with DAPI dye. Cell proliferation was assessed by MTT-test and flow cytometry by using the method based on intracellular protein staining with vital fluorescent CFSE (carboxyfluorescein succinimidyl ester) dye. In addition, the level of lymphocyte tyrosine phosphatase CD45 expression in various culturing conditions was evaluated. It was demonstrated that S. pyogenes M49-16 SDSCs had no impact on cells viability. Parental strain-derived SDSC exerted virtually no effect on intact cells proliferation, but considerably suppressed ConA-induced cell proliferation. At the same time, mutant strain-derived SDSC significantly stimulated spontaneous cell proliferation, but not that one after mitogen exposure. It was observed that increased proliferation was accompanied by upregulated CD45 expression, although it was not significant in all cases. These data allow to conclude that bacterial arginine deiminase could be one of pathogenicity factors able to limit lymphocyte proliferation and immune response and could be a part of pathogen strategy to suppress immune response in order to improve bacterial growth and dissemination.
format	article
author	E. A. Starickova T. A. Leveshko D. V. Churakina I. V. Kudryavtsev L. A. Burova I. S. Freidlin
author_facet	E. A. Starickova T. A. Leveshko D. V. Churakina I. V. Kudryavtsev L. A. Burova I. S. Freidlin
author_sort	E. A. Starickova
title	<i>S. pyogenes</i> M49-16 arginine deiminase inhibits proliferative activity of human peripheral blood lymphocytes
title_short	<i>S. pyogenes</i> M49-16 arginine deiminase inhibits proliferative activity of human peripheral blood lymphocytes
title_full	<i>S. pyogenes</i> M49-16 arginine deiminase inhibits proliferative activity of human peripheral blood lymphocytes
title_fullStr	<i>S. pyogenes</i> M49-16 arginine deiminase inhibits proliferative activity of human peripheral blood lymphocytes
title_full_unstemmed	<i>S. pyogenes</i> M49-16 arginine deiminase inhibits proliferative activity of human peripheral blood lymphocytes
title_sort	<i>s. pyogenes</i> m49-16 arginine deiminase inhibits proliferative activity of human peripheral blood lymphocytes
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2021
url	https://doaj.org/article/77fc943642c7427889db8aa2dab327ae
work_keys_str_mv	AT eastarickova ispyogenesim4916argininedeiminaseinhibitsproliferativeactivityofhumanperipheralbloodlymphocytes AT taleveshko ispyogenesim4916argininedeiminaseinhibitsproliferativeactivityofhumanperipheralbloodlymphocytes AT dvchurakina ispyogenesim4916argininedeiminaseinhibitsproliferativeactivityofhumanperipheralbloodlymphocytes AT ivkudryavtsev ispyogenesim4916argininedeiminaseinhibitsproliferativeactivityofhumanperipheralbloodlymphocytes AT laburova ispyogenesim4916argininedeiminaseinhibitsproliferativeactivityofhumanperipheralbloodlymphocytes AT isfreidlin ispyogenesim4916argininedeiminaseinhibitsproliferativeactivityofhumanperipheralbloodlymphocytes
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<i>S. pyogenes</i> M49-16 arginine deiminase inhibits proliferative activity of human peripheral blood lymphocytes

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