Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions

Proteins associate via weak and transient interactions that are challenging to identify in vivo. Here, the authors use a genetically encoded chemical cross-linker to covalently lock interacting proteins in live cells, allowing them to identify the captured proteins by mass spectrometry.

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Autores principales:	Bing Yang, Shibing Tang, Cheng Ma, Shang-Tong Li, Guang-Can Shao, Bobo Dang, William F. DeGrado, Meng-Qiu Dong, Peng George Wang, Sheng Ding, Lei Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/780166a230784b39849b98e73164024f
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spelling	oai:doaj.org-article:780166a230784b39849b98e73164024f2021-12-02T14:40:36ZSpontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions10.1038/s41467-017-02409-z2041-1723https://doaj.org/article/780166a230784b39849b98e73164024f2017-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-02409-zhttps://doaj.org/toc/2041-1723Proteins associate via weak and transient interactions that are challenging to identify in vivo. Here, the authors use a genetically encoded chemical cross-linker to covalently lock interacting proteins in live cells, allowing them to identify the captured proteins by mass spectrometry.Bing YangShibing TangCheng MaShang-Tong LiGuang-Can ShaoBobo DangWilliam F. DeGradoMeng-Qiu DongPeng George WangSheng DingLei WangNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Bing Yang Shibing Tang Cheng Ma Shang-Tong Li Guang-Can Shao Bobo Dang William F. DeGrado Meng-Qiu Dong Peng George Wang Sheng Ding Lei Wang Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions
description	Proteins associate via weak and transient interactions that are challenging to identify in vivo. Here, the authors use a genetically encoded chemical cross-linker to covalently lock interacting proteins in live cells, allowing them to identify the captured proteins by mass spectrometry.
format	article
author	Bing Yang Shibing Tang Cheng Ma Shang-Tong Li Guang-Can Shao Bobo Dang William F. DeGrado Meng-Qiu Dong Peng George Wang Sheng Ding Lei Wang
author_facet	Bing Yang Shibing Tang Cheng Ma Shang-Tong Li Guang-Can Shao Bobo Dang William F. DeGrado Meng-Qiu Dong Peng George Wang Sheng Ding Lei Wang
author_sort	Bing Yang
title	Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions
title_short	Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions
title_full	Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions
title_fullStr	Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions
title_full_unstemmed	Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions
title_sort	spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/780166a230784b39849b98e73164024f
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Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions

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