CNOT6/6L-mediated mRNA degradation in ovarian granulosa cells is a key mechanism of gonadotropin-triggered follicle development

Summary: CCR4-NOT deadenylase is a major regulator of mRNA turnover. It contains two heterogeneous catalytic subunits CNOT7/8 and CNOT6/6L in vertebrates. The physiological function of each catalytic subunit is unclear due to the gene redundancy. In this study, Cnot6/6l double knockout mice are gene...

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Autores principales:	Xing-Xing Dai, Zhi-Yan Jiang, Yun-Wen Wu, Qian-Qian Sha, Yang Liu, Jia-Yi Ding, Wen-Dong Xi, Jing Li, Heng-Yu Fan
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	follicle-stimulating hormone mRNA stability mRNA translation PI3K signaling pathway female reproduction ovulation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7804dcdc6b2041b8839bd5309aa88b29
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spelling	oai:doaj.org-article:7804dcdc6b2041b8839bd5309aa88b292021-11-18T04:47:54ZCNOT6/6L-mediated mRNA degradation in ovarian granulosa cells is a key mechanism of gonadotropin-triggered follicle development2211-124710.1016/j.celrep.2021.110007https://doaj.org/article/7804dcdc6b2041b8839bd5309aa88b292021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2211124721014856https://doaj.org/toc/2211-1247Summary: CCR4-NOT deadenylase is a major regulator of mRNA turnover. It contains two heterogeneous catalytic subunits CNOT7/8 and CNOT6/6L in vertebrates. The physiological function of each catalytic subunit is unclear due to the gene redundancy. In this study, Cnot6/6l double knockout mice are generated. Cnot6l−/− female mice are infertile, with poor ovarian responses to gonadotropins. Follicle-stimulating hormone (FSH) stimulates the transcription and translation of Cnot6 and Cnot6l in ovarian granulosa cells. CNOT6/6L function as key effectors of FSH in granulosa cells and trigger the clearance of specific transcripts in granulosa cells during preantral to antral follicle transition. These results demonstrate that FSH modulates granulosa cell function by stimulating selective translational activation and degradation of existing mRNAs, in addition to inducing de novo gene transcription. Meanwhile, this study provides in vivo evidence that CNOT6/6L-mediated mRNA deadenylation is dispensable in most somatic cell types, but is essential for female reproductive endocrine regulation.Xing-Xing DaiZhi-Yan JiangYun-Wen WuQian-Qian ShaYang LiuJia-Yi DingWen-Dong XiJing LiHeng-Yu FanElsevierarticlefollicle-stimulating hormonemRNA stabilitymRNA translationPI3K signaling pathwayfemale reproductionovulationBiology (General)QH301-705.5ENCell Reports, Vol 37, Iss 7, Pp 110007- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	follicle-stimulating hormone mRNA stability mRNA translation PI3K signaling pathway female reproduction ovulation Biology (General) QH301-705.5
spellingShingle	follicle-stimulating hormone mRNA stability mRNA translation PI3K signaling pathway female reproduction ovulation Biology (General) QH301-705.5 Xing-Xing Dai Zhi-Yan Jiang Yun-Wen Wu Qian-Qian Sha Yang Liu Jia-Yi Ding Wen-Dong Xi Jing Li Heng-Yu Fan CNOT6/6L-mediated mRNA degradation in ovarian granulosa cells is a key mechanism of gonadotropin-triggered follicle development
description	Summary: CCR4-NOT deadenylase is a major regulator of mRNA turnover. It contains two heterogeneous catalytic subunits CNOT7/8 and CNOT6/6L in vertebrates. The physiological function of each catalytic subunit is unclear due to the gene redundancy. In this study, Cnot6/6l double knockout mice are generated. Cnot6l−/− female mice are infertile, with poor ovarian responses to gonadotropins. Follicle-stimulating hormone (FSH) stimulates the transcription and translation of Cnot6 and Cnot6l in ovarian granulosa cells. CNOT6/6L function as key effectors of FSH in granulosa cells and trigger the clearance of specific transcripts in granulosa cells during preantral to antral follicle transition. These results demonstrate that FSH modulates granulosa cell function by stimulating selective translational activation and degradation of existing mRNAs, in addition to inducing de novo gene transcription. Meanwhile, this study provides in vivo evidence that CNOT6/6L-mediated mRNA deadenylation is dispensable in most somatic cell types, but is essential for female reproductive endocrine regulation.
format	article
author	Xing-Xing Dai Zhi-Yan Jiang Yun-Wen Wu Qian-Qian Sha Yang Liu Jia-Yi Ding Wen-Dong Xi Jing Li Heng-Yu Fan
author_facet	Xing-Xing Dai Zhi-Yan Jiang Yun-Wen Wu Qian-Qian Sha Yang Liu Jia-Yi Ding Wen-Dong Xi Jing Li Heng-Yu Fan
author_sort	Xing-Xing Dai
title	CNOT6/6L-mediated mRNA degradation in ovarian granulosa cells is a key mechanism of gonadotropin-triggered follicle development
title_short	CNOT6/6L-mediated mRNA degradation in ovarian granulosa cells is a key mechanism of gonadotropin-triggered follicle development
title_full	CNOT6/6L-mediated mRNA degradation in ovarian granulosa cells is a key mechanism of gonadotropin-triggered follicle development
title_fullStr	CNOT6/6L-mediated mRNA degradation in ovarian granulosa cells is a key mechanism of gonadotropin-triggered follicle development
title_full_unstemmed	CNOT6/6L-mediated mRNA degradation in ovarian granulosa cells is a key mechanism of gonadotropin-triggered follicle development
title_sort	cnot6/6l-mediated mrna degradation in ovarian granulosa cells is a key mechanism of gonadotropin-triggered follicle development
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/7804dcdc6b2041b8839bd5309aa88b29
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CNOT6/6L-mediated mRNA degradation in ovarian granulosa cells is a key mechanism of gonadotropin-triggered follicle development

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