Increased Lipid Peroxidation May Be Linked to Ferritin Levels Elevation in Adult-Onset Still’s Disease

Lipid peroxidation (LPO) and hyper-ferritinemia are involved in inflammatory responses. Although hyper-ferritinemia is a characteristic of AOSD, its link to LPO remains unclear. We investigated the association between LPO and ferritin expression, and evaluated the relationship between LPO-related me...

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Autores principales:	Po-Ku Chen, Kai-Jieh Yeo, Po-Hao Huang, Shih-Hsin Chang, Ching-Kun Chang, Joung-Liang Lan, Der-Yuan Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	lipid peroxidation (LPO) LPO inducer LPO-related metabolites ferritin adult-onset Still’s disease (AOSD) Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/781136455b3c4199b6b8c4ec904b7b33
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spelling	oai:doaj.org-article:781136455b3c4199b6b8c4ec904b7b332021-11-25T16:48:23ZIncreased Lipid Peroxidation May Be Linked to Ferritin Levels Elevation in Adult-Onset Still’s Disease10.3390/biomedicines91115082227-9059https://doaj.org/article/781136455b3c4199b6b8c4ec904b7b332021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1508https://doaj.org/toc/2227-9059Lipid peroxidation (LPO) and hyper-ferritinemia are involved in inflammatory responses. Although hyper-ferritinemia is a characteristic of AOSD, its link to LPO remains unclear. We investigated the association between LPO and ferritin expression, and evaluated the relationship between LPO-related metabolites and inflammatory parameters. Mean fluorescence intensity (MFI) of LPO (C11-Biodipy<sup>581/591</sup>)-expressing PBMCs/monocytes in AOSD patients and healthy control (HC) subjects was determined by flow-cytometry analysis. Expression of ferritin and cytokines on PBMCs/macrophages was examined by immunoblotting. Plasma levels of LPO-related metabolites and cytokines were determined by ELISA and the MULTIPLEX platform, respectively. LPO MFI on PBMCs/monocytes were significantly higher in patients (median 4456 and 9091, respectively) compared with HC (1900, <i>p</i> < 0.05, and 4551, <i>p</i> < 0.01, respectively). Patients had higher ferritin expression on PBMCs (mean fold, 1.02) than HC (0.55, <i>p</i> < 0.05). Their ferritin expression levels on PBMCs stimulated with LPO inducers erastin or RSL3 (2.47 or 1.61, respectively) were higher than HC (0.84, <i>p</i> < 0.05, or 0.74, <i>p</i> < 0.01). Ferritin expression on erastin-treated/IL-1β-treated macrophages from patients were higher than those from HC (<i>p</i> < 0.001). The elevated levels of LPO-related metabolites, including malondialdehyde and 4-hydroxyalkenals, were positively correlated with disease activity scores, suggesting LPO involvement in AOSD pathogenesis. Increased ferritin expression on PBMCs/macrophages stimulated with LPO inducers indicates a link between LPO and elevated ferritin.Po-Ku ChenKai-Jieh YeoPo-Hao HuangShih-Hsin ChangChing-Kun ChangJoung-Liang LanDer-Yuan ChenMDPI AGarticlelipid peroxidation (LPO)LPO inducerLPO-related metabolitesferritinadult-onset Still’s disease (AOSD)Biology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1508, p 1508 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lipid peroxidation (LPO) LPO inducer LPO-related metabolites ferritin adult-onset Still’s disease (AOSD) Biology (General) QH301-705.5
spellingShingle	lipid peroxidation (LPO) LPO inducer LPO-related metabolites ferritin adult-onset Still’s disease (AOSD) Biology (General) QH301-705.5 Po-Ku Chen Kai-Jieh Yeo Po-Hao Huang Shih-Hsin Chang Ching-Kun Chang Joung-Liang Lan Der-Yuan Chen Increased Lipid Peroxidation May Be Linked to Ferritin Levels Elevation in Adult-Onset Still’s Disease
description	Lipid peroxidation (LPO) and hyper-ferritinemia are involved in inflammatory responses. Although hyper-ferritinemia is a characteristic of AOSD, its link to LPO remains unclear. We investigated the association between LPO and ferritin expression, and evaluated the relationship between LPO-related metabolites and inflammatory parameters. Mean fluorescence intensity (MFI) of LPO (C11-Biodipy<sup>581/591</sup>)-expressing PBMCs/monocytes in AOSD patients and healthy control (HC) subjects was determined by flow-cytometry analysis. Expression of ferritin and cytokines on PBMCs/macrophages was examined by immunoblotting. Plasma levels of LPO-related metabolites and cytokines were determined by ELISA and the MULTIPLEX platform, respectively. LPO MFI on PBMCs/monocytes were significantly higher in patients (median 4456 and 9091, respectively) compared with HC (1900, <i>p</i> < 0.05, and 4551, <i>p</i> < 0.01, respectively). Patients had higher ferritin expression on PBMCs (mean fold, 1.02) than HC (0.55, <i>p</i> < 0.05). Their ferritin expression levels on PBMCs stimulated with LPO inducers erastin or RSL3 (2.47 or 1.61, respectively) were higher than HC (0.84, <i>p</i> < 0.05, or 0.74, <i>p</i> < 0.01). Ferritin expression on erastin-treated/IL-1β-treated macrophages from patients were higher than those from HC (<i>p</i> < 0.001). The elevated levels of LPO-related metabolites, including malondialdehyde and 4-hydroxyalkenals, were positively correlated with disease activity scores, suggesting LPO involvement in AOSD pathogenesis. Increased ferritin expression on PBMCs/macrophages stimulated with LPO inducers indicates a link between LPO and elevated ferritin.
format	article
author	Po-Ku Chen Kai-Jieh Yeo Po-Hao Huang Shih-Hsin Chang Ching-Kun Chang Joung-Liang Lan Der-Yuan Chen
author_facet	Po-Ku Chen Kai-Jieh Yeo Po-Hao Huang Shih-Hsin Chang Ching-Kun Chang Joung-Liang Lan Der-Yuan Chen
author_sort	Po-Ku Chen
title	Increased Lipid Peroxidation May Be Linked to Ferritin Levels Elevation in Adult-Onset Still’s Disease
title_short	Increased Lipid Peroxidation May Be Linked to Ferritin Levels Elevation in Adult-Onset Still’s Disease
title_full	Increased Lipid Peroxidation May Be Linked to Ferritin Levels Elevation in Adult-Onset Still’s Disease
title_fullStr	Increased Lipid Peroxidation May Be Linked to Ferritin Levels Elevation in Adult-Onset Still’s Disease
title_full_unstemmed	Increased Lipid Peroxidation May Be Linked to Ferritin Levels Elevation in Adult-Onset Still’s Disease
title_sort	increased lipid peroxidation may be linked to ferritin levels elevation in adult-onset still’s disease
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/781136455b3c4199b6b8c4ec904b7b33
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Increased Lipid Peroxidation May Be Linked to Ferritin Levels Elevation in Adult-Onset Still’s Disease

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