Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)

Abstract We conducted a study to identify the fecal metabolite profile and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fluid were collected before and at slaughter, r...

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Autores principales:	Jessica Moraes Malheiros, Banny Silva Barbosa Correia, Caroline Ceribeli, Daniel Rodrigues Cardoso, Luiz Alberto Colnago, Stanislau Bogusz Junior, James Mark Reecy, Gerson Barreto Mourão, Luiz Lehmann Coutinho, Julio Cesar Pascale Palhares, Alexandre Berndt, Luciana Correia de Almeida Regitano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7818606b0b7c4c0e99de79a46b91d62b
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spelling	oai:doaj.org-article:7818606b0b7c4c0e99de79a46b91d62b2021-12-02T17:13:27ZComparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)10.1038/s41598-021-92179-y2045-2322https://doaj.org/article/7818606b0b7c4c0e99de79a46b91d62b2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92179-yhttps://doaj.org/toc/2045-2322Abstract We conducted a study to identify the fecal metabolite profile and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fluid were collected before and at slaughter, respectively. The metabolomics analysis indicated 49 common polar metabolites in the rumen and feces. Acetate, propionate, and butyrate were the most abundant polar metabolites in both bio-samples. The rumen presented significantly higher concentrations of the polar compounds when compared to feces (P < 0.05); even though, fecal metabolites presented an accentuated representability of the ruminal fluid metabolites. All fatty acids present in the ruminal fluid were also observed in the feces, except for C20:2n6 and C20:4n6. The identified metabolites offer information on the main metabolic pathways (higher impact factor and P < 0.05), as synthesis and degradation of ketone bodies; the alanine, aspartate and glutamate metabolisms, the glycine, serine; and threonine metabolism and the pyruvate metabolism. The findings reported herein on the close relationship between the ruminal fluid and feces metabolic profiles may offer new metabolic information, in addition to facilitating the sampling for metabolism investigation in animal production and health routines.Jessica Moraes MalheirosBanny Silva Barbosa CorreiaCaroline CeribeliDaniel Rodrigues CardosoLuiz Alberto ColnagoStanislau Bogusz JuniorJames Mark ReecyGerson Barreto MourãoLuiz Lehmann CoutinhoJulio Cesar Pascale PalharesAlexandre BerndtLuciana Correia de Almeida RegitanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jessica Moraes Malheiros Banny Silva Barbosa Correia Caroline Ceribeli Daniel Rodrigues Cardoso Luiz Alberto Colnago Stanislau Bogusz Junior James Mark Reecy Gerson Barreto Mourão Luiz Lehmann Coutinho Julio Cesar Pascale Palhares Alexandre Berndt Luciana Correia de Almeida Regitano Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)
description	Abstract We conducted a study to identify the fecal metabolite profile and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fluid were collected before and at slaughter, respectively. The metabolomics analysis indicated 49 common polar metabolites in the rumen and feces. Acetate, propionate, and butyrate were the most abundant polar metabolites in both bio-samples. The rumen presented significantly higher concentrations of the polar compounds when compared to feces (P < 0.05); even though, fecal metabolites presented an accentuated representability of the ruminal fluid metabolites. All fatty acids present in the ruminal fluid were also observed in the feces, except for C20:2n6 and C20:4n6. The identified metabolites offer information on the main metabolic pathways (higher impact factor and P < 0.05), as synthesis and degradation of ketone bodies; the alanine, aspartate and glutamate metabolisms, the glycine, serine; and threonine metabolism and the pyruvate metabolism. The findings reported herein on the close relationship between the ruminal fluid and feces metabolic profiles may offer new metabolic information, in addition to facilitating the sampling for metabolism investigation in animal production and health routines.
format	article
author	Jessica Moraes Malheiros Banny Silva Barbosa Correia Caroline Ceribeli Daniel Rodrigues Cardoso Luiz Alberto Colnago Stanislau Bogusz Junior James Mark Reecy Gerson Barreto Mourão Luiz Lehmann Coutinho Julio Cesar Pascale Palhares Alexandre Berndt Luciana Correia de Almeida Regitano
author_facet	Jessica Moraes Malheiros Banny Silva Barbosa Correia Caroline Ceribeli Daniel Rodrigues Cardoso Luiz Alberto Colnago Stanislau Bogusz Junior James Mark Reecy Gerson Barreto Mourão Luiz Lehmann Coutinho Julio Cesar Pascale Palhares Alexandre Berndt Luciana Correia de Almeida Regitano
author_sort	Jessica Moraes Malheiros
title	Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)
title_short	Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)
title_full	Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)
title_fullStr	Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)
title_full_unstemmed	Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)
title_sort	comparative untargeted metabolome analysis of ruminal fluid and feces of nelore steers (bos indicus)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7818606b0b7c4c0e99de79a46b91d62b
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Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)

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